Novel elastic material from collagen for tissue engineering

Elastic collagen gel (e-gel) was prepared from salmon atelocollagen fibrillar gel reinforced by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) mediated cross-linking (f-gel). The preparation consisted of a simple heat treatment of the f-gel at 80 degrees C, in which the f-gel drastically shran...

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Veröffentlicht in:Journal of materials science. Materials in medicine 2007-07, Vol.18 (7), p.1369-1375
Hauptverfasser: Yunoki, Shunji, Mori, Kazuo, Suzuki, Takeshi, Nagai, Nobuhiro, Munekata, Masanobu
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container_title Journal of materials science. Materials in medicine
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creator Yunoki, Shunji
Mori, Kazuo
Suzuki, Takeshi
Nagai, Nobuhiro
Munekata, Masanobu
description Elastic collagen gel (e-gel) was prepared from salmon atelocollagen fibrillar gel reinforced by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) mediated cross-linking (f-gel). The preparation consisted of a simple heat treatment of the f-gel at 80 degrees C, in which the f-gel drastically shrank and the collagen fibril structure was deformed. The e-gel obtained showed rubber-like elasticity; its stress-strain behavior little changed through repeated stretching. The elongation at the breaking point was approximately 230%. Furthermore, normal human osteoblasts showed good attachment and proliferation on the e-gel. These results suggest its potential to be utilized for the development of tissue engineering.
doi_str_mv 10.1007/s10856-007-0121-6
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subjects Biocompatible Materials - chemistry
Biomedical materials
Cell Culture Techniques - methods
Cell Proliferation
Cell Survival
Cells, Cultured
Collagen
Elasticity
Fibrillar Collagens - chemistry
Fibrillar Collagens - ultrastructure
Hardness
Humans
Materials science
Osteoblasts - cytology
Osteoblasts - physiology
Salmonidae
Stress, Mechanical
Tensile Strength
Tissue engineering
Tissue Engineering - methods
title Novel elastic material from collagen for tissue engineering
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