Osteoblast response and calcium deposition on phospholipid modified surfaces
In this study, the effect of calcium phosphate complexed phospholipid (Ca-PL-PO4) coatings on solid surfaces on the in vitro calcium (Ca) deposition and on the osteoblast responses was evaluated. Commercially available phospholipids were converted to their Ca-PL-PO4, and were coated on glass Petri d...
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| Veröffentlicht in: | Journal of materials science. Materials in medicine 2004-06, Vol.15 (6), p.693-697 |
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| creator | Satsangi, Neera Satsangi, Arpan Glover, Renee Ong, Joo L Satsangi, Rajiv K |
| description | In this study, the effect of calcium phosphate complexed phospholipid (Ca-PL-PO4) coatings on solid surfaces on the in vitro calcium (Ca) deposition and on the osteoblast responses was evaluated. Commercially available phospholipids were converted to their Ca-PL-PO4, and were coated on glass Petri dishes. The coated dishes were immersed in the simulated body fluid for up to 14 days under sterilized conditions at 37 degrees C, and the amount of calcium (Ca) deposited was quantified. Similarly, by measuring the alkaline phosphatase specific activity, the differentiation of osteoblast precursor cells were evaluated after seeding the cells on Ca-PL-PO4 coated cell culture plastics. It was observed that all Ca-PL-PO4 enhanced Ca deposition on coated surfaces. The, polar head group of phospholipids in coated surfaces was observed to have an influence on the Ca deposition as well as the osteoblast differentiation. Among the phospholipids evaluated, phosphatidylserine (Ca-PS-PO4) exhibited the strongest calcium deposition and more enhanced alkaline phosphatase specific activity. It was therefore concluded from this study that Ca-PS-PO4 surface modification may be an alternative method for enhancing bone-implant interactions. |
| doi_str_mv | 10.1023/B:JMSM.0000030211.32655.80 |
| format | Article |
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Commercially available phospholipids were converted to their Ca-PL-PO4, and were coated on glass Petri dishes. The coated dishes were immersed in the simulated body fluid for up to 14 days under sterilized conditions at 37 degrees C, and the amount of calcium (Ca) deposited was quantified. Similarly, by measuring the alkaline phosphatase specific activity, the differentiation of osteoblast precursor cells were evaluated after seeding the cells on Ca-PL-PO4 coated cell culture plastics. It was observed that all Ca-PL-PO4 enhanced Ca deposition on coated surfaces. The, polar head group of phospholipids in coated surfaces was observed to have an influence on the Ca deposition as well as the osteoblast differentiation. Among the phospholipids evaluated, phosphatidylserine (Ca-PS-PO4) exhibited the strongest calcium deposition and more enhanced alkaline phosphatase specific activity. It was therefore concluded from this study that Ca-PS-PO4 surface modification may be an alternative method for enhancing bone-implant interactions.</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1023/B:JMSM.0000030211.32655.80</identifier><identifier>PMID: 15346737</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Biomedical materials ; Body Fluids - chemistry ; Calcium ; Calcium - chemistry ; Calcium Phosphates - chemistry ; Cell Division ; Cell Line ; Cell Survival ; Chemical Precipitation ; Coated Materials, Biocompatible - chemistry ; Humans ; Lipids ; Materials science ; Materials Testing ; Orthopedics ; Osteoblasts - cytology ; Osteoblasts - physiology ; Phospholipids - chemistry ; Surface Properties ; Transplants & implants</subject><ispartof>Journal of materials science. 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Materials in medicine</title><addtitle>J Mater Sci Mater Med</addtitle><description>In this study, the effect of calcium phosphate complexed phospholipid (Ca-PL-PO4) coatings on solid surfaces on the in vitro calcium (Ca) deposition and on the osteoblast responses was evaluated. Commercially available phospholipids were converted to their Ca-PL-PO4, and were coated on glass Petri dishes. The coated dishes were immersed in the simulated body fluid for up to 14 days under sterilized conditions at 37 degrees C, and the amount of calcium (Ca) deposited was quantified. Similarly, by measuring the alkaline phosphatase specific activity, the differentiation of osteoblast precursor cells were evaluated after seeding the cells on Ca-PL-PO4 coated cell culture plastics. It was observed that all Ca-PL-PO4 enhanced Ca deposition on coated surfaces. The, polar head group of phospholipids in coated surfaces was observed to have an influence on the Ca deposition as well as the osteoblast differentiation. Among the phospholipids evaluated, phosphatidylserine (Ca-PS-PO4) exhibited the strongest calcium deposition and more enhanced alkaline phosphatase specific activity. It was therefore concluded from this study that Ca-PS-PO4 surface modification may be an alternative method for enhancing bone-implant interactions.