In-vitro cultivation of human cytomegalovirus in thyroid epithelial cells
Several strains of human cytomegalovirus including recent isolates, were grown in epithelial cells derived from thyroid tissue. All the strains tested grew in these cultures without pre-treatment of the cells, and no difference in cytopathic effect was detected between strains of genital and non-gen...
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Veröffentlicht in: | Archives of virology 1976-03, Vol.50 (1-2), p.119-124 |
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description | Several strains of human cytomegalovirus including recent isolates, were grown in epithelial cells derived from thyroid tissue. All the strains tested grew in these cultures without pre-treatment of the cells, and no difference in cytopathic effect was detected between strains of genital and non-genital origin. 4 strains of CMV were isolated directly from urine in thyroid cells; however the failure to isolate 6 further strains in these cultures from other specimens may indicate that a higher multiplicity of infection is required to infect epithelial than fibroblast cells. |
doi_str_mv | 10.1007/BF01318006 |
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All the strains tested grew in these cultures without pre-treatment of the cells, and no difference in cytopathic effect was detected between strains of genital and non-genital origin. 4 strains of CMV were isolated directly from urine in thyroid cells; however the failure to isolate 6 further strains in these cultures from other specimens may indicate that a higher multiplicity of infection is required to infect epithelial than fibroblast cells.</description><subject>Cervix Uteri - microbiology</subject><subject>Culture Techniques</subject><subject>Cytomegalovirus - growth & development</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>Cytopathogenic Effect, Viral</subject><subject>Female</subject><subject>Humans</subject><subject>Thyroid Gland - microbiology</subject><subject>Urine - microbiology</subject><subject>Virus Cultivation - methods</subject><subject>Virus Replication</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M1LwzAYBvAgfs3pxbOHnDwI1TfNR9OjDqeDgRc9lzRNXSRtZpIO9t_bucFOL7z8eHh4ELol8EgAiqeXORBKJIA4QRPCaJ7JopSnaAIUWCYFyEt0FeMPwPig_AKdk0KUQkzQYtFnG5uCx3pwyW5Usr7HvsWroVM91tvkO_OtnN_YMERse5xW2-Btg83appVxVjmsjXPxGp21ykVzc7hT9DV__Zy9Z8uPt8XseZlpUoqUUVo3mhSqzuscciEZ50IC521Lgei8bhkpoWCcNrQhoEYoBS8Lzlgjc5ZzOkX3-9x18L-DianqbNw1UL3xQ6wkpUyOfoQPe6iDjzGYtloH26mwrQhUu9mq42wjvjukDnVnmiP934n-AX8zZkk</recordid><startdate>197603</startdate><enddate>197603</enddate><creator>Knowles, W A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197603</creationdate><title>In-vitro cultivation of human cytomegalovirus in thyroid epithelial cells</title><author>Knowles, W A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c196t-33bdc17ab2b2026845568055ff301c2bf41907453d3d10a7ab86597544d824253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Cervix Uteri - microbiology</topic><topic>Culture Techniques</topic><topic>Cytomegalovirus - growth & development</topic><topic>Cytomegalovirus - isolation & purification</topic><topic>Cytopathogenic Effect, Viral</topic><topic>Female</topic><topic>Humans</topic><topic>Thyroid Gland - microbiology</topic><topic>Urine - microbiology</topic><topic>Virus Cultivation - methods</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knowles, W A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knowles, W A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-vitro cultivation of human cytomegalovirus in thyroid epithelial cells</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>1976-03</date><risdate>1976</risdate><volume>50</volume><issue>1-2</issue><spage>119</spage><epage>124</epage><pages>119-124</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Several strains of human cytomegalovirus including recent isolates, were grown in epithelial cells derived from thyroid tissue. 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source | MEDLINE; Springer Journals |
subjects | Cervix Uteri - microbiology Culture Techniques Cytomegalovirus - growth & development Cytomegalovirus - isolation & purification Cytopathogenic Effect, Viral Female Humans Thyroid Gland - microbiology Urine - microbiology Virus Cultivation - methods Virus Replication |
title | In-vitro cultivation of human cytomegalovirus in thyroid epithelial cells |
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