Fine Structure of a Murine Mammary Carcinoma Cell Line
A fine structural study was made of cells from the epithelioid MCF-8/5-2A cell line derived from a MuMTV-free, D2 transplantable hyperplastic outgrowth. Electron microscopy shows the cells to be truly epithelial with many cell-to-cell junctions and microvilli. The cells are similar in many respects...
Gespeichert in:
| Veröffentlicht in: | In Vitro 1976-01, Vol.12 (1), p.57-64 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 64 |
|---|---|
| container_issue | 1 |
| container_start_page | 57 |
| container_title | In Vitro |
| container_volume | 12 |
| creator | Arnold, Wm. James Soule, Herbert D. Russo, Jose |
| description | A fine structural study was made of cells from the epithelioid MCF-8/5-2A cell line derived from a MuMTV-free, D2 transplantable hyperplastic outgrowth. Electron microscopy shows the cells to be truly epithelial with many cell-to-cell junctions and microvilli. The cells are similar in many respects to normal mouse mammary gland and some of the conventional mammary tumor derived cell lines. This study supports previous observations of the absence of MuMTV in MCF-8 within the limits of morphological detection, and demonstrates the presence of numerous virus particles within, or budding into, cisternae of the endoplasmic reticulum and nuclear envelope. These intracisternal A particles have not been previously described in such abundance in mammary tumor tissue culture cells. |
| doi_str_mv | 10.1007/BF02832794 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_83259406</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>20170264</jstor_id><sourcerecordid>20170264</sourcerecordid><originalsourceid>FETCH-LOGICAL-c303t-9db63a35682d7d9b11a74483a97de76a5bbf251f65fea9f0fdc40399bc7ef3a53</originalsourceid><addsrcrecordid>eNpFkD1PwzAQhi3EVykszCB5YkAKnL_jsUQUkFIxAHPkOLaUqmmKnQz8e1xa0emkex69unsRuibwQADU49McaM6o0vwITQhXIqMy18dokiDLhBTiHF3EuARgICk5Q6dEUc70BMl5u3b4YwijHcbgcO-xwYsxbLcL03Um_ODCBNuu-87gwq1WuEzsEp14s4ruaj-n6Gv-_Fm8ZuX7y1sxKzPLgA2ZbmrJDBMyp41qdE2IUZznzGjVOCWNqGtPBfFSeGe0B99YDkzr2irnmRFsiu52uZvQf48uDlXXRpuuMGvXj7FKTwvNQSbxfifa0McYnK82od1eXxGoth1Vh46SfLNPHevONQf1r5SEb3d4GYc-_FMKRAGVnP0Cmz9o6A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83259406</pqid></control><display><type>article</type><title>Fine Structure of a Murine Mammary Carcinoma Cell Line</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>SpringerLink Journals - AutoHoldings</source><creator>Arnold, Wm. James ; Soule, Herbert D. ; Russo, Jose</creator><creatorcontrib>Arnold, Wm. James ; Soule, Herbert D. ; Russo, Jose</creatorcontrib><description>A fine structural study was made of cells from the epithelioid MCF-8/5-2A cell line derived from a MuMTV-free, D2 transplantable hyperplastic outgrowth. Electron microscopy shows the cells to be truly epithelial with many cell-to-cell junctions and microvilli. The cells are similar in many respects to normal mouse mammary gland and some of the conventional mammary tumor derived cell lines. This study supports previous observations of the absence of MuMTV in MCF-8 within the limits of morphological detection, and demonstrates the presence of numerous virus particles within, or budding into, cisternae of the endoplasmic reticulum and nuclear envelope. These intracisternal A particles have not been previously described in such abundance in mammary tumor tissue culture cells.