Physical and kinetic properties of homogenous bovine lens aldose reductase

Aldose reductase from calf lens was purified 15,000-fold. The homogeneity of the final preparation was demonstrated by molecular sieve chromatography, analytical ultracentrifugation, sodium dodecyl sulfate gel electrophoresis, Ouchterlony immunodiffusion, and polyacrylamide gel electrophoresis at th...

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Veröffentlicht in:	The Journal of biological chemistry 1976-05, Vol.251 (9), p.2696-2702
Hauptverfasser:	Sheaff, C M, Doughty, C C
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Oxidoreductases - metabolism Aldehyde Reductase - isolation & purification Aldehyde Reductase - metabolism Amino Acids - analysis Animals Cattle Circular Dichroism Immunodiffusion Kinetics Lens, Crystalline - enzymology Mathematics Mercaptoethanol - pharmacology Molecular Weight NADP - pharmacology Protein Conformation Spectrophotometry, Infrared Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
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