Gradient, polyacrylamide gel electrophoresis of proteins from cytotoxic mycoplasma membranes
Membranes of Mycoplasma pneumoniae were prepared following several different cell lysis procedures (freeze-thaw, french press, digitonin, sonication, osmotic shock, pH shock). All possessed some degree of toxicity for ciliated epithelial cells, but the french press and freeze-thaw processes were opt...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1976-05, Vol.70 (1), p.271-279 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Gabridge, M.G. Singer, S.E. Esposito, R.A. |
| description | Membranes of
Mycoplasma
pneumoniae
were prepared following several different cell lysis procedures (freeze-thaw, french press, digitonin, sonication, osmotic shock, pH shock). All possessed some degree of toxicity for ciliated epithelial cells, but the french press and freeze-thaw processes were optimal in terms of lysis efficiency, membrane yield, and cytotoxicity. Membrane proteins from virulent and attenuated mycoplasmas were electrophoresed with a new, high resolution electrophoresis technique (gradient polyacrylamide gels), and strain differences were detectable among the 50+ discernible bands. |
| doi_str_mv | 10.1016/0006-291X(76)91138-4 |
| format | Article |
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Mycoplasma
pneumoniae
were prepared following several different cell lysis procedures (freeze-thaw, french press, digitonin, sonication, osmotic shock, pH shock). All possessed some degree of toxicity for ciliated epithelial cells, but the french press and freeze-thaw processes were optimal in terms of lysis efficiency, membrane yield, and cytotoxicity. Membrane proteins from virulent and attenuated mycoplasmas were electrophoresed with a new, high resolution electrophoresis technique (gradient polyacrylamide gels), and strain differences were detectable among the 50+ discernible bands.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(76)91138-4</identifier><identifier>PMID: 1275941</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacterial Proteins - analysis ; Biological Assay ; Cell Membrane - analysis ; Electrophoresis, Polyacrylamide Gel ; Mycoplasma - analysis ; Species Specificity ; Toxins, Biological - analysis</subject><ispartof>Biochemical and biophysical research communications, 1976-05, Vol.70 (1), p.271-279</ispartof><rights>1976</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-b091330a5283a01d072e54873a4347d2e52b2997aaa3c0b9277bd351f434f883</citedby><cites>FETCH-LOGICAL-c357t-b091330a5283a01d072e54873a4347d2e52b2997aaa3c0b9277bd351f434f883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X76911384$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1275941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gabridge, M.G.</creatorcontrib><creatorcontrib>Singer, S.E.</creatorcontrib><creatorcontrib>Esposito, R.A.</creatorcontrib><title>Gradient, polyacrylamide gel electrophoresis of proteins from cytotoxic mycoplasma membranes</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Membranes of
Mycoplasma
pneumoniae
were prepared following several different cell lysis procedures (freeze-thaw, french press, digitonin, sonication, osmotic shock, pH shock). All possessed some degree of toxicity for ciliated epithelial cells, but the french press and freeze-thaw processes were optimal in terms of lysis efficiency, membrane yield, and cytotoxicity. Membrane proteins from virulent and attenuated mycoplasmas were electrophoresed with a new, high resolution electrophoresis technique (gradient polyacrylamide gels), and strain differences were detectable among the 50+ discernible bands.</description><subject>Bacterial Proteins - analysis</subject><subject>Biological Assay</subject><subject>Cell Membrane - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Mycoplasma - analysis</subject><subject>Species Specificity</subject><subject>Toxins, Biological - analysis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFrFTEQxoMo7Wv1P2ghJ2nB1ZlNdrO5FErRKhS89OBBCNnsbI1sXrbJPnH_e_N8xd48DcP3zcw3P8bOEN4jYPsBANqq1vjtQrWXGlF0lXzBNggaqhpBvmSbf5ZjdpLzTwBE2eojdoS1arTEDft-m-zgabu843OcVuvSOtngB-IPNHGayC0pzj9iouwzjyOfU1zIbzMfUwzcrUtc4m_veFhdnCebg-WBQp_slvJr9mq0U6Y3T_WU3X_6eH_zubr7evvl5vqucqJRS9WDRiHANnUnLOAAqqZGdkpYKaQaSlP3tdbKWisc9LpWqh9Eg2ORx64Tp-ztYW3J9rijvJjgs6NpKhniLptOCIQOVTHKg9GlmHOi0czJB5tWg2D2TM0emNkDM6o1f5kaWcbOn_bv-kDD89ABYtGvDjqVH395Sia7gtTR4FPhZ4bo_3_gDxGMhuY</recordid><startdate>19760503</startdate><enddate>19760503</enddate><creator>Gabridge, M.G.</creator><creator>Singer, S.E.</creator><creator>Esposito, R.A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19760503</creationdate><title>Gradient, polyacrylamide gel electrophoresis of proteins from cytotoxic mycoplasma membranes</title><author>Gabridge, M.G. ; Singer, S.E. ; Esposito, R.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-b091330a5283a01d072e54873a4347d2e52b2997aaa3c0b9277bd351f434f883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Bacterial Proteins - analysis</topic><topic>Biological Assay</topic><topic>Cell Membrane - analysis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Mycoplasma - analysis</topic><topic>Species Specificity</topic><topic>Toxins, Biological - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gabridge, M.G.</creatorcontrib><creatorcontrib>Singer, S.E.</creatorcontrib><creatorcontrib>Esposito, R.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gabridge, M.G.</au><au>Singer, S.E.</au><au>Esposito, R.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gradient, polyacrylamide gel electrophoresis of proteins from cytotoxic mycoplasma membranes</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1976-05-03</date><risdate>1976</risdate><volume>70</volume><issue>1</issue><spage>271</spage><epage>279</epage><pages>271-279</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Membranes of
Mycoplasma
pneumoniae
were prepared following several different cell lysis procedures (freeze-thaw, french press, digitonin, sonication, osmotic shock, pH shock). All possessed some degree of toxicity for ciliated epithelial cells, but the french press and freeze-thaw processes were optimal in terms of lysis efficiency, membrane yield, and cytotoxicity. Membrane proteins from virulent and attenuated mycoplasmas were electrophoresed with a new, high resolution electrophoresis technique (gradient polyacrylamide gels), and strain differences were detectable among the 50+ discernible bands.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>1275941</pmid><doi>10.1016/0006-291X(76)91138-4</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1976-05, Vol.70 (1), p.271-279 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83310817 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial Proteins - analysis Biological Assay Cell Membrane - analysis Electrophoresis, Polyacrylamide Gel Mycoplasma - analysis Species Specificity Toxins, Biological - analysis |
| title | Gradient, polyacrylamide gel electrophoresis of proteins from cytotoxic mycoplasma membranes |
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