Identification of the UUG codon as a translational initiation codon in vivo

The lac repressor protein was purified from an Escherichia coli strain carrying an amber mutation in the lacI gene and the tyrosine-inserting amber suppressor, Su3. Protein sequencing showed a change at position 62 in the repressor polypeptide chain from leucine to tyrosine, proving that the amber w...

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Veröffentlicht in:	Journal of molecular biology 1975-06, Vol.95 (2), p.327-330
Hauptverfasser:	Files, James G., Weber, Klaus, Coulondre, Christine, Miller, Jeffrey H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - analysis Bacterial Proteins - isolation & purification Codon Escherichia coli - analysis Genes Genetics, Microbial Mutation Peptide Chain Initiation, Translational Protein Biosynthesis RNA, Messenger
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container_title	Journal of molecular biology
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creator	Files, James G. Weber, Klaus Coulondre, Christine Miller, Jeffrey H.
description	The lac repressor protein was purified from an Escherichia coli strain carrying an amber mutation in the lacI gene and the tyrosine-inserting amber suppressor, Su3. Protein sequencing showed a change at position 62 in the repressor polypeptide chain from leucine to tyrosine, proving that the amber was derived from a UUG codon at this point in the message. This establishes UUG as an initiation codon in vivo, since it has been previously shown that translational reinitiation can occur at position 62.
doi_str_mv	10.1016/0022-2836(75)90398-8
format	Article
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Protein sequencing showed a change at position 62 in the repressor polypeptide chain from leucine to tyrosine, proving that the amber was derived from a UUG codon at this point in the message. This establishes UUG as an initiation codon in vivo, since it has been previously shown that translational reinitiation can occur at position 62.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/0022-2836(75)90398-8</identifier><identifier>PMID: 1102708</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - analysis ; Bacterial Proteins - isolation & purification ; Codon ; Escherichia coli - analysis ; Genes ; Genetics, Microbial ; Mutation ; Peptide Chain Initiation, Translational ; Protein Biosynthesis ; RNA, Messenger</subject><ispartof>Journal of molecular biology, 1975-06, Vol.95 (2), p.327-330</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-d81fef877be0d72dbe2a8e7530305266bc5ca0d66776b65e1dec8564647832c63</citedby><cites>FETCH-LOGICAL-c272t-d81fef877be0d72dbe2a8e7530305266bc5ca0d66776b65e1dec8564647832c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0022283675903988$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1102708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Files, James G.</creatorcontrib><creatorcontrib>Weber, Klaus</creatorcontrib><creatorcontrib>Coulondre, Christine</creatorcontrib><creatorcontrib>Miller, Jeffrey H.</creatorcontrib><title>Identification of the UUG codon as a translational initiation codon in vivo</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>The lac repressor protein was purified from an Escherichia coli strain carrying an amber mutation in the lacI gene and the tyrosine-inserting amber suppressor, Su3. Protein sequencing showed a change at position 62 in the repressor polypeptide chain from leucine to tyrosine, proving that the amber was derived from a UUG codon at this point in the message. This establishes UUG as an initiation codon in vivo, since it has been previously shown that translational reinitiation can occur at position 62.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Codon</subject><subject>Escherichia coli - analysis</subject><subject>Genes</subject><subject>Genetics, Microbial</subject><subject>Mutation</subject><subject>Peptide Chain Initiation, Translational</subject><subject>Protein Biosynthesis</subject><subject>RNA, Messenger</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKAzEQhoMotVbfQCEn0cPqJOkm6UWQorVY8GLPIZvMYmS7W5Ntwbd32y168zQw8_0zzEfIJYM7BkzeA3CecS3kjcpvJyAmOtNHZMhATzIthT4mw1_klJyl9AkAuRjrARkwBlyBHpLXuce6DWVwtg1NTZuSth9Il8sZdY3vGjZRS9to61TtCVvRUIc29HjPhJpuw7Y5JyelrRJeHOqILJ-f3qcv2eJtNp8-LjLHFW8zr1mJpVaqQPCK-wK51ahyAQJyLmXhcmfBS6mULGSOzKPTuRzLsdKCOylG5Lrfu47N1wZTa1YhOawqW2OzSUYLkFJy1oHjHnSxSSliadYxrGz8NgzMzqHZCTI7QUblZu-wS4_I1WH_plih_wv10rr5Qz_H7sltwGiSC1g79CGia41vwv8HfgCOA3-t</recordid><startdate>19750625</startdate><enddate>19750625</enddate><creator>Files, James G.</creator><creator>Weber, Klaus</creator><creator>Coulondre, Christine</creator><creator>Miller, Jeffrey H.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750625</creationdate><title>Identification of the UUG codon as a translational initiation codon in vivo</title><author>Files, James G. ; Weber, Klaus ; Coulondre, Christine ; Miller, Jeffrey H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-d81fef877be0d72dbe2a8e7530305266bc5ca0d66776b65e1dec8564647832c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Codon</topic><topic>Escherichia coli - analysis</topic><topic>Genes</topic><topic>Genetics, Microbial</topic><topic>Mutation</topic><topic>Peptide Chain Initiation, Translational</topic><topic>Protein Biosynthesis</topic><topic>RNA, Messenger</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Files, James G.</creatorcontrib><creatorcontrib>Weber, Klaus</creatorcontrib><creatorcontrib>Coulondre, Christine</creatorcontrib><creatorcontrib>Miller, Jeffrey H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Files, James G.</au><au>Weber, Klaus</au><au>Coulondre, Christine</au><au>Miller, Jeffrey H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of the UUG codon as a translational initiation codon in vivo</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1975-06-25</date><risdate>1975</risdate><volume>95</volume><issue>2</issue><spage>327</spage><epage>330</epage><pages>327-330</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>The lac repressor protein was purified from an Escherichia coli strain carrying an amber mutation in the lacI gene and the tyrosine-inserting amber suppressor, Su3. Protein sequencing showed a change at position 62 in the repressor polypeptide chain from leucine to tyrosine, proving that the amber was derived from a UUG codon at this point in the message. This establishes UUG as an initiation codon in vivo, since it has been previously shown that translational reinitiation can occur at position 62.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>1102708</pmid><doi>10.1016/0022-2836(75)90398-8</doi><tpages>4</tpages></addata></record>
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subjects	Amino Acid Sequence Bacterial Proteins - analysis Bacterial Proteins - isolation & purification Codon Escherichia coli - analysis Genes Genetics, Microbial Mutation Peptide Chain Initiation, Translational Protein Biosynthesis RNA, Messenger
title	Identification of the UUG codon as a translational initiation codon in vivo
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