A simple micro-assay for inorganic phosphate
Modification of two assay procedures (Van Belle, H. (1970) Anal. Biochem. 33, 132–142 and Itaya, K., and Ui, M. (1966) Clin. Chim. Acta 14, 361) has allowed the development of a manual assay for inorganic phosphate of high simplicity and sensitivity. Total analysis requires only three reagents and i...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 1976-09, Vol.75 (1), p.201-210 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 210 |
|---|---|
| container_issue | 1 |
| container_start_page | 201 |
| container_title | Analytical biochemistry |
| container_volume | 75 |
| creator | Penney, C.L. |
| description | Modification of two assay procedures (Van Belle, H. (1970)
Anal. Biochem.
33, 132–142 and Itaya, K., and Ui, M. (1966)
Clin. Chim. Acta
14, 361) has allowed the development of a manual assay for inorganic phosphate of high simplicity and sensitivity. Total analysis requires only three reagents and is accomplished in less than 5 min, and smaples containing less than 1 μg/ml of inorganic phosphate may be detected. This assay retains a unique principle of the former two, complexation (instead of reduction) of the phosphomolybdate heteropoly complex with an appropriate triphenylmethane dye (malachite green, methyl green). Use of detergents has been eliminated and some further properties of the dyes, the assay, and the latter's applicability to a coupled enzyme system for phosphomonoester and phosphodiester analysis are discussed. Consideration is also given to the associated phenomena of transphosphorylation. (Dayan, J., and Wilson, I. B. (1964)
Biochim. Biophys. Acta
81, 620–623). |
| doi_str_mv | 10.1016/0003-2697(76)90071-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_83471880</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0003269776900713</els_id><sourcerecordid>83471880</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-953f0032b3642b0d0e57b05658e50cc3976fde5087a02af0fb9bfdd46706618c3</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS3EqxT-oIusEEgExnFix5tKVcVLqsQG1pbjjKlRXtgpUv-elFRdspqR5t47M4eQGYV7CpQ_AACLEy7FjeC3EkDQmB2RCQXJY2Agj8nkIDknFyF8AVCaZvyMnEqe0CSfkLtFFFzdVRjVzvg21iHobWRbH7mm9Z-6cSbq1m3o1rrHS3JidRXwal-n5OPp8X35Eq_enl-Xi1VsWMb7WGbMDnuTgvE0KaAEzEQBGc9yzMAYJgW35dDmQkOiLdhCFrYsUy6Ac5obNiXXY27n2-8Nhl7VLhisKt1guwkqZ6mgeQ6DMB2Fw-kheLSq867WfqsoqB0jtQOgdgCU4OqPkWKDbbbP3xQ1lgfTCGUYz8cxDj_-OPQqGIeNwdJ5NL0qW_d__i_0QHOy</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83471880</pqid></control><display><type>article</type><title>A simple micro-assay for inorganic phosphate</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Penney, C.L.</creator><creatorcontrib>Penney, C.L.</creatorcontrib><description>Modification of two assay procedures (Van Belle, H. (1970)
Anal. Biochem.
33, 132–142 and Itaya, K., and Ui, M. (1966)
Clin. Chim. Acta
14, 361) has allowed the development of a manual assay for inorganic phosphate of high simplicity and sensitivity. Total analysis requires only three reagents and is accomplished in less than 5 min, and smaples containing less than 1 μg/ml of inorganic phosphate may be detected. This assay retains a unique principle of the former two, complexation (instead of reduction) of the phosphomolybdate heteropoly complex with an appropriate triphenylmethane dye (malachite green, methyl green). Use of detergents has been eliminated and some further properties of the dyes, the assay, and the latter's applicability to a coupled enzyme system for phosphomonoester and phosphodiester analysis are discussed. Consideration is also given to the associated phenomena of transphosphorylation. (Dayan, J., and Wilson, I. B. (1964)
Biochim. Biophys. Acta
81, 620–623).</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(76)90071-3</identifier><identifier>PMID: 962128</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alkaline Phosphatase - metabolism ; Drug Stability ; Indicators and Reagents ; Methods ; Microchemistry ; Phosphates - analysis ; Spectrophotometry - methods</subject><ispartof>Analytical biochemistry, 1976-09, Vol.75 (1), p.201-210</ispartof><rights>1976</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-953f0032b3642b0d0e57b05658e50cc3976fde5087a02af0fb9bfdd46706618c3</citedby><cites>FETCH-LOGICAL-c356t-953f0032b3642b0d0e57b05658e50cc3976fde5087a02af0fb9bfdd46706618c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(76)90071-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/962128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Penney, C.L.</creatorcontrib><title>A simple micro-assay for inorganic phosphate</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Modification of two assay procedures (Van Belle, H. (1970)
Anal. Biochem.
