The interaction of calcium and magnesium ions with deoxyribonucleic acid
Conductance and equilibrium dialysis studies are reported for the aqueous systems (native calf thymus) DNA-CaCl 2 and DNA-MgCl 2 at various pH values and ionic strengths at 25 °C. Discontinuities occur in the conductance curves at mole ratios of Ca 2+ and Mg 2+ to nucleate phosphorus of 0.125, 0.30,...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1975-07, Vol.169 (1), p.237-243 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Mathieson, A.R. Olayemi, J.Y. |
| description | Conductance and equilibrium dialysis studies are reported for the aqueous systems (native calf thymus) DNA-CaCl
2 and DNA-MgCl
2 at various pH values and ionic strengths at 25 °C. Discontinuities occur in the conductance curves at mole ratios of Ca
2+ and Mg
2+ to nucleate phosphorus of 0.125, 0.30, and 0.50. The dialysis results show the formation of complexes of stoichiometry 0.50 and 1.00 mol Ca
2+ or Mg
2+/mol nucleate phosphorus (2:1 and 1:1 complexes), the latter only in neutral or alkaline solutions, in agreement with the conductance discontinuity at 0.50. The other discontinuities may be due to preferential binding in the formation of the 2:1 complex. Binding constants for the 2:1 complexes are evaluated. Absorption-temperature profiles have been determined for “native” and dialysed DNA in the presence of NaCl, CaCl
2, and MgCl
2. For dialysed DNA at 26 ° C and 260 nm the decrease of absorbance with increased salt concentration was halted for MgCl
2 and CaCl
2 at a concentration corresponding to the formation of the 2:1 complex. The absorbance of “native” DNA did not decrease.
T
m
and the reciprocal of the hypochromic rise (
1
h
) increased linearly with log (salt concn). Values of
T
m
were the same at 230, 260, and 280 nm, but
h was greater at 230 and 280 than at 260 nm, which may be due to the existence of alternating blocks of (A + T) and (G − C) pairs. The entropy of transition was in the order Ca > Mg ⪢ Na. |
| doi_str_mv | 10.1016/0003-9861(75)90337-9 |
| format | Article |
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2 and DNA-MgCl
2 at various pH values and ionic strengths at 25 °C. Discontinuities occur in the conductance curves at mole ratios of Ca
2+ and Mg
2+ to nucleate phosphorus of 0.125, 0.30, and 0.50. The dialysis results show the formation of complexes of stoichiometry 0.50 and 1.00 mol Ca
2+ or Mg
2+/mol nucleate phosphorus (2:1 and 1:1 complexes), the latter only in neutral or alkaline solutions, in agreement with the conductance discontinuity at 0.50. The other discontinuities may be due to preferential binding in the formation of the 2:1 complex. Binding constants for the 2:1 complexes are evaluated. Absorption-temperature profiles have been determined for “native” and dialysed DNA in the presence of NaCl, CaCl
2, and MgCl
2. For dialysed DNA at 26 ° C and 260 nm the decrease of absorbance with increased salt concentration was halted for MgCl
2 and CaCl
2 at a concentration corresponding to the formation of the 2:1 complex. The absorbance of “native” DNA did not decrease.
T
m
and the reciprocal of the hypochromic rise (
1
h
) increased linearly with log (salt concn). Values of
T
m
were the same at 230, 260, and 280 nm, but
h was greater at 230 and 280 than at 260 nm, which may be due to the existence of alternating blocks of (A + T) and (G − C) pairs. The entropy of transition was in the order Ca > Mg ⪢ Na.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(75)90337-9</identifier><identifier>PMID: 240314</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Binding Sites ; Calcium - metabolism ; Calcium - pharmacology ; Cattle ; Conductometry ; Dialysis ; DNA - metabolism ; Hydrogen-Ion Concentration ; Kinetics ; Magnesium - metabolism ; Magnesium - pharmacology ; Nucleic Acid Conformation - drug effects ; Nucleic Acid Denaturation - drug effects ; Nucleic Acid Renaturation - drug effects ; Organophosphorus Compounds - metabolism ; Sodium Chloride - pharmacology ; Thermodynamics</subject><ispartof>Archives of biochemistry and biophysics, 1975-07, Vol.169 (1), p.237-243</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-cd7f169c46c0563818d9e2eef6cfe68213ca00ce519aceb14701673feb9e8af13</citedby><cites>FETCH-LOGICAL-c356t-cd7f169c46c0563818d9e2eef6cfe68213ca00ce519aceb14701673feb9e8af13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(75)90337-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/240314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mathieson, A.R.</creatorcontrib><creatorcontrib>Olayemi, J.Y.</creatorcontrib><title>The interaction of calcium and magnesium ions with deoxyribonucleic acid</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Conductance and equilibrium dialysis studies are reported for the aqueous systems (native calf thymus) DNA-CaCl
2 and DNA-MgCl
2 at various pH values and ionic strengths at 25 °C. Discontinuities occur in the conductance curves at mole ratios of Ca
2+ and Mg
2+ to nucleate phosphorus of 0.125, 0.30, and 0.50. The dialysis results show the formation of complexes of stoichiometry 0.50 and 1.00 mol Ca
2+ or Mg
2+/mol nucleate phosphorus (2:1 and 1:1 complexes), the latter only in neutral or alkaline solutions, in agreement with the conductance discontinuity at 0.50. The other discontinuities may be due to preferential binding in the formation of the 2:1 complex. Binding constants for the 2:1 complexes are evaluated. Absorption-temperature profiles have been determined for “native” and dialysed DNA in the presence of NaCl, CaCl
2, and MgCl
2. For dialysed DNA at 26 ° C and 260 nm the decrease of absorbance with increased salt concentration was halted for MgCl
2 and CaCl
2 at a concentration corresponding to the formation of the 2:1 complex. The absorbance of “native” DNA did not decrease.
