Membrane (M) protein of HVJ (Sendai virus): Its role in virus assembly
The role in virus assembly of membrane or matrix (M) protein of HVJ (Sendai virus) was investigated. When virions were disrupted with alkali Tween 20, nucleocapsids with a sheathlike cover on the surface and a width of approx 33 nm were often obtained, and this sheathlike structure was composed of M...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1976-05, Vol.71 (1), p.143-161 |
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| container_title | Virology (New York, N.Y.) |
| container_volume | 71 |
| creator | Yoshida, Tetsuya Nagai, Yoshiyuki Yoshii, Saiji Maeno, Koichiro Matsumoto, Toshisada Hoshino, Munemitsu |
| description | The role in virus assembly of membrane or matrix (M) protein of HVJ (Sendai virus) was investigated. When virions were disrupted with alkali Tween 20, nucleocapsids with a sheathlike cover on the surface and a width of approx 33 nm were often obtained, and this sheathlike structure was composed of M protein. Selective aggregation of viral components
in vitro was examined. Each viral component was isolated separately from virions by using detergents, and they were mixed with each other in various combinations. The structures which reaggregated were examined for their protein composition, and the results indicate that the nucleocapsid protein does not combine with viral glycoproteins to form a coaggregate unless M protein is present, which suggests that the M protein
in vivo may be a mediator for the association of nucleocapsid with the modified areas of plasma membrane which will become viral envelope. |
| doi_str_mv | 10.1016/0042-6822(76)90101-X |
| format | Article |
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in vitro was examined. Each viral component was isolated separately from virions by using detergents, and they were mixed with each other in various combinations. The structures which reaggregated were examined for their protein composition, and the results indicate that the nucleocapsid protein does not combine with viral glycoproteins to form a coaggregate unless M protein is present, which suggests that the M protein
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in vitro was examined. Each viral component was isolated separately from virions by using detergents, and they were mixed with each other in various combinations. The structures which reaggregated were examined for their protein composition, and the results indicate that the nucleocapsid protein does not combine with viral glycoproteins to form a coaggregate unless M protein is present, which suggests that the M protein
in vivo may be a mediator for the association of nucleocapsid with the modified areas of plasma membrane which will become viral envelope.</description><subject>Antigens, Viral</subject><subject>Capsid - metabolism</subject><subject>Cell Line</subject><subject>Cell Membrane - metabolism</subject><subject>Glycoproteins - metabolism</subject><subject>Parainfluenza Virus 1, Human - growth & development</subject><subject>Parainfluenza Virus 1, Human - metabolism</subject><subject>Parainfluenza Virus 1, Human - ultrastructure</subject><subject>Sarcosine - pharmacology</subject><subject>Viral Proteins - analysis</subject><subject>Viral Proteins - immunology</subject><subject>Viral Proteins - metabolism</subject><subject>Virus Replication</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kElLAzEUx4O41eo36CEnaQ-jySzJxIMgxVqlxYMLvYVM8gYiM52azBT67U0X9OYp5P0X3vshNKDkhhLKbglJ44jlcTzkbCRImEWLI9SjRLCIJCk9Rr1fyzm68P6LhD_n5AydUi6oED00mUNdOLUEPJyP8Mo1Ldglbko8_XzBwzdYGmXx2rrOj-7wc-uxayrAwbKbYeV9yFebS3RSqsrD1eHto4_J4_t4Gs1en57HD7NIp3HcRgJAmyxLeMYKyoimTOnckIRksTZlQkptKAURFMULRkDnmgZdiYybLKU06aPrfW_Y9LsD38raeg1VFS5oOi_zJCExY1kwpnujdo33Dkq5crZWbiMpkVt6cotGbtFIzuSOnlyE2ODQ3xU1mL_QDleQ7_cyhBvXFpz02sJSg7EOdCtNY__v_wHuQnxO</recordid><startdate>197605</startdate><enddate>197605</enddate><creator>Yoshida, Tetsuya</creator><creator>Nagai, Yoshiyuki</creator><creator>Yoshii, Saiji</creator><creator>Maeno, Koichiro</creator><creator>Matsumoto, Toshisada</creator><creator>Hoshino, Munemitsu</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197605</creationdate><title>Membrane (M) protein of HVJ (Sendai virus): Its role in virus assembly</title><author>Yoshida, Tetsuya ; Nagai, Yoshiyuki ; Yoshii, Saiji ; Maeno, Koichiro ; Matsumoto, Toshisada ; Hoshino, Munemitsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-9eecd553756b160c16ac8d03052cdf30fcd11e960ca7b60ec8c18d0a957d54113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Antigens, Viral</topic><topic>Capsid - metabolism</topic><topic>Cell Line</topic><topic>Cell Membrane - metabolism</topic><topic>Glycoproteins - metabolism</topic><topic>Parainfluenza Virus 1, Human - growth & development</topic><topic>Parainfluenza Virus 1, Human - metabolism</topic><topic>Parainfluenza Virus 1, Human - ultrastructure</topic><topic>Sarcosine - pharmacology</topic><topic>Viral Proteins - analysis</topic><topic>Viral Proteins - immunology</topic><topic>Viral Proteins - metabolism</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshida, Tetsuya</creatorcontrib><creatorcontrib>Nagai, Yoshiyuki</creatorcontrib><creatorcontrib>Yoshii, Saiji</creatorcontrib><creatorcontrib>Maeno, Koichiro</creatorcontrib><creatorcontrib>Matsumoto, Toshisada</creatorcontrib><creatorcontrib>Hoshino, Munemitsu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshida, Tetsuya</au><au>Nagai, Yoshiyuki</au><au>Yoshii, Saiji</au><au>Maeno, Koichiro</au><au>Matsumoto, Toshisada</au><au>Hoshino, Munemitsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Membrane (M) protein of HVJ (Sendai virus): Its role in virus assembly</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1976-05</date><risdate>1976</risdate><volume>71</volume><issue>1</issue><spage>143</spage><epage>161</epage><pages>143-161</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The role in virus assembly of membrane or matrix (M) protein of HVJ (Sendai virus) was investigated. When virions were disrupted with alkali Tween 20, nucleocapsids with a sheathlike cover on the surface and a width of approx 33 nm were often obtained, and this sheathlike structure was composed of M protein. Selective aggregation of viral components
in vitro was examined. Each viral component was isolated separately from virions by using detergents, and they were mixed with each other in various combinations. The structures which reaggregated were examined for their protein composition, and the results indicate that the nucleocapsid protein does not combine with viral glycoproteins to form a coaggregate unless M protein is present, which suggests that the M protein
in vivo may be a mediator for the association of nucleocapsid with the modified areas of plasma membrane which will become viral envelope.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>179199</pmid><doi>10.1016/0042-6822(76)90101-X</doi><tpages>19</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1976-05, Vol.71 (1), p.143-161 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Antigens, Viral Capsid - metabolism Cell Line Cell Membrane - metabolism Glycoproteins - metabolism Parainfluenza Virus 1, Human - growth & development Parainfluenza Virus 1, Human - metabolism Parainfluenza Virus 1, Human - ultrastructure Sarcosine - pharmacology Viral Proteins - analysis Viral Proteins - immunology Viral Proteins - metabolism Virus Replication |
| title | Membrane (M) protein of HVJ (Sendai virus): Its role in virus assembly |
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