A RAPID METHOD FOR ASSAYING ENZYMES WHOSE SUBSTRATES AND PRODUCTS DIFFER BY CHARGE. APPLICATION TO BRAIN l‐GLUTAMATE DECARBOXYLASE

A RAPID METHOD FOR ASSAYING ENZYMES WHOSE SUBSTRATES AND PRODUCTS DIFFER BY CHARGE. APPLICATION TO BRAIN l‐GLUTAMATE DECARBOXYLASE

— A simple, rapid, sensitive and economical assay technique has been developed for any enzyme whose substrate and product differ by charge. It is based on a combination of rapid vacuum filtration with an ion exchange resin. The technique has been successfully applied to brain glutamate decarboxylase...

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Veröffentlicht in:Journal of neurochemistry 1976-07, Vol.27 (1), p.83-86
Hauptverfasser: Chude, Obi, Wu, Jang‐Yen
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Brain - enzymology
Carboxy-Lyases - analysis
Chromatography, Ion Exchange
Electrophoresis, Paper
Glutamate Decarboxylase - analysis
Methods
Mice
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container_end_page 86
container_issue 1
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container_title Journal of neurochemistry
container_volume 27
creator Chude, Obi
Wu, Jang‐Yen
description — A simple, rapid, sensitive and economical assay technique has been developed for any enzyme whose substrate and product differ by charge. It is based on a combination of rapid vacuum filtration with an ion exchange resin. The technique has been successfully applied to brain glutamate decarboxylase.
doi_str_mv 10.1111/j.1471-4159.1976.tb01547.x
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source MEDLINE; Access via Wiley Online Library
subjects Animals
Brain - enzymology
Carboxy-Lyases - analysis
Chromatography, Ion Exchange
Electrophoresis, Paper
Glutamate Decarboxylase - analysis
Methods
Mice
title A RAPID METHOD FOR ASSAYING ENZYMES WHOSE SUBSTRATES AND PRODUCTS DIFFER BY CHARGE. APPLICATION TO BRAIN l‐GLUTAMATE DECARBOXYLASE
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