Ischemic Areas in Perfused Rat Hearts: Measurement by NADH Fluorescence Photography
Fluorescence emission of reduced nicotinamide adenine dinucleotide (NADH) from the surface of perfused rat hearts was photographed to provide a two-dimensional recording of NADH levels. Sodium Amytal inhibition of NADH oxidation resulted in a homogeneous increase in NADH fluorescence, while lowering...
Gespeichert in:
| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1976-09, Vol.193 (4256), p.909-910 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 910 |
|---|---|
| container_issue | 4256 |
| container_start_page | 909 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 193 |
| creator | Barlow, Clyde H. Chance, Britton |
| description | Fluorescence emission of reduced nicotinamide adenine dinucleotide (NADH) from the surface of perfused rat hearts was photographed to provide a two-dimensional recording of NADH levels. Sodium Amytal inhibition of NADH oxidation resulted in a homogeneous increase in NADH fluorescence, while lowering the perfusion pressure from 55 to 10 torr caused a heterogeneous increase in NADH fluorescence, reflecting the heterogeneous oxygen delivery at this low pressure. Local ischemia resulted in a well-defined region of high NADH fluorescence that corresponded to the region of ischemic insult. The sharp transition between the ischemic and normoxic areas demonstrated that the hypoxic interface separating the two areas must be quite small. |
| doi_str_mv | 10.1126/science.181843 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_83415536</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>1742286</jstor_id><sourcerecordid>1742286</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-d0af3cd3c3cac1ea416ab1a09a8ba8374972ec75b2ce847bb755300568d0e393</originalsourceid><addsrcrecordid>eNpdkD1PwzAQhi3EVyisTAye2FLOsZM4bFWhtFKBCrpHjnOhqfJRbGfovydVKlViuuF53rvTS8g9gzFjQfRkdYmNxjGTTAp-RjwGSegnAfBz4gHwyJcQh9fkxtotQM8SfkUuB9sj3wurN1iXmk4MKkvLhq7QFJ3FnH4pR-eojLPP9L2HncEaG0ezPf2YvMzprOpag1YfztPVpnXtj1G7zf6WXBSqsnh3nCOynr2up3N_-fm2mE6WvhYQOT8HVXCdc8210gyVYJHKmIJEyUxJHoskDlDHYRZolCLOsjgMOUAYyRyQJ3xEHoe1O9P-dmhdWpf9M1WlGmw7m0ouWJ-IenE8iNq01hos0p0pa2X2KYP00GF67DAdWukDD8fNXVZjftL_4a11rTnRWASBjPgf_p53cQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83415536</pqid></control><display><type>article</type><title>Ischemic Areas in Perfused Rat Hearts: Measurement by NADH Fluorescence Photography</title><source>MEDLINE</source><source>Science Online_科学在线</source><source>JSTOR</source><creator>Barlow, Clyde H. ; Chance, Britton</creator><creatorcontrib>Barlow, Clyde H. ; Chance, Britton</creatorcontrib><description>Fluorescence emission of reduced nicotinamide adenine dinucleotide (NADH) from the surface of perfused rat hearts was photographed to provide a two-dimensional recording of NADH levels. Sodium Amytal inhibition of NADH oxidation resulted in a homogeneous increase in NADH fluorescence, while lowering the perfusion pressure from 55 to 10 torr caused a heterogeneous increase in NADH fluorescence, reflecting the heterogeneous oxygen delivery at this low pressure. Local ischemia resulted in a well-defined region of high NADH fluorescence that corresponded to the region of ischemic insult. The sharp transition between the ischemic and normoxic areas demonstrated that the hypoxic interface separating the two areas must be quite small.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.181843</identifier><identifier>PMID: 181843</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Animals ; Coronary Disease - diagnosis ; Coronary Disease - metabolism ; Coronary Disease - pathology ; Cultured cells ; Fluorescence ; Heart ; Heart ventricles ; Hepatocytes ; Male ; Metabolism ; NAD - metabolism ; Oxygen ; Perfusion ; Photofluorography ; Photographic lenses ; Rats ; Sodium</subject><ispartof>Science (American Association for the Advancement of Science), 1976-09, Vol.193 (4256), p.909-910</ispartof><rights>Copyright 1976 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-d0af3cd3c3cac1ea416ab1a09a8ba8374972ec75b2ce847bb755300568d0e393</citedby><cites>FETCH-LOGICAL-c406t-d0af3cd3c3cac1ea416ab1a09a8ba8374972ec75b2ce847bb755300568d0e393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1742286$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1742286$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/181843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barlow, Clyde H.</creatorcontrib><creatorcontrib>Chance, Britton</creatorcontrib><title>Ischemic Areas in Perfused Rat Hearts: Measurement by NADH Fluorescence Photography</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Fluorescence emission of reduced nicotinamide adenine dinucleotide (NADH) from the surface of perfused rat hearts was photographed to provide a two-dimensional recording of NADH levels. Sodium Amytal inhibition of NADH oxidation resulted in a homogeneous increase in NADH fluorescence, while lowering the perfusion pressure from 55 to 10 torr caused a heterogeneous increase in NADH fluorescence, reflecting the heterogeneous oxygen delivery at this low pressure. Local ischemia resulted in a well-defined region of high NADH fluorescence that corresponded to the region of ischemic insult. The sharp transition between the ischemic and normoxic areas demonstrated that the hypoxic interface separating the two areas must be quite small.