MICROBIAL PRODUCTION OF AMPHOTERICIN B-3H AND THE SYNTHESIS OF ITS SODIUM DESOXYCHOLATE (CARBOXYL-14C) COMPLEX AND METHYL-14C-ESTER
Amphotericin B-3H with a specific radioactivity in excess of 4 μCi/mg was produced by fermentation of Streptomyces nodosus IMRU 3694 in a complex medium, using acetate-3H as a precursor. The medium employed gave a production yield of 2.5-3.5 mg/ml of amphotericin B. The most efficient incorporation...
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| Veröffentlicht in: | Journal of antibiotics 1976, Vol.29(4), pp.438-443 |
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| container_end_page | 443 |
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| container_issue | 4 |
| container_start_page | 438 |
| container_title | Journal of antibiotics |
| container_volume | 29 |
| creator | MONJI, NOBUO MECHLINSKI, WITOLD SCHAFFNER, CARL P. |
| description | Amphotericin B-3H with a specific radioactivity in excess of 4 μCi/mg was produced by fermentation of Streptomyces nodosus IMRU 3694 in a complex medium, using acetate-3H as a precursor. The medium employed gave a production yield of 2.5-3.5 mg/ml of amphotericin B. The most efficient incorporation of acetate-3H occurred when the precursor was added at 24 hours after inoculation. It was observed, that the amount of amphotericin A co-produced in the fermentation broth with amphotericin B was significantly reduced by the addition of ethanol to the production medium. Complete inhibition of amphotericin A production was achieved at a level of 2% ethanol in the fermentation medium without an appreciable effect on the yield of amphotericin B. Methanol, 1-propanol, 2-propanol and 1-butanol lowered the yield of both antibiotics indiscriminately. The syntheses of amphotericin B-3H-Na desoxycholate (carboxyl-14C) complex and amphotericin B-3H methyl 14C-ester are also described. |
| doi_str_mv | 10.7164/antibiotics.29.438 |
| format | Article |
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The medium employed gave a production yield of 2.5-3.5 mg/ml of amphotericin B. The most efficient incorporation of acetate-3H occurred when the precursor was added at 24 hours after inoculation. It was observed, that the amount of amphotericin A co-produced in the fermentation broth with amphotericin B was significantly reduced by the addition of ethanol to the production medium. Complete inhibition of amphotericin A production was achieved at a level of 2% ethanol in the fermentation medium without an appreciable effect on the yield of amphotericin B. Methanol, 1-propanol, 2-propanol and 1-butanol lowered the yield of both antibiotics indiscriminately. The syntheses of amphotericin B-3H-Na desoxycholate (carboxyl-14C) complex and amphotericin B-3H methyl 14C-ester are also described.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.7164/antibiotics.29.438</identifier><identifier>PMID: 931813</identifier><language>eng</language><publisher>Japan: JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</publisher><subject>Acetates - metabolism ; Alcohols - pharmacology ; Amphotericin B - analogs & derivatives ; Amphotericin B - isolation & purification ; Amphotericin B - metabolism ; Deoxycholic Acid - chemical synthesis ; Depression, Chemical ; Esters - chemical synthesis ; Fermentation ; Streptomyces - drug effects ; Streptomyces - metabolism</subject><ispartof>The Journal of Antibiotics, 1976, Vol.29(4), pp.438-443</ispartof><rights>Japan Antibiotics Research Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4488-b89bd5ad21c3b8e8dca93e3e821b176ed6e16920e2eb6e25900f59ec50fd487c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/931813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MONJI, NOBUO</creatorcontrib><creatorcontrib>MECHLINSKI, WITOLD</creatorcontrib><creatorcontrib>SCHAFFNER, CARL P.</creatorcontrib><title>MICROBIAL PRODUCTION OF AMPHOTERICIN B-3H AND THE SYNTHESIS OF ITS SODIUM DESOXYCHOLATE (CARBOXYL-14C) COMPLEX AND METHYL-14C-ESTER</title><title>Journal of antibiotics</title><addtitle>J. Antibiot.</addtitle><description>Amphotericin B-3H with a specific radioactivity in excess of 4 μCi/mg was produced by fermentation of Streptomyces nodosus IMRU 3694 in a complex medium, using acetate-3H as a precursor. The medium employed gave a production yield of 2.5-3.5 mg/ml of amphotericin B. The most efficient incorporation of acetate-3H occurred when the precursor was added at 24 hours after inoculation. It was observed, that the amount of amphotericin A co-produced in the fermentation broth with amphotericin B was significantly reduced by the addition of ethanol to the production medium. Complete inhibition of amphotericin A production was achieved at a level of 2% ethanol in the fermentation medium without an appreciable effect on the yield of amphotericin B. Methanol, 1-propanol, 2-propanol and 1-butanol lowered the yield of both antibiotics indiscriminately. The syntheses of amphotericin B-3H-Na desoxycholate (carboxyl-14C) complex and amphotericin B-3H methyl 14C-ester are also described.