Characterization and improved separation of soybean leghemoglobins

An improved separation procedure is described for isolating five leghemoglobin components from the nodules of soybean plants. After a preliminary oxidation with ferricyanide, and separation from endogenous nicotinate at pH 9.2, the ferrileghemoglobins are separated by DEAE-cellulose chromatography u...

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Veröffentlicht in:	Biochemistry (Easton) 1975-10, Vol.14 (20), p.4444-4450
Hauptverfasser:	Appleby, C. A, Nicola, N. A, Hurrell, J. G. R, Leach S. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Binding Sites Circular Dichroism Glycine max Hemeproteins - analysis Leghemoglobin - analysis Leghemoglobin - isolation & purification Nicotinic Acids - analysis Peptide Fragments - analysis Plants - analysis Protein Binding Protein Conformation Spectrophotometry Spectrophotometry, Ultraviolet
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