T
m
and the reciprocal of the hypochromic rise (
1
h
) increased linearly with log (salt concn). Values of
T
m
were the same at 230, 260, and 280 nm, but
h was greater at 230 and 280 than at 260 nm, which may be due to the existence of alternating blocks of (A + T) and (G − C) pairs. The entropy of transition was in the order Ca > Mg ⪢ Na.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Calcium - metabolism</subject><subject>Calcium - pharmacology</subject><subject>Cattle</subject><subject>Conductometry</subject><subject>Dialysis</subject><subject>DNA - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Magnesium - metabolism</subject><subject>Magnesium - pharmacology</subject><subject>Nucleic Acid Conformation - drug effects</subject><subject>Nucleic Acid Denaturation - drug effects</subject><subject>Nucleic Acid Renaturation - drug effects</subject><subject>Organophosphorus Compounds - metabolism</subject><subject>Sodium Chloride - pharmacology</subject><subject>Thermodynamics</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOwzAUhi3ErRTeoEMmBEPAjhPHXpBQBRSpEkuZLefkhBolcbEToG9PQitGpqOj_yL9HyEzRm8YZeKWUspjJQW7yrNrRTnPY3VAJowqEVMu00My-bOckrMQ3illLBXJCTlOUspZOiGL1Roj23boDXTWtZGrIjA12L6JTFtGjXlrMYzfIIboy3brqET3vfW2cG0PNVqIDNjynBxVpg54sb9T8vr4sJov4uXL0_P8fhkDz0QXQ5lXTChIBdBMcMlkqTBBrARUKGTCOBhKATOmDGDB0nxYmvMKC4XSVIxPyeWud-PdR4-h040NgHVtWnR90JIPmzMpB2O6M4J3IXis9MbbxvitZlSP_PQIR49wdJ7pX35aDbHZvr8vGiz_Qjtgg3y3k3HY-GnR6wAWW8DSeoROl87-3_8DPlV_cw</recordid><startdate>197507</startdate><enddate>197507</enddate><creator>Mathieson, A.R.</creator><creator>Olayemi, J.Y.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197507</creationdate><title>The interaction of calcium and magnesium ions with deoxyribonucleic acid</title><author>Mathieson, A.R. ; Olayemi, J.Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-cd7f169c46c0563818d9e2eef6cfe68213ca00ce519aceb14701673feb9e8af13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Calcium - metabolism</topic><topic>Calcium - pharmacology</topic><topic>Cattle</topic><topic>Conductometry</topic><topic>Dialysis</topic><topic>DNA - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Magnesium - metabolism</topic><topic>Magnesium - pharmacology</topic><topic>Nucleic Acid Conformation - drug effects</topic><topic>Nucleic Acid Denaturation - drug effects</topic><topic>Nucleic Acid Renaturation - drug effects</topic><topic>Organophosphorus Compounds - metabolism</topic><topic>Sodium Chloride - pharmacology</topic><topic>Thermodynamics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mathieson, A.R.</creatorcontrib><creatorcontrib>Olayemi, J.Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mathieson, A.R.</au><au>Olayemi, J.Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The interaction of calcium and magnesium ions with deoxyribonucleic acid</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1975-07</date><risdate>1975</risdate><volume>169</volume><issue>1</issue><spage>237</spage><epage>243</epage><pages>237-243</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Conductance and equilibrium dialysis studies are reported for the aqueous systems (native calf thymus) DNA-CaCl
2 and DNA-MgCl
2 at various pH values and ionic strengths at 25 °C. Discontinuities occur in the conductance curves at mole ratios of Ca
2+ and Mg
2+ to nucleate phosphorus of 0.125, 0.30, and 0.50. The dialysis results show the formation of complexes of stoichiometry 0.50 and 1.00 mol Ca
2+ or Mg
2+/mol nucleate phosphorus (2:1 and 1:1 complexes), the latter only in neutral or alkaline solutions, in agreement with the conductance discontinuity at 0.50. The other discontinuities may be due to preferential binding in the formation of the 2:1 complex. Binding constants for the 2:1 complexes are evaluated. Absorption-temperature profiles have been determined for “native” and dialysed DNA in the presence of NaCl, CaCl
2, and MgCl
2. For dialysed DNA at 26 ° C and 260 nm the decrease of absorbance with increased salt concentration was halted for MgCl
2 and CaCl
2 at a concentration corresponding to the formation of the 2:1 complex. The absorbance of “native” DNA did not decrease.
T
m
and the reciprocal of the hypochromic rise (
1
h
) increased linearly with log (salt concn). Values of
T
m
were the same at 230, 260, and 280 nm, but
h was greater at 230 and 280 than at 260 nm, which may be due to the existence of alternating blocks of (A + T) and (G − C) pairs. The entropy of transition was in the order Ca > Mg ⪢ Na.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>240314</pmid><doi>10.1016/0003-9861(75)90337-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1975-07, Vol.169 (1), p.237-243 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83000588 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Binding Sites Calcium - metabolism Calcium - pharmacology Cattle Conductometry Dialysis DNA - metabolism Hydrogen-Ion Concentration Kinetics Magnesium - metabolism Magnesium - pharmacology Nucleic Acid Conformation - drug effects Nucleic Acid Denaturation - drug effects Nucleic Acid Renaturation - drug effects Organophosphorus Compounds - metabolism Sodium Chloride - pharmacology Thermodynamics |
| title | The interaction of calcium and magnesium ions with deoxyribonucleic acid |
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