33, 132–142 and Itaya, K., and Ui, M. (1966)
Clin. Chim. Acta
14, 361) has allowed the development of a manual assay for inorganic phosphate of high simplicity and sensitivity. Total analysis requires only three reagents and is accomplished in less than 5 min, and smaples containing less than 1 μg/ml of inorganic phosphate may be detected. This assay retains a unique principle of the former two, complexation (instead of reduction) of the phosphomolybdate heteropoly complex with an appropriate triphenylmethane dye (malachite green, methyl green). Use of detergents has been eliminated and some further properties of the dyes, the assay, and the latter's applicability to a coupled enzyme system for phosphomonoester and phosphodiester analysis are discussed. Consideration is also given to the associated phenomena of transphosphorylation. (Dayan, J., and Wilson, I. B. (1964)
Biochim. Biophys. Acta
81, 620–623).</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Drug Stability</subject><subject>Indicators and Reagents</subject><subject>Methods</subject><subject>Microchemistry</subject><subject>Phosphates - analysis</subject><subject>Spectrophotometry - methods</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS3EqxT-oIusEEgExnFix5tKVcVLqsQG1pbjjKlRXtgpUv-elFRdspqR5t47M4eQGYV7CpQ_AACLEy7FjeC3EkDQmB2RCQXJY2Agj8nkIDknFyF8AVCaZvyMnEqe0CSfkLtFFFzdVRjVzvg21iHobWRbH7mm9Z-6cSbq1m3o1rrHS3JidRXwal-n5OPp8X35Eq_enl-Xi1VsWMb7WGbMDnuTgvE0KaAEzEQBGc9yzMAYJgW35dDmQkOiLdhCFrYsUy6Ac5obNiXXY27n2-8Nhl7VLhisKt1guwkqZ6mgeQ6DMB2Fw-kheLSq867WfqsoqB0jtQOgdgCU4OqPkWKDbbbP3xQ1lgfTCGUYz8cxDj_-OPQqGIeNwdJ5NL0qW_d__i_0QHOy</recordid><startdate>197609</startdate><enddate>197609</enddate><creator>Penney, C.L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197609</creationdate><title>A simple micro-assay for inorganic phosphate</title><author>Penney, C.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-953f0032b3642b0d0e57b05658e50cc3976fde5087a02af0fb9bfdd46706618c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Drug Stability</topic><topic>Indicators and Reagents</topic><topic>Methods</topic><topic>Microchemistry</topic><topic>Phosphates - analysis</topic><topic>Spectrophotometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Penney, C.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Penney, C.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple micro-assay for inorganic phosphate</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1976-09</date><risdate>1976</risdate><volume>75</volume><issue>1</issue><spage>201</spage><epage>210</epage><pages>201-210</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Modification of two assay procedures (Van Belle, H. (1970)
Anal. Biochem.
33, 132–142 and Itaya, K., and Ui, M. (1966)
Clin. Chim. Acta
14, 361) has allowed the development of a manual assay for inorganic phosphate of high simplicity and sensitivity. Total analysis requires only three reagents and is accomplished in less than 5 min, and smaples containing less than 1 μg/ml of inorganic phosphate may be detected. This assay retains a unique principle of the former two, complexation (instead of reduction) of the phosphomolybdate heteropoly complex with an appropriate triphenylmethane dye (malachite green, methyl green). Use of detergents has been eliminated and some further properties of the dyes, the assay, and the latter's applicability to a coupled enzyme system for phosphomonoester and phosphodiester analysis are discussed. Consideration is also given to the associated phenomena of transphosphorylation. (Dayan, J., and Wilson, I. B. (1964)
Biochim. Biophys. Acta
81, 620–623).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>962128</pmid><doi>10.1016/0003-2697(76)90071-3</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1976-09, Vol.75 (1), p.201-210 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83471880 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alkaline Phosphatase - metabolism Drug Stability Indicators and Reagents Methods Microchemistry Phosphates - analysis Spectrophotometry - methods |
| title | A simple micro-assay for inorganic phosphate |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T01%3A29%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20simple%20micro-assay%20for%20inorganic%20phosphate&rft.jtitle=Analytical%20biochemistry&rft.au=Penney,%20C.L.&rft.date=1976-09&rft.volume=75&rft.issue=1&rft.spage=201&rft.epage=210&rft.pages=201-210&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/0003-2697(76)90071-3&rft_dat=%3Cproquest_cross%3E83471880%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=83471880&rft_id=info:pmid/962128&rft_els_id=0003269776900713&rfr_iscdi=true |