Induction of thymidylate synthetase activity in Tetrahymena by cyclic guanosine monophosphate
The induction of TMP synthetase activity in Tetrahymena pyriformis depended upon growth conditions. Enzymatic activity was low in cells grown in complex medium, and was high in cells grown in, or shifted to, defined medium. TMP synthetase activity rose 5 hours after the shift from complex to defined...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1975-12, Vol.67 (4), p.1319-1325 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Dickens, Michael S. Lucas-Lenard, Jean Roth, Jay S. |
| description | The induction of TMP synthetase activity in
Tetrahymena pyriformis depended upon growth conditions. Enzymatic activity was low in cells grown in complex medium, and was high in cells grown in, or shifted to, defined medium. TMP synthetase activity rose 5 hours after the shift from complex to defined medium using uracil as the pyrimidine source. The time of induction was decreased to
3
1
2
hours using dUMP as the pyrimidine source. cGMP or its dibutyryl derivative, but not cAMP, caused the induction of TMP synthetase activity in cells grown in complex medium. Caffeine, but not theophylline, mimicked the cGMP response. cAMP decreased both the cGMP and caffeine mediated increases in TMP synthetase activity. This is the first demonstration of an effect of cGMP on induction of an enzyme of pyrimidine metabolism in any cellular system. |
| doi_str_mv | 10.1016/0006-291X(75)90171-0 |
| format | Article |
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Tetrahymena pyriformis depended upon growth conditions. Enzymatic activity was low in cells grown in complex medium, and was high in cells grown in, or shifted to, defined medium. TMP synthetase activity rose 5 hours after the shift from complex to defined medium using uracil as the pyrimidine source. The time of induction was decreased to
3
1
2
hours using dUMP as the pyrimidine source. cGMP or its dibutyryl derivative, but not cAMP, caused the induction of TMP synthetase activity in cells grown in complex medium. Caffeine, but not theophylline, mimicked the cGMP response. cAMP decreased both the cGMP and caffeine mediated increases in TMP synthetase activity. This is the first demonstration of an effect of cGMP on induction of an enzyme of pyrimidine metabolism in any cellular system.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(75)90171-0</identifier><identifier>PMID: 173339</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bucladesine - pharmacology ; Caffeine - pharmacology ; Cell Division ; Culture Media ; Cyclic GMP - analogs & derivatives ; Cyclic GMP - pharmacology ; Enzyme Induction - drug effects ; Methyltransferases - biosynthesis ; Tetrahymena pyriformis - drug effects ; Tetrahymena pyriformis - enzymology ; Theophylline - pharmacology ; Thymidylate Synthase - biosynthesis</subject><ispartof>Biochemical and biophysical research communications, 1975-12, Vol.67 (4), p.1319-1325</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-df29e4cc6d6c40e7d74005c4f37663d26a6756c8ec98050f0bd8c34f8c1c7bb3</citedby><cites>FETCH-LOGICAL-c356t-df29e4cc6d6c40e7d74005c4f37663d26a6756c8ec98050f0bd8c34f8c1c7bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(75)90171-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/173339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dickens, Michael S.</creatorcontrib><creatorcontrib>Lucas-Lenard, Jean</creatorcontrib><creatorcontrib>Roth, Jay S.</creatorcontrib><title>Induction of thymidylate synthetase activity in Tetrahymena by cyclic guanosine monophosphate</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The induction of TMP synthetase activity in
Tetrahymena pyriformis depended upon growth conditions. Enzymatic activity was low in cells grown in complex medium, and was high in cells grown in, or shifted to, defined medium. TMP synthetase activity rose 5 hours after the shift from complex to defined medium using uracil as the pyrimidine source. The time of induction was decreased to
3
1
2
