Enumerating Immunoglobulin-Secreting Cells Among Peripheral Human Lymphocytes. A Hemolytic Plaque Assay for A B Cell Function

Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated...

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Veröffentlicht in:	The Journal of immunology (1950) 1975-12, Vol.115 (6), p.1700-1703
Hauptverfasser:	Eby, William C, Chong, Christine A, Dray, Sheldon, Molinaro, Giuseppe A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - isolation & purification Antibody-Producing Cells - immunology B-Lymphocytes - immunology Complement System Proteins Erythrocytes - immunology Hemolytic Plaque Technique Humans Immunoglobulin G - isolation & purification Immunoglobulins - biosynthesis Rabbits Sheep
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