Preparation and characterisation of a subviral particle of vaccinia virus containing the DNA-dependent RNA polymerase activity
A subviral particle has been obtained from purified vaccinia virus by controlled protease digestion followed by an NaCl-EDTA treatment. Digestion was carried out in the presence of a neutral detergent with papain, immobilized on nylon powder, to ensure a sequential digestion from the outside of the...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1975-11, Vol.68 (1), p.234-244 |
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| container_title | Virology (New York, N.Y.) |
| container_volume | 68 |
| creator | McCrae, M.A. Szilágyi, J.F. |
| description | A subviral particle has been obtained from purified vaccinia virus by controlled protease digestion followed by an NaCl-EDTA treatment. Digestion was carried out in the presence of a neutral detergent with papain, immobilized on nylon powder, to ensure a sequential digestion from the outside of the particle. Digestion removed 40% of the virus protein to produce a brick-shaped particle with a covering of spikes. After a defined amount of virus digestion (90 min under all conditions tested), subsequent treatment with 0.5
M NaCl in the presence of 50 m
M EDTA removed a further 25–30% of the virus protein including one of the two predominant structural polypeptides 4a. The product of this treatment was a subviral particle with a smooth featureless surface. The final subviral particle obtained contained only 19 of the 48 recognised polypeptides of the virion representing 30–35% of the virion protein. This particle retained all the DNA-dependent RNA polymerase activity of the virion. |
| doi_str_mv | 10.1016/0042-6822(75)90164-6 |
| format | Article |
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M NaCl in the presence of 50 m
M EDTA removed a further 25–30% of the virus protein including one of the two predominant structural polypeptides 4a. The product of this treatment was a subviral particle with a smooth featureless surface. The final subviral particle obtained contained only 19 of the 48 recognised polypeptides of the virion representing 30–35% of the virion protein. This particle retained all the DNA-dependent RNA polymerase activity of the virion.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(75)90164-6</identifier><identifier>PMID: 810966</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>DNA-Directed RNA Polymerases - isolation & purification ; DNA-Directed RNA Polymerases - metabolism ; Edetic Acid - pharmacology ; Papain - pharmacology ; Peptide Hydrolases - pharmacology ; Peptides - isolation & purification ; Polyethylene Glycols - pharmacology ; Sodium Chloride - pharmacology ; Vaccinia virus - analysis ; Vaccinia virus - drug effects ; Vaccinia virus - enzymology ; Viral Proteins - isolation & purification</subject><ispartof>Virology (New York, N.Y.), 1975-11, Vol.68 (1), p.234-244</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-f3acdcc7228788ded91be602c96c343af4fd3c670608c8c8fe3b53fb56f999993</citedby><cites>FETCH-LOGICAL-c356t-f3acdcc7228788ded91be602c96c343af4fd3c670608c8c8fe3b53fb56f999993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682275901646$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/810966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McCrae, M.A.</creatorcontrib><creatorcontrib>Szilágyi, J.F.</creatorcontrib><title>Preparation and characterisation of a subviral particle of vaccinia virus containing the DNA-dependent RNA polymerase activity</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>A subviral particle has been obtained from purified vaccinia virus by controlled protease digestion followed by an NaCl-EDTA treatment. Digestion was carried out in the presence of a neutral detergent with papain, immobilized on nylon powder, to ensure a sequential digestion from the outside of the particle. Digestion removed 40% of the virus protein to produce a brick-shaped particle with a covering of spikes. After a defined amount of virus digestion (90 min under all conditions tested), subsequent treatment with 0.5
M NaCl in the presence of 50 m
