Characterization of transcriptase and replicase particles isolated from reovirus-infected cells
Double-stranded RNA-dependent single-stranded RNA polymerase (transcriptase) and single-stranded RNA-dependent double-stranded RNA polymerase (replicase) were isolated from reovirus-infected cells. Both transcriptase and replicase were associated with subviral particles that could be separated by ve...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1975-12, Vol.68 (2), p.455-466 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Morgan, Exeen M. Zweerink, Hans J. |
| description | Double-stranded RNA-dependent single-stranded RNA polymerase (transcriptase) and single-stranded RNA-dependent double-stranded RNA polymerase (replicase) were isolated from reovirus-infected cells. Both transcriptase and replicase were associated with subviral particles that could be separated by velocity sedimentation in glycerol density gradients and by equilibrium sedimentation in CsCl density gradients. The transcriptase particles (ϱ = 1.42 g/cm
3) which sedimented at approximately 220 S contained double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ0, and σ2. The majority of the replicase particles (ϱ = 1.35 g/cm
3) sedimented at approximately 180 S. These particles were composed of single-stranded, partially double-stranded and double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ2, σ1, σ2, and σ3. Both particles were approximately 40 nm in diameter. |
| doi_str_mv | 10.1016/0042-6822(75)90286-X |
| format | Article |
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3) which sedimented at approximately 220 S contained double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ0, and σ2. The majority of the replicase particles (ϱ = 1.35 g/cm
3) sedimented at approximately 180 S. These particles were composed of single-stranded, partially double-stranded and double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ2, σ1, σ2, and σ3. Both particles were approximately 40 nm in diameter.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(75)90286-X</identifier><identifier>PMID: 1198927</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Line ; Cell-Free System ; DNA-Directed RNA Polymerases - analysis ; DNA-Directed RNA Polymerases - isolation & purification ; DNA-Directed RNA Polymerases - metabolism ; Peptides - analysis ; Reoviridae - enzymology ; Reoviridae - ultrastructure ; RNA Nucleotidyltransferases ; RNA Replicase - analysis ; RNA Replicase - isolation & purification ; RNA Replicase - metabolism ; RNA, Viral - biosynthesis</subject><ispartof>Virology (New York, N.Y.), 1975-12, Vol.68 (2), p.455-466</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-c4eeb06a7c4107ba32f5d736a6a2b7e91beabd1a30e56175d2576e5e6e5704ed3</citedby><cites>FETCH-LOGICAL-c357t-c4eeb06a7c4107ba32f5d736a6a2b7e91beabd1a30e56175d2576e5e6e5704ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(75)90286-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1198927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morgan, Exeen M.</creatorcontrib><creatorcontrib>Zweerink, Hans J.</creatorcontrib><title>Characterization of transcriptase and replicase particles isolated from reovirus-infected cells</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Double-stranded RNA-dependent single-stranded RNA polymerase (transcriptase) and single-stranded RNA-dependent double-stranded RNA polymerase (replicase) were isolated from reovirus-infected cells. Both transcriptase and replicase were associated with subviral particles that could be separated by velocity sedimentation in glycerol density gradients and by equilibrium sedimentation in CsCl density gradients. The transcriptase particles (ϱ = 1.42 g/cm
3) which sedimented at approximately 220 S contained double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ0, and σ2. The majority of the replicase particles (ϱ = 1.35 g/cm
