Yeast alpha-isopropylmalate isomerase. Factors affecting stability and enzyme activity

Yeast alpha-isopropylmalate isomerase was found to be markedly stabilized by high concentrations of glycerol and (NH4)2SO4. Such conditions of high ionic strength inhibited the enzyme, stabilized the enzyme to heat, and affected kinetic parameters. The isomerase was found to exhibit ionic strength-d...

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Veröffentlicht in:	The Journal of biological chemistry 1976-06, Vol.251 (12), p.3545-3552
Hauptverfasser:	Bigelis, R, Umbarger, H E
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Sulfate - pharmacology Chelating Agents - pharmacology Chlorides - pharmacology Drug Stability Enzyme Activation - drug effects Fluorides - pharmacology Glycerol - pharmacology Hot Temperature Hydro-Lyases - metabolism Hydrogen-Ion Concentration Kinetics Phosphates - pharmacology Potassium - pharmacology Saccharomyces cerevisiae - enzymology Temperature
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