M EDTA removed a further 25–30% of the virus protein including one of the two predominant structural polypeptides 4a. The product of this treatment was a subviral particle with a smooth featureless surface. The final subviral particle obtained contained only 19 of the 48 recognised polypeptides of the virion representing 30–35% of the virion protein. This particle retained all the DNA-dependent RNA polymerase activity of the virion.</description><subject>DNA-Directed RNA Polymerases - isolation & purification</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Edetic Acid - pharmacology</subject><subject>Papain - pharmacology</subject><subject>Peptide Hydrolases - pharmacology</subject><subject>Peptides - isolation & purification</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Sodium Chloride - pharmacology</subject><subject>Vaccinia virus - analysis</subject><subject>Vaccinia virus - drug effects</subject><subject>Vaccinia virus - enzymology</subject><subject>Viral Proteins - isolation & purification</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UE1P3DAQtSooLLT_gINPCA4BJ06c5FJptaUtEgJUtWfLGY-LUdZJbWelvfDb6zSII_bBeh_zRn6EnOXsKme5uGasLDLRFMVFXV22iSkz8YGsctaKjPEyPyCrN8sxOQnhmSVc1-yIfGxml1iRl0ePo_Iq2sFR5TSFp4QgordhIQdDFQ1Tt7Ne9TR5o4UeZ3qnAKyziiZpChQGF1XC7g-NT0i_3q8zjSM6jS7Sn_drOg79foteBaRpg93ZuP9EDo3qA35-fU_J7283vzY_sruH77eb9V0GvBIxM1yBBqiLoqmbRqNu8w4FK6AVwEuuTGk0B1EzwRpI1yDvKm66Sph2PvyUnC-5ox_-Thii3NoA2PfK4TAF2XDGcy5mY7kYwQ8heDRy9Har_F7mTM6ty7lSOVcq60r-b12KNHb2mj91W9RvQ0vNSf6yyJj-uLPoZQCLDlBbjxClHuz7-f8AYKSTfw</recordid><startdate>197511</startdate><enddate>197511</enddate><creator>McCrae, M.A.</creator><creator>Szilágyi, J.F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197511</creationdate><title>Preparation and characterisation of a subviral particle of vaccinia virus containing the DNA-dependent RNA polymerase activity</title><author>McCrae, M.A. ; Szilágyi, J.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-f3acdcc7228788ded91be602c96c343af4fd3c670608c8c8fe3b53fb56f999993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>DNA-Directed RNA Polymerases - isolation & purification</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Edetic Acid - pharmacology</topic><topic>Papain - pharmacology</topic><topic>Peptide Hydrolases - pharmacology</topic><topic>Peptides - isolation & purification</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Sodium Chloride - pharmacology</topic><topic>Vaccinia virus - analysis</topic><topic>Vaccinia virus - drug effects</topic><topic>Vaccinia virus - enzymology</topic><topic>Viral Proteins - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McCrae, M.A.</creatorcontrib><creatorcontrib>Szilágyi, J.F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McCrae, M.A.</au><au>Szilágyi, J.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and characterisation of a subviral particle of vaccinia virus containing the DNA-dependent RNA polymerase activity</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1975-11</date><risdate>1975</risdate><volume>68</volume><issue>1</issue><spage>234</spage><epage>244</epage><pages>234-244</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>A subviral particle has been obtained from purified vaccinia virus by controlled protease digestion followed by an NaCl-EDTA treatment. Digestion was carried out in the presence of a neutral detergent with papain, immobilized on nylon powder, to ensure a sequential digestion from the outside of the particle. Digestion removed 40% of the virus protein to produce a brick-shaped particle with a covering of spikes. After a defined amount of virus digestion (90 min under all conditions tested), subsequent treatment with 0.5
M NaCl in the presence of 50 m
M EDTA removed a further 25–30% of the virus protein including one of the two predominant structural polypeptides 4a. The product of this treatment was a subviral particle with a smooth featureless surface. The final subviral particle obtained contained only 19 of the 48 recognised polypeptides of the virion representing 30–35% of the virion protein. This particle retained all the DNA-dependent RNA polymerase activity of the virion.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>810966</pmid><doi>10.1016/0042-6822(75)90164-6</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1975-11, Vol.68 (1), p.234-244 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83031369 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | DNA-Directed RNA Polymerases - isolation & purification DNA-Directed RNA Polymerases - metabolism Edetic Acid - pharmacology Papain - pharmacology Peptide Hydrolases - pharmacology Peptides - isolation & purification Polyethylene Glycols - pharmacology Sodium Chloride - pharmacology Vaccinia virus - analysis Vaccinia virus - drug effects Vaccinia virus - enzymology Viral Proteins - isolation & purification |
| title | Preparation and characterisation of a subviral particle of vaccinia virus containing the DNA-dependent RNA polymerase activity |
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