Stoichiometry of polypeptide chain elongation

To quantitate the amount of GTP hydrolyzed during polypeptide chain elongation, an in vitro system containing purified endogenous Escherichia coli polysomes has been developed. The polysomes are washed with 1 M NH4Cl to eliminate endogenous GTPase activities and are depleted of subunits and free rib...

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Veröffentlicht in:	The Journal of biological chemistry 1976-03, Vol.251 (6), p.1718-1722
Hauptverfasser:	Cabrer, B, San-Millian, M J, Vazquez, D, Modolell, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Escherichia coli - metabolism Guanosine Triphosphate - metabolism Kinetics Peptide Chain Elongation, Translational Peptide Elongation Factors Polyribosomes - metabolism Protein Binding Transfer RNA Aminoacylation
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