Microbiologic Determination of Folic Acid Derivatives in Blood
A microbiological method for the determination of folic acid activity (F.A.A.) in blood, and serum with three organisms (L. casei, S. faecalis and P. cerevisiae), is described. The S. faecalis No. 9 strain employed shows a considerably higher sensitivity than the standard ATCC No. 8043 strain, whils...
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| Veröffentlicht in: | Blood 1962-11, Vol.20 (5), p.609-616 |
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| creator | Grossowicz, NATYHAN Mandelbaum-Shavit, Frederika Davidoff, Rosy Aronovitch, J. |
| description | A microbiological method for the determination of folic acid activity (F.A.A.) in blood, and serum with three organisms (L. casei, S. faecalis and P. cerevisiae), is described. The S. faecalis No. 9 strain employed shows a considerably higher sensitivity than the standard ATCC No. 8043 strain, whilst the specificity towards the various F.A. derivatives tested is similar. The sensitivity of the P. cerevisiae (L. citrovorum) assay is also increased by adding thymidine, which spares the requirement for folinic acid. Finally, the procedure of extraction of the F.A.A. from blood has been modified to assure release and stabilization of the compounds assayed.
F.A.A. in blood and serum of healthy adults and infants are given. |
| doi_str_mv | 10.1182/blood.V20.5.609.609 |
| format | Article |
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F.A.A. in blood and serum of healthy adults and infants are given.</description><subject>Antimetabolites</subject><subject>Biological Assay</subject><subject>Folic Acid</subject><subject>Humans</subject><subject>Leucovorin</subject><subject>Old Medline</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1962</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LwzAYgIMobk5_gSA9eWvNV9vkoDCnU2HiRb2G5ksibTOTbuC_N3UDbx5eAnmf9-sB4BzBAiGGr2TrvS7eMSzKooJ8jAMwRSVmOYQYHoIphLDKKa_RBJzE-AkhogSXx2CCCC8hI3wKbp6dCl463_oPp7I7M5jQub4ZnO8zb7Olb9P3XDmdcsFtU2JrYub67HYcfwqObNNGc7Z_Z-Btef-6eMxXLw9Pi_kqVxTiIVdcUyY5xUwjqS2xsk6bVIRVDNVUW1vxikGjGLXMkFpRlXheWiw1UqiWZAYud33XwX9tTBxE56Iybdv0xm-iYATRmqMqgWQHpqtiDMaKdXBdE74FgmLUJn61iaRNlCIpGyNVXezbb2Rn9F_N3lMCrneASUdunQkiKmd6ZbQLRg1Ce_fvgB9Hkn6A</recordid><startdate>196211</startdate><enddate>196211</enddate><creator>Grossowicz, NATYHAN</creator><creator>Mandelbaum-Shavit, Frederika</creator><creator>Davidoff, Rosy</creator><creator>Aronovitch, J.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196211</creationdate><title>Microbiologic Determination of Folic Acid Derivatives in Blood</title><author>Grossowicz, NATYHAN ; Mandelbaum-Shavit, Frederika ; Davidoff, Rosy ; Aronovitch, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-c9d48b9428d1bdf3fb743263868174dff69680ec84f8e37c4cd4895f2bd1c17b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1962</creationdate><topic>Antimetabolites</topic><topic>Biological Assay</topic><topic>Folic Acid</topic><topic>Humans</topic><topic>Leucovorin</topic><topic>Old Medline</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grossowicz, NATYHAN</creatorcontrib><creatorcontrib>Mandelbaum-Shavit, Frederika</creatorcontrib><creatorcontrib>Davidoff, Rosy</creatorcontrib><creatorcontrib>Aronovitch, J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grossowicz, NATYHAN</au><au>Mandelbaum-Shavit, Frederika</au><au>Davidoff, Rosy</au><au>Aronovitch, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbiologic Determination of Folic Acid Derivatives in Blood</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1962-11</date><risdate>1962</risdate><volume>20</volume><issue>5</issue><spage>609</spage><epage>616</epage><pages>609-616</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>A microbiological method for the determination of folic acid activity (F.A.A.) in blood, and serum with three organisms (L. casei, S. faecalis and P. cerevisiae), is described. The S. faecalis No. 9 strain employed shows a considerably higher sensitivity than the standard ATCC No. 8043 strain, whilst the specificity towards the various F.A. derivatives tested is similar. The sensitivity of the P. cerevisiae (L. citrovorum) assay is also increased by adding thymidine, which spares the requirement for folinic acid. Finally, the procedure of extraction of the F.A.A. from blood has been modified to assure release and stabilization of the compounds assayed.
F.A.A. in blood and serum of healthy adults and infants are given.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>13950839</pmid><doi>10.1182/blood.V20.5.609.609</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Blood, 1962-11, Vol.20 (5), p.609-616 |
| issn | 0006-4971 1528-0020 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antimetabolites Biological Assay Folic Acid Humans Leucovorin Old Medline |
| title | Microbiologic Determination of Folic Acid Derivatives in Blood |
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