Effects of constant static pressure on the biological properties of porcine aortic valve leaflets
An understanding of how mechanical forces impact cells within valve leaflets would greatly benefit the development of a tissue-engineered heart valve. In this study, the effect of constant ambient pressure on the biological properties of heart valve leaflets was evaluated using a custom-designed pre...
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| Veröffentlicht in: | Annals of biomedical engineering 2004-04, Vol.32 (4), p.555-562 |
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| creator | Xing, Yun He, Zhaoming Warnock, James N Hilbert, Stephen L Yoganathan, Ajit P |
| description | An understanding of how mechanical forces impact cells within valve leaflets would greatly benefit the development of a tissue-engineered heart valve. In this study, the effect of constant ambient pressure on the biological properties of heart valve leaflets was evaluated using a custom-designed pressure system. Native porcine aortic valve leaflets were exposed to static pressures of 100, 140, or 170 mmHg for 48 h. Collagen synthesis, DNA synthesis, sulfated glycoaminoglycan (sGAG) synthesis, alpha-SMC actin expression, and extracellular matrix (ECM) structure were examined. Results showed that elevated pressure caused an increase in collagen synthesis. This increase was not statistically significant at 100 mmHg, but at 140 mmHg and 170 mmHg collagen synthesis increased by 37.5 and 90%, respectively. No significant difference in DNA or sGAG synthesis was observed at elevated pressures, with the exception that DNA synthesis at 100 mmHg decreased. A notable decline in alpha-SMC actin was observed over the course of the experiments although no significant difference was observed between the pressure and control groups. It was concluded that elevated pressure caused a proportional increase in collagen synthesis of porcine aortic valve leaflets, but was unable to preserve alpha-SMC actin immunoreactive cells. |
| doi_str_mv | 10.1023/b:abme.0000019175.12013.8f |
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In this study, the effect of constant ambient pressure on the biological properties of heart valve leaflets was evaluated using a custom-designed pressure system. Native porcine aortic valve leaflets were exposed to static pressures of 100, 140, or 170 mmHg for 48 h. Collagen synthesis, DNA synthesis, sulfated glycoaminoglycan (sGAG) synthesis, alpha-SMC actin expression, and extracellular matrix (ECM) structure were examined. Results showed that elevated pressure caused an increase in collagen synthesis. This increase was not statistically significant at 100 mmHg, but at 140 mmHg and 170 mmHg collagen synthesis increased by 37.5 and 90%, respectively. No significant difference in DNA or sGAG synthesis was observed at elevated pressures, with the exception that DNA synthesis at 100 mmHg decreased. A notable decline in alpha-SMC actin was observed over the course of the experiments although no significant difference was observed between the pressure and control groups. It was concluded that elevated pressure caused a proportional increase in collagen synthesis of porcine aortic valve leaflets, but was unable to preserve alpha-SMC actin immunoreactive cells.</description><identifier>ISSN: 0090-6964</identifier><identifier>EISSN: 1573-9686</identifier><identifier>DOI: 10.1023/b:abme.0000019175.12013.8f</identifier><identifier>PMID: 15117029</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Actins - metabolism ; Animals ; Aortic Valve - anatomy & histology ; Aortic Valve - cytology ; Aortic Valve - metabolism ; Biological ; Biological properties ; Collagen ; Collagen - biosynthesis ; Collagens ; Deoxyribonucleic acid ; DNA ; DNA - biosynthesis ; Electrochemical machining ; Elevated ; Extracellular Matrix - metabolism ; Glycosaminoglycans - biosynthesis ; Heart valves ; Immunohistochemistry ; Organ Culture Techniques ; Pressure ; Staining and Labeling ; Static pressure ; Stress, Mechanical ; Swine ; Synthesis ; Tissue Engineering ; Valve leaflets</subject><ispartof>Annals of biomedical engineering, 2004-04, Vol.32 (4), p.555-562</ispartof><rights>Biomedical Engineering Society 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-37def3003069bacd06085534e3aca300ccbc90479bfb637cf991d250fcffd0bb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15117029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xing, Yun</creatorcontrib><creatorcontrib>He, Zhaoming</creatorcontrib><creatorcontrib>Warnock, James N</creatorcontrib><creatorcontrib>Hilbert, Stephen L</creatorcontrib><creatorcontrib>Yoganathan, Ajit P</creatorcontrib><title>Effects of constant static pressure on the biological properties of porcine aortic valve