Enumerating Immunoglobulin-Secreting Cells Among Peripheral Human Lymphocytes. A Hemolytic Plaque Assay for A B Cell Function
Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated...
Gespeichert in:
| Veröffentlicht in: | The Journal of immunology (1950) 1975-12, Vol.115 (6), p.1700-1703 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1703 |
|---|---|
| container_issue | 6 |
| container_start_page | 1700 |
| container_title | The Journal of immunology (1950) |
| container_volume | 115 |
| creator | Eby, William C Chong, Christine A Dray, Sheldon Molinaro, Giuseppe A |
| description | Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated in gels together with erythrocytes coated with purified antibody to human Ig. After treatment with anti-Ig antibody and complement, the Ig-secreting cells formed hemolytic plaques. In 10 normal individuals, 1.2 +/- 0.4% of the plated mononuclear cells formed plaques; in an individual tested on 7 different days during 3 months, 1.1 +/- 0.2% of the cells formed plaques. From the number of mononuclear cells in the blood, we calculated 22 +/- 8 Ig-secreting cells per mm3 of blood. Enumerating Ig-secreting cells is a new direct assay for a B cell function and need not correlate with quantitating serum Ig. Thus, this assay should be useful for studying Ig-secretion in normal and altered states. |
| doi_str_mv | 10.4049/jimmunol.115.6.1700 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_83020955</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>83020955</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-160531d2ddc57c8efa3d093fab896abc90e5569af9bbfca9590a94b5451514d93</originalsourceid><addsrcrecordid>eNpNkEFr2zAYhsVo6dJuv2AMdOpO9j7Zlhwds5A2hcAKbc9CluVERbIyySb4sP9eNWlpTxK87_vw8SD0g0BeQcV_Pxvnxt7bnBCas5zUAF_QjFAKGWPAztAMoCgyUrP6K7qM8RkAGBTVBbogBApGYIb-r_rR6SAH02_x3ZG3tb4ZremzB62CPgZLbW3EC-fT_14Hs9-licXr0ckebya333k1DTrmeIHX2nk7DUbheyv_jRovYpQT7nxI4Z8jCt-MvRqM77-h807aqL-_vVfo6Wb1uFxnm7-3d8vFJlNlXQ8ZYUBL0hZtq2it5rqTZQu87GQz50w2ioOmlHHZ8abplOSUg-RVQytKKKlaXl6h6xN3H3w6KQ7CmajSJbLXfoxiXkIBnNJULE9FFXyMQXdiH4yTYRIExKt08S5dJOmCiVfpafXzDT82Trcfm5PllP865Tuz3R1M0CI6aW1qE3E4HD6RXgATtY6_</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83020955</pqid></control><display><type>article</type><title>Enumerating Immunoglobulin-Secreting Cells Among Peripheral Human Lymphocytes. A Hemolytic Plaque Assay for A B Cell Function</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Eby, William C ; Chong, Christine A ; Dray, Sheldon ; Molinaro, Giuseppe A</creator><creatorcontrib>Eby, William C ; Chong, Christine A ; Dray, Sheldon ; Molinaro, Giuseppe A</creatorcontrib><description>Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated in gels together with erythrocytes coated with purified antibody to human Ig. After treatment with anti-Ig antibody and complement, the Ig-secreting cells formed hemolytic plaques. In 10 normal individuals, 1.2 +/- 0.4% of the plated mononuclear cells formed plaques; in an individual tested on 7 different days during 3 months, 1.1 +/- 0.2% of the cells formed plaques. From the number of mononuclear cells in the blood, we calculated 22 +/- 8 Ig-secreting cells per mm3 of blood. Enumerating Ig-secreting cells is a new direct assay for a B cell function and need not correlate with quantitating serum Ig. Thus, this assay should be useful for studying Ig-secretion in normal and altered states.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.115.6.1700</identifier><identifier>PMID: 1102610</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Animals ; Antibodies - isolation & purification ; Antibody-Producing Cells - immunology ; B-Lymphocytes - immunology ; Complement System Proteins ; Erythrocytes - immunology ; Hemolytic Plaque Technique ; Humans ; Immunoglobulin G - isolation & purification ; Immunoglobulins - biosynthesis ; Rabbits ; Sheep</subject><ispartof>The Journal of immunology (1950), 1975-12, Vol.115 (6), p.1700-1703</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-160531d2ddc57c8efa3d093fab896abc90e5569af9bbfca9590a94b5451514d93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1102610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eby, William C</creatorcontrib><creatorcontrib>Chong, Christine A</creatorcontrib><creatorcontrib>Dray, Sheldon</creatorcontrib><creatorcontrib>Molinaro, Giuseppe A</creatorcontrib><title>Enumerating Immunoglobulin-Secreting Cells Among Peripheral Human Lymphocytes. A Hemolytic Plaque Assay for A B Cell Function</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated in gels together with erythrocytes coated with purified antibody to human Ig. After treatment with anti-Ig antibody and complement, the Ig-secreting cells formed hemolytic plaques. In 10 normal individuals, 1.2 +/- 0.4% of the plated mononuclear cells formed plaques; in an individual tested on 7 different days during 3 months, 1.1 +/- 0.2% of the cells formed plaques. From the number of mononuclear cells in the blood, we calculated 22 +/- 8 Ig-secreting cells per mm3 of blood. Enumerating Ig-secreting cells is a new direct assay for a B cell function and need not correlate with quantitating serum Ig. Thus, this assay should be useful for studying Ig-secretion in normal and altered states.