Effects of LH-RH on Isolated Pituitary Gonadotropic Cells
Summary Rat anterior pituitary glands were dissociated with Pronase and the cells were separated by velocity sedimentation at unit gravity. After 30 min of incubation of the enriched gonadotropic cells with LH-RH, there was a significant increase in LH and FSH in the incubation medium. LH-RH (100 ng...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1976-01, Vol.151 (1), p.160-162 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 151 |
| creator | Lloyd, Ricardo V. McShan, W. H |
| description | Summary
Rat anterior pituitary glands were dissociated with Pronase and the cells were separated by velocity sedimentation at unit gravity. After 30 min of incubation of the enriched gonadotropic cells with LH-RH, there was a significant increase in LH and FSH in the incubation medium. LH-RH (100 ng/ml) and 10−3
M cAMP both caused significant increases in LH in the incubation medium after 24 hr of incubation.
This investigation was supported by Public Health Service Training Grant IT05-GM 01932-03, The Ford Foundation Grant 630-0505A, and the National Institutes of Health Training Grant 5-T01-HD-00104-08.
The authors thank Dr. W. F. White of Abbott Laboratories for the synthetic LH-RH, and NIAMD, NIH for the rat FSH and LH radioimmunoassay kits. |
| doi_str_mv | 10.3181/00379727-151-39165 |
| format | Article |
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Rat anterior pituitary glands were dissociated with Pronase and the cells were separated by velocity sedimentation at unit gravity. After 30 min of incubation of the enriched gonadotropic cells with LH-RH, there was a significant increase in LH and FSH in the incubation medium. LH-RH (100 ng/ml) and 10−3
M cAMP both caused significant increases in LH in the incubation medium after 24 hr of incubation.
This investigation was supported by Public Health Service Training Grant IT05-GM 01932-03, The Ford Foundation Grant 630-0505A, and the National Institutes of Health Training Grant 5-T01-HD-00104-08.
The authors thank Dr. W. F. White of Abbott Laboratories for the synthetic LH-RH, and NIAMD, NIH for the rat FSH and LH radioimmunoassay kits.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-151-39165</identifier><identifier>PMID: 175370</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Bucladesine - pharmacology ; Cell Separation ; Follicle Stimulating Hormone - metabolism ; Gonadotropin-Releasing Hormone - pharmacology ; In Vitro Techniques ; Luteinizing Hormone - metabolism ; Male ; Pituitary Gland - drug effects ; Pituitary Gland, Anterior - cytology ; Pituitary Gland, Anterior - drug effects ; Pituitary Gland, Anterior - metabolism ; Rats ; Time Factors</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1976-01, Vol.151 (1), p.160-162</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/175370$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lloyd, Ricardo V.</creatorcontrib><creatorcontrib>McShan, W. H</creatorcontrib><title>Effects of LH-RH on Isolated Pituitary Gonadotropic Cells</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Rat anterior pituitary glands were dissociated with Pronase and the cells were separated by velocity sedimentation at unit gravity. After 30 min of incubation of the enriched gonadotropic cells with LH-RH, there was a significant increase in LH and FSH in the incubation medium. LH-RH (100 ng/ml) and 10−3
M cAMP both caused significant increases in LH in the incubation medium after 24 hr of incubation.
This investigation was supported by Public Health Service Training Grant IT05-GM 01932-03, The Ford Foundation Grant 630-0505A, and the National Institutes of Health Training Grant 5-T01-HD-00104-08.