3) sedimented at approximately 180 S. These particles were composed of single-stranded, partially double-stranded and double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ2, σ1, σ2, and σ3. Both particles were approximately 40 nm in diameter.</description><subject>Cell Line</subject><subject>Cell-Free System</subject><subject>DNA-Directed RNA Polymerases - analysis</subject><subject>DNA-Directed RNA Polymerases - isolation & purification</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Peptides - analysis</subject><subject>Reoviridae - enzymology</subject><subject>Reoviridae - ultrastructure</subject><subject>RNA Nucleotidyltransferases</subject><subject>RNA Replicase - analysis</subject><subject>RNA Replicase - isolation & purification</subject><subject>RNA Replicase - metabolism</subject><subject>RNA, Viral - biosynthesis</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo67r6DxR6Ej1Uk6ZJthdBFr9gwYvC3sI0mWKk29Sku6C_3tYuevMwDDPzztdDyCmjV4wyeU1pnqVynmUXSlwWNJvLdLVHpowWMqU8Z_tk-is5JEcxvtM-VopOyISxYl5kakr04g0CmA6D-4LO-SbxVdIFaKIJru0gYgKNTQK2tTND1ELonKkxJi76Gjq0SRX8ulf4rQubmLqmQjOkDdZ1PCYHFdQRT3Z-Rl7v714Wj-ny-eFpcbtMDReqS02OWFIJyuSMqhJ4VgmruAQJWamwYCVCaRlwikIyJWwmlESBvSmao-Uzcj7ObYP_2GDs9NrF4QJo0G-innPGBZeyF-aj0AQfY8BKt8GtIXxqRvXAVQ_Q9ABNK6F_uOpV33a2m78p12j_mkaQff1mrGP_5NZh0NE4bAxaF3oc2nr3_4JvzS2J0A</recordid><startdate>197512</startdate><enddate>197512</enddate><creator>Morgan, Exeen M.</creator><creator>Zweerink, Hans J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197512</creationdate><title>Characterization of transcriptase and replicase particles isolated from reovirus-infected cells</title><author>Morgan, Exeen M. ; Zweerink, Hans J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-c4eeb06a7c4107ba32f5d736a6a2b7e91beabd1a30e56175d2576e5e6e5704ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Cell Line</topic><topic>Cell-Free System</topic><topic>DNA-Directed RNA Polymerases - analysis</topic><topic>DNA-Directed RNA Polymerases - isolation & purification</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Peptides - analysis</topic><topic>Reoviridae - enzymology</topic><topic>Reoviridae - ultrastructure</topic><topic>RNA Nucleotidyltransferases</topic><topic>RNA Replicase - analysis</topic><topic>RNA Replicase - isolation & purification</topic><topic>RNA Replicase - metabolism</topic><topic>RNA, Viral - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morgan, Exeen M.</creatorcontrib><creatorcontrib>Zweerink, Hans J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morgan, Exeen M.</au><au>Zweerink, Hans J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of transcriptase and replicase particles isolated from reovirus-infected cells</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1975-12</date><risdate>1975</risdate><volume>68</volume><issue>2</issue><spage>455</spage><epage>466</epage><pages>455-466</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Double-stranded RNA-dependent single-stranded RNA polymerase (transcriptase) and single-stranded RNA-dependent double-stranded RNA polymerase (replicase) were isolated from reovirus-infected cells. Both transcriptase and replicase were associated with subviral particles that could be separated by velocity sedimentation in glycerol density gradients and by equilibrium sedimentation in CsCl density gradients. The transcriptase particles (ϱ = 1.42 g/cm
3) which sedimented at approximately 220 S contained double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ0, and σ2. The majority of the replicase particles (ϱ = 1.35 g/cm
3) sedimented at approximately 180 S. These particles were composed of single-stranded, partially double-stranded and double-stranded RNA and the virus-specific polypeptides λ1, λ2, μ2, σ1, σ2, and σ3. Both particles were approximately 40 nm in diameter.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>1198927</pmid><doi>10.1016/0042-6822(75)90286-X</doi><tpages>12</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1975-12, Vol.68 (2), p.455-466 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier) |
| subjects | Cell Line Cell-Free System DNA-Directed RNA Polymerases - analysis DNA-Directed RNA Polymerases - isolation & purification DNA-Directed RNA Polymerases - metabolism Peptides - analysis Reoviridae - enzymology Reoviridae - ultrastructure RNA Nucleotidyltransferases RNA Replicase - analysis RNA Replicase - isolation & purification RNA Replicase - metabolism RNA, Viral - biosynthesis |
| title | Characterization of transcriptase and replicase particles isolated from reovirus-infected cells |
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