</description><subject>Animals</subject><subject>Coronary Disease - diagnosis</subject><subject>Coronary Disease - metabolism</subject><subject>Coronary Disease - pathology</subject><subject>Cultured cells</subject><subject>Fluorescence</subject><subject>Heart</subject><subject>Heart ventricles</subject><subject>Hepatocytes</subject><subject>Male</subject><subject>Metabolism</subject><subject>NAD - metabolism</subject><subject>Oxygen</subject><subject>Perfusion</subject><subject>Photofluorography</subject><subject>Photographic lenses</subject><subject>Rats</subject><subject>Sodium</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkD1PwzAQhi3EVyisTAye2FLOsZM4bFWhtFKBCrpHjnOhqfJRbGfovydVKlViuuF53rvTS8g9gzFjQfRkdYmNxjGTTAp-RjwGSegnAfBz4gHwyJcQh9fkxtotQM8SfkUuB9sj3wurN1iXmk4MKkvLhq7QFJ3FnH4pR-eojLPP9L2HncEaG0ezPf2YvMzprOpag1YfztPVpnXtj1G7zf6WXBSqsnh3nCOynr2up3N_-fm2mE6WvhYQOT8HVXCdc8210gyVYJHKmIJEyUxJHoskDlDHYRZolCLOsjgMOUAYyRyQJ3xEHoe1O9P-dmhdWpf9M1WlGmw7m0ouWJ-IenE8iNq01hos0p0pa2X2KYP00GF67DAdWukDD8fNXVZjftL_4a11rTnRWASBjPgf_p53cQ</recordid><startdate>19760903</startdate><enddate>19760903</enddate><creator>Barlow, Clyde H.</creator><creator>Chance, Britton</creator><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19760903</creationdate><title>Ischemic Areas in Perfused Rat Hearts: Measurement by NADH Fluorescence Photography</title><author>Barlow, Clyde H. ; Chance, Britton</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-d0af3cd3c3cac1ea416ab1a09a8ba8374972ec75b2ce847bb755300568d0e393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Coronary Disease - diagnosis</topic><topic>Coronary Disease - metabolism</topic><topic>Coronary Disease - pathology</topic><topic>Cultured cells</topic><topic>Fluorescence</topic><topic>Heart</topic><topic>Heart ventricles</topic><topic>Hepatocytes</topic><topic>Male</topic><topic>Metabolism</topic><topic>NAD - metabolism</topic><topic>Oxygen</topic><topic>Perfusion</topic><topic>Photofluorography</topic><topic>Photographic lenses</topic><topic>Rats</topic><topic>Sodium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barlow, Clyde H.</creatorcontrib><creatorcontrib>Chance, Britton</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barlow, Clyde H.</au><au>Chance, Britton</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ischemic Areas in Perfused Rat Hearts: Measurement by NADH Fluorescence Photography</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1976-09-03</date><risdate>1976</risdate><volume>193</volume><issue>4256</issue><spage>909</spage><epage>910</epage><pages>909-910</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Fluorescence emission of reduced nicotinamide adenine dinucleotide (NADH) from the surface of perfused rat hearts was photographed to provide a two-dimensional recording of NADH levels. Sodium Amytal inhibition of NADH oxidation resulted in a homogeneous increase in NADH fluorescence, while lowering the perfusion pressure from 55 to 10 torr caused a heterogeneous increase in NADH fluorescence, reflecting the heterogeneous oxygen delivery at this low pressure. Local ischemia resulted in a well-defined region of high NADH fluorescence that corresponded to the region of ischemic insult. The sharp transition between the ischemic and normoxic areas demonstrated that the hypoxic interface separating the two areas must be quite small.</abstract><cop>United States</cop><pub>American Association for the Advancement of Science</pub><pmid>181843</pmid><doi>10.1126/science.181843</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1976-09, Vol.193 (4256), p.909-910 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83415536 |
| source | MEDLINE; Science Online_科学在线; JSTOR |
| subjects | Animals Coronary Disease - diagnosis Coronary Disease - metabolism Coronary Disease - pathology Cultured cells Fluorescence Heart Heart ventricles Hepatocytes Male Metabolism NAD - metabolism Oxygen Perfusion Photofluorography Photographic lenses Rats Sodium |
| title | Ischemic Areas in Perfused Rat Hearts: Measurement by NADH Fluorescence Photography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T21%3A26%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ischemic%20Areas%20in%20Perfused%20Rat%20Hearts:%20Measurement%20by%20NADH%20Fluorescence%20Photography&rft.jtitle=Science%20(American%20Association%20for%20the%20Advancement%20of%20Science)&rft.au=Barlow,%20Clyde%20H.&rft.date=1976-09-03&rft.volume=193&rft.issue=4256&rft.spage=909&rft.epage=910&rft.pages=909-910&rft.issn=0036-8075&rft.eissn=1095-9203&rft_id=info:doi/10.1126/science.181843&rft_dat=%3Cjstor_proqu%3E1742286%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=83415536&rft_id=info:pmid/181843&rft_jstor_id=1742286&rfr_iscdi=true |