</description><subject>Biomedical materials</subject><subject>Body Fluids - chemistry</subject><subject>Calcium</subject><subject>Calcium - chemistry</subject><subject>Calcium Phosphates - chemistry</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cell Survival</subject><subject>Chemical Precipitation</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Humans</subject><subject>Lipids</subject><subject>Materials science</subject><subject>Materials Testing</subject><subject>Orthopedics</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - physiology</subject><subject>Phospholipids - chemistry</subject><subject>Surface Properties</subject><subject>Transplants & implants</subject><issn>0957-4530</issn><issn>1573-4838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkstKxDAUhoMoOl5eQYoLXXVMcnKrO0e8MuJCXYc0TTHSNrVpF769GR0QXKghIRz4Tk74-RA6InhOMIXTxdnd_eP9HK8WYErIHKjgfK7wBpoRLiFnCtQmmuGCy5xxwDtoN8bXhLOC8220QzgwIUHO0PIhji6UjYljNrjYhy66zHRVZk1j_dRmletD9KMPXZZ2_xJiOo3vfZW1ofK1d1UWp6E21sV9tFWbJrqD9b2Hnq8uny5u8uXD9e3F-TK3aeqY80oIMFCTigllVcFtyQvLpBOltNhRgdP_HJGO0VRZu6pJSU2NqeKClbCHTr7e7YfwNrk46tZH65rGdC5MUSsgRBREyUQe_0oKoRgVFP4EaUqUKyH_AwrBC_YnSFL-hCiawKMf4GuYhi4FqCUXAKyAIkFnX5AdQoyDq3U_-NYM75pgvbJCL_TKCv1thf60Qiucmg_XE6ayddV361oD-ABnkLHz</recordid><startdate>200406</startdate><enddate>200406</enddate><creator>Satsangi, Neera</creator><creator>Satsangi, Arpan</creator><creator>Glover, Renee</creator><creator>Ong, Joo L</creator><creator>Satsangi, Rajiv K</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0W</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>200406</creationdate><title>Osteoblast response and calcium deposition on phospholipid modified surfaces</title><author>Satsangi, Neera ; Satsangi, Arpan ; Glover, Renee ; Ong, Joo L ; Satsangi, Rajiv K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-5d663a3f1d468c895cb59c47e6b7c0e260153e17e420e2cc60151b2af028564b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biomedical materials</topic><topic>Body Fluids - 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Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Satsangi, Neera</au><au>Satsangi, Arpan</au><au>Glover, Renee</au><au>Ong, Joo L</au><au>Satsangi, Rajiv K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteoblast response and calcium deposition on phospholipid modified surfaces</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><addtitle>J Mater Sci Mater Med</addtitle><date>2004-06</date><risdate>2004</risdate><volume>15</volume><issue>6</issue><spage>693</spage><epage>697</epage><pages>693-697</pages><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>In this study, the effect of calcium phosphate complexed phospholipid (Ca-PL-PO4) coatings on solid surfaces on the in vitro calcium (Ca) deposition and on the osteoblast responses was evaluated. Commercially available phospholipids were converted to their Ca-PL-PO4, and were coated on glass Petri dishes. The coated dishes were immersed in the simulated body fluid for up to 14 days under sterilized conditions at 37 degrees C, and the amount of calcium (Ca) deposited was quantified. Similarly, by measuring the alkaline phosphatase specific activity, the differentiation of osteoblast precursor cells were evaluated after seeding the cells on Ca-PL-PO4 coated cell culture plastics. It was observed that all Ca-PL-PO4 enhanced Ca deposition on coated surfaces. The, polar head group of phospholipids in coated surfaces was observed to have an influence on the Ca deposition as well as the osteoblast differentiation. Among the phospholipids evaluated, phosphatidylserine (Ca-PS-PO4) exhibited the strongest calcium deposition and more enhanced alkaline phosphatase specific activity. It was therefore concluded from this study that Ca-PS-PO4 surface modification may be an alternative method for enhancing bone-implant interactions.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>15346737</pmid><doi>10.1023/B:JMSM.0000030211.32655.80</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of materials science. Materials in medicine, 2004-06, Vol.15 (6), p.693-697 |
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| subjects | Biomedical materials Body Fluids - chemistry Calcium Calcium - chemistry Calcium Phosphates - chemistry Cell Division Cell Line Cell Survival Chemical Precipitation Coated Materials, Biocompatible - chemistry Humans Lipids Materials science Materials Testing Orthopedics Osteoblasts - cytology Osteoblasts - physiology Phospholipids - chemistry Surface Properties Transplants & implants |
| title | Osteoblast response and calcium deposition on phospholipid modified surfaces |
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