α-FETOPROTEIN AND ALBUMIN PRODUCED BY SUBCLONAL CELL POPULATION OF THE ASCITES HEPATOMA AH-66 IN VITRO

Subclonal cell populations were prepared from a clonal line of the rat ascites hepatoma AH-66 in vitro, using the agar plate culture method. α-Fetoprotein and albumin concentrations in culture media of these subclones were determined by 125I-radioimmunoassay and single radial immunodiffusion method,...

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Veröffentlicht in:	GANN Japanese Journal of Cancer Research 1976/02/29, Vol.67(1), pp.131-135
Hauptverfasser:	ISAKA, Hidehiko, UMEHARA, Sumiko, YOSHII, Hirooki, TSUKADA, Yutaka, HIRAI, Hidematsu
Format:	Artikel
Sprache:	eng
Schlagworte:	AH-66 in culture Albumin Albumins - biosynthesis alpha-Fetoproteins - biosynthesis Carcinoma, Hepatocellular - metabolism Clone Cells Fetal Proteins - biosynthesis Liver Neoplasms Neoplasms, Experimental Rat Subclone α-Fetoprotein
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container_title	GANN Japanese Journal of Cancer Research
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creator	ISAKA, Hidehiko UMEHARA, Sumiko YOSHII, Hirooki TSUKADA, Yutaka HIRAI, Hidematsu
description	Subclonal cell populations were prepared from a clonal line of the rat ascites hepatoma AH-66 in vitro, using the agar plate culture method. α-Fetoprotein and albumin concentrations in culture media of these subclones were determined by 125I-radioimmunoassay and single radial immunodiffusion method, respectively. Results demonstrated that the AH-66 clone was a complex of cells with varying producibility of α-fetoprotein and albumin. All the subclones showed varied and distinct production of α-fetoprotein, but not all the subclones produced detectable levels of albumin.
doi_str_mv	10.20772/cancersci1959.67.1_131
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source	J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects	AH-66 in culture Albumin Albumins - biosynthesis alpha-Fetoproteins - biosynthesis Carcinoma, Hepatocellular - metabolism Clone Cells Fetal Proteins - biosynthesis Liver Neoplasms Neoplasms, Experimental Rat Subclone α-Fetoprotein
title	α-FETOPROTEIN AND ALBUMIN PRODUCED BY SUBCLONAL CELL POPULATION OF THE ASCITES HEPATOMA AH-66 IN VITRO
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