EXPERIMENTAL PANENCEPHALITIS INDUCED IN SUCKLING MICE BY PARAINFLUENZA TYPE 1 (6/94) VIRUS: II. VIROLOGIC STUDIES
6/94 virus, a parainfluenza type 1 isolate from multiple sclerosis brain tissue, produced a chronic panencephalitis when inoculated intracerebrally into suckling ICR mice. Immunofluorescent staining revealed 6/94 viral antigen in ependyma, meninges, choroid plexus, and perivascular parenchy-mal site...
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| Veröffentlicht in: | Journal of neuropathology and experimental neurology 1976-05, Vol.35 (3), p.259-270 |
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| container_title | Journal of neuropathology and experimental neurology |
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| creator | GILDEN, DONALD H WROBLEWSKA, ZOFIA CHESLER, MARILYN WELLISH, MARY C LIEF, FLORENCE S WOLINSKY, JERRY S RORKE, LUCY BALIAN |
| description | 6/94 virus, a parainfluenza type 1 isolate from multiple sclerosis brain tissue, produced a chronic panencephalitis when inoculated intracerebrally into suckling ICR mice. Immunofluorescent staining revealed 6/94 viral antigen in ependyma, meninges, choroid plexus, and perivascular parenchy-mal sites from day 3 to 128 days after infection. Hemadsorption-neutralizing antibody was first detected between 20–25 days after infection and remained at high titers for 7 months. Using embryonated chicken eggs, virus was recovered from mouse brains for only 8 days, but could be recovered from brains grown in vitro as explants for 37 days after infection. In cell lines established from explanted brain tissue, immunofluorescence was the most sensitive indicator of virus presence, although infectious virus was not produced. Fusion of these mouse brain cells with human (WI38) indicator cells was the most effective means of rescuing 6/94 virus. |
| doi_str_mv | 10.1097/00005072-197605000-00004 |
| format | Article |
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VIROLOGIC STUDIES</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>Journals@Ovid Complete</source><creator>GILDEN, DONALD H ; WROBLEWSKA, ZOFIA ; CHESLER, MARILYN ; WELLISH, MARY C ; LIEF, FLORENCE S ; WOLINSKY, JERRY S ; RORKE, LUCY BALIAN</creator><creatorcontrib>GILDEN, DONALD H ; WROBLEWSKA, ZOFIA ; CHESLER, MARILYN ; WELLISH, MARY C ; LIEF, FLORENCE S ; WOLINSKY, JERRY S ; RORKE, LUCY BALIAN</creatorcontrib><description>6/94 virus, a parainfluenza type 1 isolate from multiple sclerosis brain tissue, produced a chronic panencephalitis when inoculated intracerebrally into suckling ICR mice. Immunofluorescent staining revealed 6/94 viral antigen in ependyma, meninges, choroid plexus, and perivascular parenchy-mal sites from day 3 to 128 days after infection. Hemadsorption-neutralizing antibody was first detected between 20–25 days after infection and remained at high titers for 7 months. Using embryonated chicken eggs, virus was recovered from mouse brains for only 8 days, but could be recovered from brains grown in vitro as explants for 37 days after infection. In cell lines established from explanted brain tissue, immunofluorescence was the most sensitive indicator of virus presence, although infectious virus was not produced. Fusion of these mouse brain cells with human (WI38) indicator cells was the most effective means of rescuing 6/94 virus.