</description><identifier>ISSN: 0073-5655</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/BF02832794</identifier><identifier>PMID: 172439</identifier><language>eng</language><publisher>United States: Tissue Culture Association, Inc</publisher><subject>Animals ; Budding ; Cell Adhesion ; Cell Line ; Cell lines ; Cell membranes ; Cell Nucleolus - ultrastructure ; Cell Nucleus - ultrastructure ; Chromatin - ultrastructure ; Dendritic cells ; Desmosomes - ultrastructure ; Endoplasmic Reticulum - ultrastructure ; Epithelial cells ; Golgi Apparatus - ultrastructure ; Inclusion Bodies - ultrastructure ; Lysosomes - ultrastructure ; Mammary Neoplasms, Experimental - pathology ; Mammary Tumor Virus, Mouse - ultrastructure ; Mice ; Microtubules - ultrastructure ; Mitochondria - ultrastructure ; Nuclear membrane ; Tumor cell line ; Tumors ; Virions ; Viruses</subject><ispartof>In Vitro, 1976-01, Vol.12 (1), p.57-64</ispartof><rights>Copyright 1976 Tissue Culture Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-9db63a35682d7d9b11a74483a97de76a5bbf251f65fea9f0fdc40399bc7ef3a53</citedby><cites>FETCH-LOGICAL-c303t-9db63a35682d7d9b11a74483a97de76a5bbf251f65fea9f0fdc40399bc7ef3a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20170264$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20170264$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/172439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arnold, Wm. James</creatorcontrib><creatorcontrib>Soule, Herbert D.</creatorcontrib><creatorcontrib>Russo, Jose</creatorcontrib><title>Fine Structure of a Murine Mammary Carcinoma Cell Line</title><title>In Vitro</title><addtitle>In Vitro</addtitle><description>A fine structural study was made of cells from the epithelioid MCF-8/5-2A cell line derived from a MuMTV-free, D2 transplantable hyperplastic outgrowth. Electron microscopy shows the cells to be truly epithelial with many cell-to-cell junctions and microvilli. The cells are similar in many respects to normal mouse mammary gland and some of the conventional mammary tumor derived cell lines. This study supports previous observations of the absence of MuMTV in MCF-8 within the limits of morphological detection, and demonstrates the presence of numerous virus particles within, or budding into, cisternae of the endoplasmic reticulum and nuclear envelope. These intracisternal A particles have not been previously described in such abundance in mammary tumor tissue culture cells.</description><subject>Animals</subject><subject>Budding</subject><subject>Cell Adhesion</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell membranes</subject><subject>Cell Nucleolus - ultrastructure</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Chromatin - ultrastructure</subject><subject>Dendritic cells</subject><subject>Desmosomes - ultrastructure</subject><subject>Endoplasmic Reticulum - ultrastructure</subject><subject>Epithelial cells</subject><subject>Golgi Apparatus - ultrastructure</subject><subject>Inclusion Bodies - ultrastructure</subject><subject>Lysosomes - ultrastructure</subject><subject>Mammary Neoplasms, Experimental - pathology</subject><subject>Mammary Tumor Virus, Mouse - ultrastructure</subject><subject>Mice</subject><subject>Microtubules - ultrastructure</subject><subject>Mitochondria - ultrastructure</subject><subject>Nuclear membrane</subject><subject>Tumor cell line</subject><subject>Tumors</subject><subject>Virions</subject><subject>Viruses</subject><issn>0073-5655</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAQhi3EVykszCB5YkAKnL_jsUQUkFIxAHPkOLaUqmmKnQz8e1xa0emkex69unsRuibwQADU49McaM6o0vwITQhXIqMy18dokiDLhBTiHF3EuARgICk5Q6dEUc70BMl5u3b4YwijHcbgcO-xwYsxbLcL03Um_ODCBNuu-87gwq1WuEzsEp14s4ruaj-n6Gv-_Fm8ZuX7y1sxKzPLgA2ZbmrJDBMyp41qdE2IUZznzGjVOCWNqGtPBfFSeGe0B99YDkzr2irnmRFsiu52uZvQf48uDlXXRpuuMGvXj7FKTwvNQSbxfifa0McYnK82od1eXxGoth1Vh46SfLNPHevONQf1r5SEb3d4GYc-_FMKRAGVnP0Cmz9o6A</recordid><startdate>19760101</startdate><enddate>19760101</enddate><creator>Arnold, Wm. James</creator><creator>Soule, Herbert D.