Measurement of 5′-adenylic acid by stimulation of phosphorylase a

A method for measuring 5′-adenylic acid is described which is based on its capacity to stimulate the activity of phosphorylase a in the presence of low levels of glycogen and inorganic P. The method is useful with very low concentrations of AMP (0.02 to 0.5 μ m) and is unaffected by much higher leve...

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Veröffentlicht in:	Analytical biochemistry 1976-02, Vol.70 (2), p.554-562
Hauptverfasser:	Barbehenn, Elizabeth K., Law, Maggie M.-Y., Brown, Joseph G., Lowry, Oliver H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Monophosphate - analysis In Vitro Techniques Indicators and Reagents Ions Liver Glycogen NADP Osmolar Concentration Phosphates Phosphorylases Spectrometry, Fluorescence
Online-Zugang:	Volltext
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