Ruthenium red as a carrier of electrons between external NADH and cytochrome C in rat liver mitochondria
Exogenous NADH is oxidized by rat liver mitochondria when ruthenium red (2μM) is present in the medium. The oxidation is insensitive to rotenone and antimycin A, but sensitive to KCN, and is markedly reduced in a saline medium or in the presence of divalent cations. The same effect of ruthenium red...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1976-04, Vol.69 (3), p.812-815 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Schwerzmann, Klaus Gazzotti, Paolo Carafoli, Ernesto |
| description | Exogenous NADH is oxidized by rat liver mitochondria when ruthenium red (2μM) is present in the medium. The oxidation is insensitive to rotenone and antimycin A, but sensitive to KCN, and is markedly reduced in a saline medium or in the presence of divalent cations. The same effect of ruthenium red can be seen in mitochondria depleted of the outer membrane. This suggests that ruthenium red acts as a mediator of electrons between NADH and cytochrome c. This is also supported by the observation that ruthenium red can replace N,N,N
1,N
1-tetramethyl-p-phenylenediamine (TMPD) in the oxidation of ascorbate by mitochondria. The effect of ruthenium red is abolished by LaCl
3. |
| doi_str_mv | 10.1016/0006-291X(76)90947-5 |
| format | Article |
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1,N
1-tetramethyl-p-phenylenediamine (TMPD) in the oxidation of ascorbate by mitochondria. The effect of ruthenium red is abolished by LaCl
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1,N
1-tetramethyl-p-phenylenediamine (TMPD) in the oxidation of ascorbate by mitochondria. The effect of ruthenium red is abolished by LaCl
3.</description><subject>Animals</subject><subject>Biological Transport, Active</subject><subject>Cytochrome c Group - metabolism</subject><subject>Lanthanum - pharmacology</subject><subject>Mitochondria, Liver - drug effects</subject><subject>Mitochondria, Liver - metabolism</subject><subject>NAD - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Rats</subject><subject>Ruthenium - metabolism</subject><subject>Ruthenium Red - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAURa2KtgzT_gMWXiFYpNiJ48QbJDR8VRpRqWql7qwX-0XjKrGpnUD593gYBDtWb3HuvdI7hBxy9o0zLk8ZY7IoFf9z3MgTxZRoivoDWXCmWFFyJvbI4jWyTw5S-ssY50Kqz-QTb9qKNwuy-TlPG_RuHmlESyFRoAZidBhp6CkOaKYYfKIdTg-InuL_CaOHgd6eX9xQ8JaaxymYTQwj0hV1nkaY6ODu88DotiR4Gx18IR97GBJ-fblL8vvq8tfqplj_uP6-Ol8XpqrlVACgtLUwoulszyrZtg1YaSwHMMZmIm2jemSsV5UVOV0bBlC2XWdUqcquWpKj3e5dDP9mTJMeXTI4DOAxzEm3lSjLloscFLugiSGliL2-i26E-Kg501u_eitPb-XpRupnv7rOtcOX_bkb0b6VnoVmfLbDmH-8zxZ1Mg69QetiVqltcO_vPwEUDYwI</recordid><startdate>19760405</startdate><enddate>19760405</enddate><creator>Schwerzmann, Klaus</creator><creator>Gazzotti, Paolo</creator><creator>Carafoli, Ernesto</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19760405</creationdate><title>Ruthenium red as a carrier of electrons between external NADH and cytochrome C in rat liver mitochondria</title><author>Schwerzmann, Klaus ; Gazzotti, Paolo ; Carafoli, Ernesto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-aae6d54c47bdf036887ad6cd1aaccdd546d79fe00f93d4aae5c0aa28bbc9292b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Biological Transport, Active</topic><topic>Cytochrome c Group - metabolism</topic><topic>Lanthanum - pharmacology</topic><topic>Mitochondria, Liver - drug effects</topic><topic>Mitochondria, Liver - metabolism</topic><topic>NAD - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Rats</topic><topic>Ruthenium - metabolism</topic><topic>Ruthenium Red - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schwerzmann, Klaus</creatorcontrib><creatorcontrib>Gazzotti, Paolo</creatorcontrib><creatorcontrib>Carafoli, Ernesto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schwerzmann, Klaus</au><au>Gazzotti, Paolo</au><au>Carafoli, Ernesto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ruthenium red as a carrier of electrons between external NADH and cytochrome C in rat liver mitochondria</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1976-04-05</date><risdate>1976</risdate><volume>69</volume><issue>3</issue><spage>812</spage><epage>815</epage><pages>812-815</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Exogenous NADH is oxidized by rat liver mitochondria when ruthenium red (2μM) is present in the medium. The oxidation is insensitive to rotenone and antimycin A, but sensitive to KCN, and is markedly reduced in a saline medium or in the presence of divalent cations. The same effect of ruthenium red can be seen in mitochondria depleted of the outer membrane. This suggests that ruthenium red acts as a mediator of electrons between NADH and cytochrome c. This is also supported by the observation that ruthenium red can replace N,N,N
1,N
1-tetramethyl-p-phenylenediamine (TMPD) in the oxidation of ascorbate by mitochondria. The effect of ruthenium red is abolished by LaCl
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| ispartof | Biochemical and biophysical research communications, 1976-04, Vol.69 (3), p.812-815 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological Transport, Active Cytochrome c Group - metabolism Lanthanum - pharmacology Mitochondria, Liver - drug effects Mitochondria, Liver - metabolism NAD - metabolism Oxidation-Reduction Rats Ruthenium - metabolism Ruthenium Red - metabolism |
| title | Ruthenium red as a carrier of electrons between external NADH and cytochrome C in rat liver mitochondria |
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