Methods for obtaining peptide maps of proteins on a subnanomole scale

A rapid method of peptide mapping by thin layer electrophoresis and chromatography is described that permits good maps to be produced from as little as 0.5 nmole of protein by means of conventional staining procedures with ninhydrin. The method can be made even more sensitive by prior amidination of...

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Veröffentlicht in:	Analytical biochemistry 1975-09, Vol.68 (1), p.175-184
Hauptverfasser:	Bates, David L., Perham, Richard N., Coggins, John R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Thin Layer - methods Chymotrypsin Electrophoresis - instrumentation Electrophoresis - methods Fructose-Bisphosphate Aldolase - analysis Microchemistry Peptide Fragments - isolation & purification Proteins - analysis Silicon Dioxide Trypsin
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