</description><subject>Animals</subject><subject>Antibodies - isolation & purification</subject><subject>Antibody-Producing Cells - immunology</subject><subject>B-Lymphocytes - immunology</subject><subject>Complement System Proteins</subject><subject>Erythrocytes - immunology</subject><subject>Hemolytic Plaque Technique</subject><subject>Humans</subject><subject>Immunoglobulin G - isolation & purification</subject><subject>Immunoglobulins - biosynthesis</subject><subject>Rabbits</subject><subject>Sheep</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEFr2zAYhsVo6dJuv2AMdOpO9j7Zlhwds5A2hcAKbc9CluVERbIyySb4sP9eNWlpTxK87_vw8SD0g0BeQcV_Pxvnxt7bnBCas5zUAF_QjFAKGWPAztAMoCgyUrP6K7qM8RkAGBTVBbogBApGYIb-r_rR6SAH02_x3ZG3tb4ZremzB62CPgZLbW3EC-fT_14Hs9-licXr0ckebya333k1DTrmeIHX2nk7DUbheyv_jRovYpQT7nxI4Z8jCt-MvRqM77-h807aqL-_vVfo6Wb1uFxnm7-3d8vFJlNlXQ8ZYUBL0hZtq2it5rqTZQu87GQz50w2ioOmlHHZ8abplOSUg-RVQytKKKlaXl6h6xN3H3w6KQ7CmajSJbLXfoxiXkIBnNJULE9FFXyMQXdiH4yTYRIExKt08S5dJOmCiVfpafXzDT82Trcfm5PllP865Tuz3R1M0CI6aW1qE3E4HD6RXgATtY6_</recordid><startdate>197512</startdate><enddate>197512</enddate><creator>Eby, William C</creator><creator>Chong, Christine A</creator><creator>Dray, Sheldon</creator><creator>Molinaro, Giuseppe A</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197512</creationdate><title>Enumerating Immunoglobulin-Secreting Cells Among Peripheral Human Lymphocytes. A Hemolytic Plaque Assay for A B Cell Function</title><author>Eby, William C ; Chong, Christine A ; Dray, Sheldon ; Molinaro, Giuseppe A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-160531d2ddc57c8efa3d093fab896abc90e5569af9bbfca9590a94b5451514d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Antibodies - isolation & purification</topic><topic>Antibody-Producing Cells - immunology</topic><topic>B-Lymphocytes - immunology</topic><topic>Complement System Proteins</topic><topic>Erythrocytes - immunology</topic><topic>Hemolytic Plaque Technique</topic><topic>Humans</topic><topic>Immunoglobulin G - isolation & purification</topic><topic>Immunoglobulins - biosynthesis</topic><topic>Rabbits</topic><topic>Sheep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eby, William C</creatorcontrib><creatorcontrib>Chong, Christine A</creatorcontrib><creatorcontrib>Dray, Sheldon</creatorcontrib><creatorcontrib>Molinaro, Giuseppe A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eby, William C</au><au>Chong, Christine A</au><au>Dray, Sheldon</au><au>Molinaro, Giuseppe A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enumerating Immunoglobulin-Secreting Cells Among Peripheral Human Lymphocytes. A Hemolytic Plaque Assay for A B Cell Function</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1975-12</date><risdate>1975</risdate><volume>115</volume><issue>6</issue><spage>1700</spage><epage>1703</epage><pages>1700-1703</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Cells bearing immunoglobulin (Ig) can be enumerated by current methods, but cells secreting Ig (with unknown antibody specificity) can not. By using a reverse plaque-forming cell assay, we counted the Ig-secreting cells in the blood of nonimmunized individuals. Isolated human lymphocytes were plated in gels together with erythrocytes coated with purified antibody to human Ig. After treatment with anti-Ig antibody and complement, the Ig-secreting cells formed hemolytic plaques. In 10 normal individuals, 1.2 +/- 0.4% of the plated mononuclear cells formed plaques; in an individual tested on 7 different days during 3 months, 1.1 +/- 0.2% of the cells formed plaques. From the number of mononuclear cells in the blood, we calculated 22 +/- 8 Ig-secreting cells per mm3 of blood. Enumerating Ig-secreting cells is a new direct assay for a B cell function and need not correlate with quantitating serum Ig. Thus, this assay should be useful for studying Ig-secretion in normal and altered states.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>1102610</pmid><doi>10.4049/jimmunol.115.6.1700</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1975-12, Vol.115 (6), p.1700-1703 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83020955 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibodies - isolation & purification Antibody-Producing Cells - immunology B-Lymphocytes - immunology Complement System Proteins Erythrocytes - immunology Hemolytic Plaque Technique Humans Immunoglobulin G - isolation & purification Immunoglobulins - biosynthesis Rabbits Sheep |
| title | Enumerating Immunoglobulin-Secreting Cells Among Peripheral Human Lymphocytes. A Hemolytic Plaque Assay for A B Cell Function |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T09%3A43%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enumerating%20Immunoglobulin-Secreting%20Cells%20Among%20Peripheral%20Human%20Lymphocytes.%20A%20Hemolytic%20Plaque%20Assay%20for%20A%20B%20Cell%20Function&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Eby,%20William%20C&rft.date=1975-12&rft.volume=115&rft.issue=6&rft.spage=1700&rft.epage=1703&rft.pages=1700-1703&rft.issn=0022-1767&rft.eissn=1550-6606&rft_id=info:doi/10.4049/jimmunol.115.6.1700&rft_dat=%3Cproquest_cross%3E83020955%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=83020955&rft_id=info:pmid/1102610&rfr_iscdi=true |