</creator><creator>Russo, Jose</creator><general>Tissue Culture Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19760101</creationdate><title>Fine Structure of a Murine Mammary Carcinoma Cell Line</title><author>Arnold, Wm. James ; Soule, Herbert D. ; Russo, Jose</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-9db63a35682d7d9b11a74483a97de76a5bbf251f65fea9f0fdc40399bc7ef3a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Budding</topic><topic>Cell Adhesion</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell membranes</topic><topic>Cell Nucleolus - ultrastructure</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Chromatin - ultrastructure</topic><topic>Dendritic cells</topic><topic>Desmosomes - ultrastructure</topic><topic>Endoplasmic Reticulum - ultrastructure</topic><topic>Epithelial cells</topic><topic>Golgi Apparatus - ultrastructure</topic><topic>Inclusion Bodies - ultrastructure</topic><topic>Lysosomes - ultrastructure</topic><topic>Mammary Neoplasms, Experimental - pathology</topic><topic>Mammary Tumor Virus, Mouse - ultrastructure</topic><topic>Mice</topic><topic>Microtubules - ultrastructure</topic><topic>Mitochondria - ultrastructure</topic><topic>Nuclear membrane</topic><topic>Tumor cell line</topic><topic>Tumors</topic><topic>Virions</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arnold, Wm. James</creatorcontrib><creatorcontrib>Soule, Herbert D.</creatorcontrib><creatorcontrib>Russo, Jose</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arnold, Wm. James</au><au>Soule, Herbert D.</au><au>Russo, Jose</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fine Structure of a Murine Mammary Carcinoma Cell Line</atitle><jtitle>In Vitro</jtitle><addtitle>In Vitro</addtitle><date>1976-01-01</date><risdate>1976</risdate><volume>12</volume><issue>1</issue><spage>57</spage><epage>64</epage><pages>57-64</pages><issn>0073-5655</issn><eissn>1475-2689</eissn><abstract>A fine structural study was made of cells from the epithelioid MCF-8/5-2A cell line derived from a MuMTV-free, D2 transplantable hyperplastic outgrowth. Electron microscopy shows the cells to be truly epithelial with many cell-to-cell junctions and microvilli. The cells are similar in many respects to normal mouse mammary gland and some of the conventional mammary tumor derived cell lines. This study supports previous observations of the absence of MuMTV in MCF-8 within the limits of morphological detection, and demonstrates the presence of numerous virus particles within, or budding into, cisternae of the endoplasmic reticulum and nuclear envelope. These intracisternal A particles have not been previously described in such abundance in mammary tumor tissue culture cells.</abstract><cop>United States</cop><pub>Tissue Culture Association, Inc</pub><pmid>172439</pmid><doi>10.1007/BF02832794</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0073-5655 |
| ispartof | In Vitro, 1976-01, Vol.12 (1), p.57-64 |
| issn | 0073-5655 1475-2689 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83259406 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; SpringerLink Journals - AutoHoldings |
| subjects | Animals Budding Cell Adhesion Cell Line Cell lines Cell membranes Cell Nucleolus - ultrastructure Cell Nucleus - ultrastructure Chromatin - ultrastructure Dendritic cells Desmosomes - ultrastructure Endoplasmic Reticulum - ultrastructure Epithelial cells Golgi Apparatus - ultrastructure Inclusion Bodies - ultrastructure Lysosomes - ultrastructure Mammary Neoplasms, Experimental - pathology Mammary Tumor Virus, Mouse - ultrastructure Mice Microtubules - ultrastructure Mitochondria - ultrastructure Nuclear membrane Tumor cell line Tumors Virions Viruses |
| title | Fine Structure of a Murine Mammary Carcinoma Cell Line |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T15%3A24%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fine%20Structure%20of%20a%20Murine%20Mammary%20Carcinoma%20Cell%20Line&rft.jtitle=In%20Vitro&rft.au=Arnold,%20Wm.%20James&rft.date=1976-01-01&rft.volume=12&rft.issue=1&rft.spage=57&rft.epage=64&rft.pages=57-64&rft.issn=0073-5655&rft.eissn=1475-2689&rft_id=info:doi/10.1007/BF02832794&rft_dat=%3Cjstor_proqu%3E20170264%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=83259406&rft_id=info:pmid/172439&rft_jstor_id=20170264&rfr_iscdi=true |