A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues
A simple procedure has been developed for the isolation of ultrastructurally pure, intact basement membranes from bovine retinal and brain blood vessels, rabbit renal tubules and rat renal glomeruli. By this procedure, cell membranes and intracellular materials are selectively solubilized with 4% so...
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| Veröffentlicht in: | Life sciences (1973) 1975-12, Vol.17 (11), p.1721-1732 |
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| container_end_page | 1732 |
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| container_issue | 11 |
| container_start_page | 1721 |
| container_title | Life sciences (1973) |
| container_volume | 17 |
| creator | Meezan, Elias Hjelle, J.Thomas Brendel, Klaus Carlson, Edward C. |
| description | A simple procedure has been developed for the isolation of ultrastructurally pure, intact basement membranes from bovine retinal and brain blood vessels, rabbit renal tubules and rat renal glomeruli. By this procedure, cell membranes and intracellular materials are selectively solubilized with 4% sodium deoxycholate to yield morphologically and chemically intact basement membrane preparations. Therefore, this method appears to be a versatile, nondisruptive procedure for the isolation and characterization of basement membranes from a variety of tissues. Its applicability has been demonstrated by the preparation for the first time of isolated basement membranes from non-renal mammalian blood vessels. |
| doi_str_mv | 10.1016/0024-3205(75)90119-8 |
| format | Article |
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By this procedure, cell membranes and intracellular materials are selectively solubilized with 4% sodium deoxycholate to yield morphologically and chemically intact basement membrane preparations. Therefore, this method appears to be a versatile, nondisruptive procedure for the isolation and characterization of basement membranes from a variety of tissues. Its applicability has been demonstrated by the preparation for the first time of isolated basement membranes from non-renal mammalian blood vessels.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(75)90119-8</identifier><identifier>PMID: 1207385</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Amino Acids - analysis ; Animals ; Basement Membrane - chemistry ; Brain - blood supply ; Brain - ultrastructure ; Carbohydrates - analysis ; Cattle ; Cell Fractionation - methods ; Deoxycholic Acid ; Kidney Glomerulus - ultrastructure ; Kidney Tubules - ultrastructure ; Microcirculation ; Microscopy, Electron ; Rabbits ; Rats ; Retinal Vessels - ultrastructure</subject><ispartof>Life sciences (1973), 1975-12, Vol.17 (11), p.1721-1732</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-ad532fdcb9f761d6896f1630797a6797aa77262a0755994a4abd1d4e1cc33bc13</citedby><cites>FETCH-LOGICAL-c423t-ad532fdcb9f761d6896f1630797a6797aa77262a0755994a4abd1d4e1cc33bc13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0024-3205(75)90119-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1207385$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meezan, Elias</creatorcontrib><creatorcontrib>Hjelle, J.Thomas</creatorcontrib><creatorcontrib>Brendel, Klaus</creatorcontrib><creatorcontrib>Carlson, Edward C.</creatorcontrib><title>A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>A simple procedure has been developed for the isolation of ultrastructurally pure, intact basement membranes from bovine retinal and brain blood vessels, rabbit renal tubules and rat renal glomeruli. By this procedure, cell membranes and intracellular materials are selectively solubilized with 4% sodium deoxycholate to yield morphologically and chemically intact basement membrane preparations. Therefore, this method appears to be a versatile, nondisruptive procedure for the isolation and characterization of basement membranes from a variety of tissues. Its applicability has been demonstrated by the preparation for the first time of isolated basement membranes from non-renal mammalian blood vessels.</description><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Basement Membrane - chemistry</subject><subject>Brain - blood supply</subject><subject>Brain - ultrastructure</subject><subject>Carbohydrates - analysis</subject><subject>Cattle</subject><subject>Cell Fractionation - methods</subject><subject>Deoxycholic Acid</subject><subject>Kidney Glomerulus - ultrastructure</subject><subject>Kidney Tubules - ultrastructure</subject><subject>Microcirculation</subject><subject>Microscopy, Electron</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Retinal Vessels - ultrastructure</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcFu1DAQtRBVWRb-ACSfEEgE7DiOk0ulqgJaqVIvcLYce8Ia2XHwOCv1F_hqEnYFNy62R-_Ne-M3hLzi7ANnvP3IWN1UombyrZLvesZ5X3VPyI53qq9YK_hTsvtLeUaeI_5gjEmpxCW55DVTopM78uuaoo9zgPf0CBlN8dtzSpPzmJe5-CPQCOWQHB1TpuUA1GMKKy9NNI00pjwfUkjfvTUhPFIzOWoPEM_lvGSgg0GIMJVVKA7ZTIB0zClShNXSBFo84gL4glyMJiC8PN978u3zp683t9X9w5e7m-v7yja1KJVxUtSjs0M_qpa7tuvbkbeCqV6ZdjuMUnVbG6ak7PvGNGZw3DXArRVisFzsyZuT7pzTz9W36OjRQgjrZGlB3YlVTtYbsTkRbU6IGUY9Zx9NftSc6W0FestXb_lqJfWfFazde_L6rL8MEdy_plPmK351wmH95NFD1mg9TBacz2CLdsn_3-A3yNCYbg</recordid><startdate>19751201</startdate><enddate>19751201</enddate><creator>Meezan, Elias</creator><creator>Hjelle, J.Thomas</creator><creator>Brendel, Klaus</creator><creator>Carlson, Edward C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19751201</creationdate><title>A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues</title><author>Meezan, Elias ; Hjelle, J.Thomas ; Brendel, Klaus ; Carlson, Edward C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-ad532fdcb9f761d6896f1630797a6797aa77262a0755994a4abd1d4e1cc33bc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Basement Membrane - chemistry</topic><topic>Brain - blood supply</topic><topic>Brain - ultrastructure</topic><topic>Carbohydrates - analysis</topic><topic>Cattle</topic><topic>Cell Fractionation - methods</topic><topic>Deoxycholic Acid</topic><topic>Kidney Glomerulus - ultrastructure</topic><topic>Kidney Tubules - ultrastructure</topic><topic>Microcirculation</topic><topic>Microscopy, Electron</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Retinal Vessels - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meezan, Elias</creatorcontrib><creatorcontrib>Hjelle, J.Thomas</creatorcontrib><creatorcontrib>Brendel, Klaus</creatorcontrib><creatorcontrib>Carlson, Edward C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meezan, Elias</au><au>Hjelle, J.Thomas</au><au>Brendel, Klaus</au><au>Carlson, Edward C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1975-12-01</date><risdate>1975</risdate><volume>17</volume><issue>11</issue><spage>1721</spage><epage>1732</epage><pages>1721-1732</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>A simple procedure has been developed for the isolation of ultrastructurally pure, intact basement membranes from bovine retinal and brain blood vessels, rabbit renal tubules and rat renal glomeruli. 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| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 1975-12, Vol.17 (11), p.1721-1732 |
| issn | 0024-3205 1879-0631 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acids - analysis Animals Basement Membrane - chemistry Brain - blood supply Brain - ultrastructure Carbohydrates - analysis Cattle Cell Fractionation - methods Deoxycholic Acid Kidney Glomerulus - ultrastructure Kidney Tubules - ultrastructure Microcirculation Microscopy, Electron Rabbits Rats Retinal Vessels - ultrastructure |
| title | A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissues |
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