The authors thank Dr. W. F. White of Abbott Laboratories for the synthetic LH-RH, and NIAMD, NIH for the rat FSH and LH radioimmunoassay kits.</description><subject>Animals</subject><subject>Bucladesine - pharmacology</subject><subject>Cell Separation</subject><subject>Follicle Stimulating Hormone - metabolism</subject><subject>Gonadotropin-Releasing Hormone - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Luteinizing Hormone - metabolism</subject><subject>Male</subject><subject>Pituitary Gland - drug effects</subject><subject>Pituitary Gland, Anterior - cytology</subject><subject>Pituitary Gland, Anterior - drug effects</subject><subject>Pituitary Gland, Anterior - metabolism</subject><subject>Rats</subject><subject>Time Factors</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOwzAUhi3ErRReADF4YjP1sevbiKrSIlUCIZgt17FRqjQucTLw9iRtERvTGf6L_vMhdAv0gYOGCaVcGcUUAQGEG5DiBI1AcEG4NOYUjQYDGRyX6CrnDaUgFJMX6ByU4IqOkJnHGHybcYp4tSRvS5xq_JxT5dpQ4Ney7crWNd94kWpXpLZJu9LjWaiqfI3OoqtyuDneMfp4mr_PlmT1sniePa6IZ1q3REal5NpwAVJ6D1OtjWBGw1RRZoSKIKGQlDpqQMtpPw68plwDYwWVPCo-RveH3l2TvrqQW7sts-8XuDqkLlvNOVOaDUZ2MPom5dyEaHdNue3HW6B2wGV_cdkel93j6kN3x_ZuvQ3FX2TPp5cnBzm7z2A3qWvq_tf_Cn8A_7pulw</recordid><startdate>197601</startdate><enddate>197601</enddate><creator>Lloyd, Ricardo V.</creator><creator>McShan, W. H</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197601</creationdate><title>Effects of LH-RH on Isolated Pituitary Gonadotropic Cells</title><author>Lloyd, Ricardo V. ; McShan, W. H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-6f776b935166cc14889529814702957f161d600a0918647261c8038122d063f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Bucladesine - pharmacology</topic><topic>Cell Separation</topic><topic>Follicle Stimulating Hormone - metabolism</topic><topic>Gonadotropin-Releasing Hormone - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Luteinizing Hormone - metabolism</topic><topic>Male</topic><topic>Pituitary Gland - drug effects</topic><topic>Pituitary Gland, Anterior - cytology</topic><topic>Pituitary Gland, Anterior - drug effects</topic><topic>Pituitary Gland, Anterior - metabolism</topic><topic>Rats</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lloyd, Ricardo V.</creatorcontrib><creatorcontrib>McShan, W. H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lloyd, Ricardo V.</au><au>McShan, W. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of LH-RH on Isolated Pituitary Gonadotropic Cells</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1976-01</date><risdate>1976</risdate><volume>151</volume><issue>1</issue><spage>160</spage><epage>162</epage><pages>160-162</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Rat anterior pituitary glands were dissociated with Pronase and the cells were separated by velocity sedimentation at unit gravity. After 30 min of incubation of the enriched gonadotropic cells with LH-RH, there was a significant increase in LH and FSH in the incubation medium. LH-RH (100 ng/ml) and 10−3
M cAMP both caused significant increases in LH in the incubation medium after 24 hr of incubation.
This investigation was supported by Public Health Service Training Grant IT05-GM 01932-03, The Ford Foundation Grant 630-0505A, and the National Institutes of Health Training Grant 5-T01-HD-00104-08.
The authors thank Dr. W. F. White of Abbott Laboratories for the synthetic LH-RH, and NIAMD, NIH for the rat FSH and LH radioimmunoassay kits.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>175370</pmid><doi>10.3181/00379727-151-39165</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1976-01, Vol.151 (1), p.160-162 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Bucladesine - pharmacology Cell Separation Follicle Stimulating Hormone - metabolism Gonadotropin-Releasing Hormone - pharmacology In Vitro Techniques Luteinizing Hormone - metabolism Male Pituitary Gland - drug effects Pituitary Gland, Anterior - cytology Pituitary Gland, Anterior - drug effects Pituitary Gland, Anterior - metabolism Rats Time Factors |
| title | Effects of LH-RH on Isolated Pituitary Gonadotropic Cells |
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