</description><subject>Acetates - metabolism</subject><subject>Alcohols - pharmacology</subject><subject>Amphotericin B - analogs & derivatives</subject><subject>Amphotericin B - isolation & purification</subject><subject>Amphotericin B - metabolism</subject><subject>Deoxycholic Acid - chemical synthesis</subject><subject>Depression, Chemical</subject><subject>Esters - chemical synthesis</subject><subject>Fermentation</subject><subject>Streptomyces - drug effects</subject><subject>Streptomyces - metabolism</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMtu2zAQRYmiaeum_YGiC66CdiGXDz3IpUwrFRHJNCwZSFYERdGtAj9S0V503R-vYgVGNjMY3jtniAvAF4ymCY7DH2Z_7JrucOysnxI-DSl7AyaYMRzgMOZvwQQhggPGCPoAPnr_iBBNaMLeg3ecYobpBPwrpVipmUwLuFyp-VrUUi2guoVpucxVna2kkAs4C2gO08Uc1nkGq4fF0CpZPdtkXcFKzeW6hPOsUvcPIldFWmfwm0hXs2Euhq-I71Cocllk92dImdX5-B5k1XDiE7jamK13n1_6NVjfZrXIg0L9lCItAhuGjAUN400bmZZgSxvmWGsNp446RnCDk9i1scMxJ8gR18SORByhTcSdjdCmDVli6TW4GblP_eHPyfmj3nXeuu3W7N3h5DWjNIwYZYORjEbbH7zv3UY_9d3O9H81Rvo5eP0qeE24Ds9LX1_op2bn2svKmPQg343yoz-aX-4im36AbN1rIuYxO1PHMsAvLvvb9Nrt6X9K_pKE</recordid><startdate>1976</startdate><enddate>1976</enddate><creator>MONJI, NOBUO</creator><creator>MECHLINSKI, WITOLD</creator><creator>SCHAFFNER, CARL P.</creator><general>JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1976</creationdate><title>MICROBIAL PRODUCTION OF AMPHOTERICIN B-3H AND THE SYNTHESIS OF ITS SODIUM DESOXYCHOLATE (CARBOXYL-14C) COMPLEX AND METHYL-14C-ESTER</title><author>MONJI, NOBUO ; MECHLINSKI, WITOLD ; SCHAFFNER, CARL P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4488-b89bd5ad21c3b8e8dca93e3e821b176ed6e16920e2eb6e25900f59ec50fd487c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Acetates - metabolism</topic><topic>Alcohols - pharmacology</topic><topic>Amphotericin B - analogs & derivatives</topic><topic>Amphotericin B - isolation & purification</topic><topic>Amphotericin B - metabolism</topic><topic>Deoxycholic Acid - chemical synthesis</topic><topic>Depression, Chemical</topic><topic>Esters - chemical synthesis</topic><topic>Fermentation</topic><topic>Streptomyces - drug effects</topic><topic>Streptomyces - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MONJI, NOBUO</creatorcontrib><creatorcontrib>MECHLINSKI, WITOLD</creatorcontrib><creatorcontrib>SCHAFFNER, CARL P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antibiotics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MONJI, NOBUO</au><au>MECHLINSKI, WITOLD</au><au>SCHAFFNER, CARL P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MICROBIAL PRODUCTION OF AMPHOTERICIN B-3H AND THE SYNTHESIS OF ITS SODIUM DESOXYCHOLATE (CARBOXYL-14C) COMPLEX AND METHYL-14C-ESTER</atitle><jtitle>Journal of antibiotics</jtitle><addtitle>J. Antibiot.</addtitle><date>1976</date><risdate>1976</risdate><volume>29</volume><issue>4</issue><spage>438</spage><epage>443</epage><pages>438-443</pages><issn>0021-8820</issn><eissn>1881-1469</eissn><abstract>Amphotericin B-3H with a specific radioactivity in excess of 4 μCi/mg was produced by fermentation of Streptomyces nodosus IMRU 3694 in a complex medium, using acetate-3H as a precursor. The medium employed gave a production yield of 2.5-3.5 mg/ml of amphotericin B. The most efficient incorporation of acetate-3H occurred when the precursor was added at 24 hours after inoculation. It was observed, that the amount of amphotericin A co-produced in the fermentation broth with amphotericin B was significantly reduced by the addition of ethanol to the production medium. Complete inhibition of amphotericin A production was achieved at a level of 2% ethanol in the fermentation medium without an appreciable effect on the yield of amphotericin B. Methanol, 1-propanol, 2-propanol and 1-butanol lowered the yield of both antibiotics indiscriminately. The syntheses of amphotericin B-3H-Na desoxycholate (carboxyl-14C) complex and amphotericin B-3H methyl 14C-ester are also described.</abstract><cop>Japan</cop><pub>JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</pub><pmid>931813</pmid><doi>10.7164/antibiotics.29.438</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8820 |
| ispartof | The Journal of Antibiotics, 1976, Vol.29(4), pp.438-443 |
| issn | 0021-8820 1881-1469 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83345838 |
| source | J-STAGE Free; MEDLINE |
| subjects | Acetates - metabolism Alcohols - pharmacology Amphotericin B - analogs & derivatives Amphotericin B - isolation & purification Amphotericin B - metabolism Deoxycholic Acid - chemical synthesis Depression, Chemical Esters - chemical synthesis Fermentation Streptomyces - drug effects Streptomyces - metabolism |
| title | MICROBIAL PRODUCTION OF AMPHOTERICIN B-3H AND THE SYNTHESIS OF ITS SODIUM DESOXYCHOLATE (CARBOXYL-14C) COMPLEX AND METHYL-14C-ESTER |
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