A RAPID METHOD FOR ASSAYING ENZYMES WHOSE SUBSTRATES AND PRODUCTS DIFFER BY CHARGE. APPLICATION TO BRAIN l‐GLUTAMATE DECARBOXYLASE

— A simple, rapid, sensitive and economical assay technique has been developed for any enzyme whose substrate and product differ by charge. It is based on a combination of rapid vacuum filtration with an ion exchange resin. The technique has been successfully applied to brain glutamate decarboxylase...

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Veröffentlicht in:	Journal of neurochemistry 1976-07, Vol.27 (1), p.83-86
Hauptverfasser:	Chude, Obi, Wu, Jang‐Yen
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain - enzymology Carboxy-Lyases - analysis Chromatography, Ion Exchange Electrophoresis, Paper Glutamate Decarboxylase - analysis Methods Mice
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	— A simple, rapid, sensitive and economical assay technique has been developed for any enzyme whose substrate and product differ by charge. It is based on a combination of rapid vacuum filtration with an ion exchange resin. The technique has been successfully applied to brain glutamate decarboxylase.
ISSN:	0022-3042 1471-4159
DOI:	10.1111/j.1471-4159.1976.tb01547.x