</description><identifier>ISSN: 0022-3069</identifier><identifier>EISSN: 1554-6578</identifier><identifier>DOI: 10.1097/00005072-197605000-00004</identifier><identifier>PMID: 178834</identifier><language>eng</language><publisher>England: American Association of Neuropathologists, Inc</publisher><subject>Animals ; Antigens, Viral - analysis ; Cells, Cultured ; Cerebral Cortex - microbiology ; Encephalitis - immunology ; Encephalitis - microbiology ; Mice ; Multiple Sclerosis - microbiology ; Parainfluenza Virus 1, Human - immunology ; Parainfluenza Virus 1, Human - isolation & purification ; Time Factors</subject><ispartof>Journal of neuropathology and experimental neurology, 1976-05, Vol.35 (3), p.259-270</ispartof><rights>1976 American Association of Neuropathologists, Inc</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/178834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GILDEN, DONALD H</creatorcontrib><creatorcontrib>WROBLEWSKA, ZOFIA</creatorcontrib><creatorcontrib>CHESLER, MARILYN</creatorcontrib><creatorcontrib>WELLISH, MARY C</creatorcontrib><creatorcontrib>LIEF, FLORENCE S</creatorcontrib><creatorcontrib>WOLINSKY, JERRY S</creatorcontrib><creatorcontrib>RORKE, LUCY BALIAN</creatorcontrib><title>EXPERIMENTAL PANENCEPHALITIS INDUCED IN SUCKLING MICE BY PARAINFLUENZA TYPE 1 (6/94) VIRUS: II. VIROLOGIC STUDIES</title><title>Journal of neuropathology and experimental neurology</title><addtitle>J Neuropathol Exp Neurol</addtitle><description>6/94 virus, a parainfluenza type 1 isolate from multiple sclerosis brain tissue, produced a chronic panencephalitis when inoculated intracerebrally into suckling ICR mice. Immunofluorescent staining revealed 6/94 viral antigen in ependyma, meninges, choroid plexus, and perivascular parenchy-mal sites from day 3 to 128 days after infection. Hemadsorption-neutralizing antibody was first detected between 20–25 days after infection and remained at high titers for 7 months. Using embryonated chicken eggs, virus was recovered from mouse brains for only 8 days, but could be recovered from brains grown in vitro as explants for 37 days after infection. In cell lines established from explanted brain tissue, immunofluorescence was the most sensitive indicator of virus presence, although infectious virus was not produced. Fusion of these mouse brain cells with human (WI38) indicator cells was the most effective means of rescuing 6/94 virus.</description><subject>Animals</subject><subject>Antigens, Viral - analysis</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - microbiology</subject><subject>Encephalitis - immunology</subject><subject>Encephalitis - microbiology</subject><subject>Mice</subject><subject>Multiple Sclerosis - microbiology</subject><subject>Parainfluenza Virus 1, Human - immunology</subject><subject>Parainfluenza Virus 1, Human - isolation & purification</subject><subject>Time Factors</subject><issn>0022-3069</issn><issn>1554-6578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kVFP2zAQx61pjBW2b8CDn6bxEDg7jh3vLUsNWAuhapJp7MVKU1uwpRTiVohvj9uy8cS93Onud3e6_yGECZwQkOIUgiUgaESk4CECiDYp9g6NSJKwiCcifY9GAJRGMXD5ER14_ycQEiTbRx-ISNOYjdCD-jVRU32pyjor8CQrVZmryUVW6FpXWJfjJlfj4HHV5D8KXZ7jS50r_P06sNNMl2dFo8rfGa6vJwoT_JWfSnaMf-ppU33DWp9swqvi6lznuKqbsVbVJ7Tn2t7bzy_-EDVnqs4voi2VFVFHGGdROIunHaMxyBnMBRUzsJwLSyhz7UxIKVpwztIOnIS5dNIC4c7RVkqbQNfGh-jLbu79sHxYW78yi1vf2b5v7-xy7U04n5KUygCmO7Ablt4P1pn74XbRDk-GgNmIbf6Jbf6LvU2x0Hr0smM9W9j5a-NW3VBmu_Ljsl_Zwf_t1492MDe27Vc35q0Xxs9ML367</recordid><startdate>197605</startdate><enddate>197605</enddate><creator>GILDEN, DONALD H</creator><creator>WROBLEWSKA, ZOFIA</creator><creator>CHESLER, MARILYN</creator><creator>WELLISH, MARY C</creator><creator>LIEF, FLORENCE S</creator><creator>WOLINSKY, JERRY S</creator><creator>RORKE, LUCY BALIAN</creator><general>American Association of Neuropathologists, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197605</creationdate><title>EXPERIMENTAL PANENCEPHALITIS INDUCED IN SUCKLING MICE BY PARAINFLUENZA TYPE 1 (6/94) VIRUS: II. VIROLOGIC