leaflets</title><title>Annals of biomedical engineering</title><addtitle>Ann Biomed Eng</addtitle><description>An understanding of how mechanical forces impact cells within valve leaflets would greatly benefit the development of a tissue-engineered heart valve. In this study, the effect of constant ambient pressure on the biological properties of heart valve leaflets was evaluated using a custom-designed pressure system. Native porcine aortic valve leaflets were exposed to static pressures of 100, 140, or 170 mmHg for 48 h. Collagen synthesis, DNA synthesis, sulfated glycoaminoglycan (sGAG) synthesis, alpha-SMC actin expression, and extracellular matrix (ECM) structure were examined. Results showed that elevated pressure caused an increase in collagen synthesis. This increase was not statistically significant at 100 mmHg, but at 140 mmHg and 170 mmHg collagen synthesis increased by 37.5 and 90%, respectively. No significant difference in DNA or sGAG synthesis was observed at elevated pressures, with the exception that DNA synthesis at 100 mmHg decreased. A notable decline in alpha-SMC actin was observed over the course of the experiments although no significant difference was observed between the pressure and control groups. It was concluded that elevated pressure caused a proportional increase in collagen synthesis of porcine aortic valve leaflets, but was unable to preserve alpha-SMC actin immunoreactive cells.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Aortic Valve - anatomy & histology</subject><subject>Aortic Valve - cytology</subject><subject>Aortic Valve - metabolism</subject><subject>Biological</subject><subject>Biological properties</subject><subject>Collagen</subject><subject>Collagen - biosynthesis</subject><subject>Collagens</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - biosynthesis</subject><subject>Electrochemical machining</subject><subject>Elevated</subject><subject>Extracellular Matrix - metabolism</subject><subject>Glycosaminoglycans - biosynthesis</subject><subject>Heart valves</subject><subject>Immunohistochemistry</subject><subject>Organ Culture 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P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of constant static pressure on the biological properties of porcine aortic valve leaflets</atitle><jtitle>Annals of biomedical engineering</jtitle><addtitle>Ann Biomed Eng</addtitle><date>2004-04</date><risdate>2004</risdate><volume>32</volume><issue>4</issue><spage>555</spage><epage>562</epage><pages>555-562</pages><issn>0090-6964</issn><eissn>1573-9686</eissn><abstract>An understanding of how mechanical forces impact cells within valve leaflets would greatly benefit the development of a tissue-engineered heart valve. In this study, the effect of constant ambient pressure on the biological properties of heart valve leaflets was evaluated using a custom-designed pressure system. Native porcine aortic valve leaflets were exposed to static pressures of 100, 140, or 170 mmHg for 48 h. Collagen synthesis, DNA synthesis, sulfated glycoaminoglycan (sGAG) synthesis, alpha-SMC actin expression, and extracellular matrix (ECM) structure were examined. Results showed that elevated pressure caused an increase in collagen synthesis. This increase was not statistically significant at 100 mmHg, but at 140 mmHg and 170 mmHg collagen synthesis increased by 37.5 and 90%, respectively. No significant difference in DNA or sGAG synthesis was observed at elevated pressures, with the exception that DNA synthesis at 100 mmHg decreased. A notable decline in alpha-SMC actin was observed over the course of the experiments although no significant difference was observed between the pressure and control groups. It was concluded that elevated pressure caused a proportional increase in collagen synthesis of porcine aortic valve leaflets, but was unable to preserve alpha-SMC actin immunoreactive cells.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>15117029</pmid><doi>10.1023/b:abme.0000019175.12013.8f</doi><tpages>8</tpages></addata></record> |
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| subjects | Actins - metabolism Animals Aortic Valve - anatomy & histology Aortic Valve - cytology Aortic Valve - metabolism Biological Biological properties Collagen Collagen - biosynthesis Collagens Deoxyribonucleic acid DNA DNA - biosynthesis Electrochemical machining Elevated Extracellular Matrix - metabolism Glycosaminoglycans - biosynthesis Heart valves Immunohistochemistry Organ Culture Techniques Pressure Staining and Labeling Static pressure Stress, Mechanical Swine Synthesis Tissue Engineering Valve leaflets |
| title | Effects of constant static pressure on the biological properties of porcine aortic valve leaflets |
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