hours using dUMP as the pyrimidine source. cGMP or its dibutyryl derivative, but not cAMP, caused the induction of TMP synthetase activity in cells grown in complex medium. Caffeine, but not theophylline, mimicked the cGMP response. cAMP decreased both the cGMP and caffeine mediated increases in TMP synthetase activity. This is the first demonstration of an effect of cGMP on induction of an enzyme of pyrimidine metabolism in any cellular system.</description><subject>Animals</subject><subject>Bucladesine - pharmacology</subject><subject>Caffeine - pharmacology</subject><subject>Cell Division</subject><subject>Culture Media</subject><subject>Cyclic GMP - analogs & derivatives</subject><subject>Cyclic GMP - pharmacology</subject><subject>Enzyme Induction - drug effects</subject><subject>Methyltransferases - biosynthesis</subject><subject>Tetrahymena pyriformis - drug effects</subject><subject>Tetrahymena pyriformis - enzymology</subject><subject>Theophylline - pharmacology</subject><subject>Thymidylate Synthase - biosynthesis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFv1DAQhS0EhaXlH_TgEyqHwHjt2MkFCVWlVKrEZQ9ckOWMJ6xRYi-xUyn_nmy3gltPc3jfe9J8jF0K-ChA6E8AoKttK35cmfpDC8KICl6wjYAWqq0A9ZJt_iFv2NucfwMIoXT7mp0JI6VsN-znXfQzlpAiTz0v-2UMfhlcIZ6XWPZUXCbuVuAhlIWHyHdUJrdiFB3vFo4LDgH5r9nFlEMkPqaYDvuUD_t15IK96t2Q6d3TPWe7rze762_V_ffbu-sv9xXKWpfK99uWFKL2GhWQ8UYB1Kh6abSWfqudNrXGhrBtoIYeOt-gVH2DAk3XyXP2_jR7mNKfmXKxY8hIw-AipTnbRopWKCNWUJ1AnFLOE_X2MIXRTYsVYI9O7VGYPQqzpraPTi2stcun_bkbyf8vPUpc48-nmNYXHwJNNmOgiOTDRFisT-H5_b8USogy</recordid><startdate>19751215</startdate><enddate>19751215</enddate><creator>Dickens, Michael S.</creator><creator>Lucas-Lenard, Jean</creator><creator>Roth, Jay S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19751215</creationdate><title>Induction of thymidylate synthetase activity in Tetrahymena by cyclic guanosine monophosphate</title><author>Dickens, Michael S. ; Lucas-Lenard, Jean ; Roth, Jay S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-df29e4cc6d6c40e7d74005c4f37663d26a6756c8ec98050f0bd8c34f8c1c7bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Bucladesine - pharmacology</topic><topic>Caffeine - pharmacology</topic><topic>Cell Division</topic><topic>Culture Media</topic><topic>Cyclic GMP - analogs & derivatives</topic><topic>Cyclic GMP - pharmacology</topic><topic>Enzyme Induction - drug effects</topic><topic>Methyltransferases - biosynthesis</topic><topic>Tetrahymena pyriformis - drug effects</topic><topic>Tetrahymena pyriformis - enzymology</topic><topic>Theophylline - pharmacology</topic><topic>Thymidylate Synthase - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dickens, Michael S.</creatorcontrib><creatorcontrib>Lucas-Lenard, Jean</creatorcontrib><creatorcontrib>Roth, Jay S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dickens, Michael S.</au><au>Lucas-Lenard, Jean</au><au>Roth, Jay S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of thymidylate synthetase activity in Tetrahymena by cyclic guanosine monophosphate</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1975-12-15</date><risdate>1975</risdate><volume>67</volume><issue>4</issue><spage>1319</spage><epage>1325</epage><pages>1319-1325</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The induction of TMP synthetase activity in
Tetrahymena pyriformis depended upon growth conditions. Enzymatic activity was low in cells grown in complex medium, and was high in cells grown in, or shifted to, defined medium. TMP synthetase activity rose 5 hours after the shift from complex to defined medium using uracil as the pyrimidine source. The time of induction was decreased to
3
1
2
hours using dUMP as the pyrimidine source. cGMP or its dibutyryl derivative, but not cAMP, caused the induction of TMP synthetase activity in cells grown in complex medium. Caffeine, but not theophylline, mimicked the cGMP response. cAMP decreased both the cGMP and caffeine mediated increases in TMP synthetase activity. This is the first demonstration of an effect of cGMP on induction of an enzyme of pyrimidine metabolism in any cellular system.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>173339</pmid><doi>10.1016/0006-291X(75)90171-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1975-12, Vol.67 (4), p.1319-1325 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Bucladesine - pharmacology Caffeine - pharmacology Cell Division Culture Media Cyclic GMP - analogs & derivatives Cyclic GMP - pharmacology Enzyme Induction - drug effects Methyltransferases - biosynthesis Tetrahymena pyriformis - drug effects Tetrahymena pyriformis - enzymology Theophylline - pharmacology Thymidylate Synthase - biosynthesis |
| title | Induction of thymidylate synthetase activity in Tetrahymena by cyclic guanosine monophosphate |
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