STUDIES</title><author>GILDEN, DONALD H ; WROBLEWSKA, ZOFIA ; CHESLER, MARILYN ; WELLISH, MARY C ; LIEF, FLORENCE S ; WOLINSKY, JERRY S ; RORKE, LUCY BALIAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1464-97668c42309b0d727b0e667e124fab7997a0ffe2c0f90d9f9e016ff2a99e50ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Antigens, Viral - analysis</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - microbiology</topic><topic>Encephalitis - immunology</topic><topic>Encephalitis - microbiology</topic><topic>Mice</topic><topic>Multiple Sclerosis - microbiology</topic><topic>Parainfluenza Virus 1, Human - immunology</topic><topic>Parainfluenza Virus 1, Human - isolation & purification</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GILDEN, DONALD H</creatorcontrib><creatorcontrib>WROBLEWSKA, ZOFIA</creatorcontrib><creatorcontrib>CHESLER, MARILYN</creatorcontrib><creatorcontrib>WELLISH, MARY C</creatorcontrib><creatorcontrib>LIEF, FLORENCE S</creatorcontrib><creatorcontrib>WOLINSKY, JERRY S</creatorcontrib><creatorcontrib>RORKE, LUCY BALIAN</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuropathology and experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GILDEN, DONALD H</au><au>WROBLEWSKA, ZOFIA</au><au>CHESLER, MARILYN</au><au>WELLISH, MARY C</au><au>LIEF, FLORENCE S</au><au>WOLINSKY, JERRY S</au><au>RORKE, LUCY BALIAN</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EXPERIMENTAL PANENCEPHALITIS INDUCED IN SUCKLING MICE BY PARAINFLUENZA TYPE 1 (6/94) VIRUS: II. VIROLOGIC STUDIES</atitle><jtitle>Journal of neuropathology and experimental neurology</jtitle><addtitle>J Neuropathol Exp Neurol</addtitle><date>1976-05</date><risdate>1976</risdate><volume>35</volume><issue>3</issue><spage>259</spage><epage>270</epage><pages>259-270</pages><issn>0022-3069</issn><eissn>1554-6578</eissn><abstract>6/94 virus, a parainfluenza type 1 isolate from multiple sclerosis brain tissue, produced a chronic panencephalitis when inoculated intracerebrally into suckling ICR mice. Immunofluorescent staining revealed 6/94 viral antigen in ependyma, meninges, choroid plexus, and perivascular parenchy-mal sites from day 3 to 128 days after infection. Hemadsorption-neutralizing antibody was first detected between 20–25 days after infection and remained at high titers for 7 months. Using embryonated chicken eggs, virus was recovered from mouse brains for only 8 days, but could be recovered from brains grown in vitro as explants for 37 days after infection. In cell lines established from explanted brain tissue, immunofluorescence was the most sensitive indicator of virus presence, although infectious virus was not produced. Fusion of these mouse brain cells with human (WI38) indicator cells was the most effective means of rescuing 6/94 virus.</abstract><cop>England</cop><pub>American Association of Neuropathologists, Inc</pub><pmid>178834</pmid><doi>10.1097/00005072-197605000-00004</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0022-3069 |
| ispartof | Journal of neuropathology and experimental neurology, 1976-05, Vol.35 (3), p.259-270 |
| issn | 0022-3069 1554-6578 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; Journals@Ovid Complete |
| subjects | Animals Antigens, Viral - analysis Cells, Cultured Cerebral Cortex - microbiology Encephalitis - immunology Encephalitis - microbiology Mice Multiple Sclerosis - microbiology Parainfluenza Virus 1, Human - immunology Parainfluenza Virus 1, Human - isolation & purification Time Factors |
| title | EXPERIMENTAL PANENCEPHALITIS INDUCED IN SUCKLING MICE BY PARAINFLUENZA TYPE 1 (6/94) VIRUS: II. VIROLOGIC STUDIES |
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