The specific and endogenous mitotic inhibitor of lymphocytes (chalone)
Aqueous extracts of various lymphoid tissues, but not of non-lymphoid tissues, contain a species-non-specific but cell-specific inhibitor of the transformation and DNA synthesis of PHA-stimulated human lymphocytes which is apparently not cytotoxic and is reversible. This activity is found in similar...
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| Veröffentlicht in: | Experimental cell research 1975-07, Vol.93 (2), p.283-292 |
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| container_title | Experimental cell research |
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| creator | Attallah, A.M. Sunshine, G.H. Hunt, C.V. Houck, J.C. |
| description | Aqueous extracts of various lymphoid tissues, but not of non-lymphoid tissues, contain a species-non-specific but cell-specific inhibitor of the transformation and DNA synthesis of PHA-stimulated human lymphocytes which is apparently not cytotoxic and is reversible. This activity is found in similar molecular weight fractions from pure lymphocytes obtained in culture and hence appears to be endogenous to the lymphocyte itself. This specific and endogenous mitotic inhibitor does not appear to be a result of competitive lectin-binding, thymidine pool size dilution, phosphorylation, destruction of thymidine, or the direct immunosuppressive effects of thymidine upon the lymphocytes themselves. Rather, it appears to be a result of the effects of a protein contained in the crude ultrafiltrate from lymphoid tissues whose properties correspond to those originally described by Bullough & Laurence for a ‘chalone’. The chalone activity from thymus appears to be specific for T cells rather than B cells. |
| doi_str_mv | 10.1016/0014-4827(75)90452-8 |
| format | Article |
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This activity is found in similar molecular weight fractions from pure lymphocytes obtained in culture and hence appears to be endogenous to the lymphocyte itself. This specific and endogenous mitotic inhibitor does not appear to be a result of competitive lectin-binding, thymidine pool size dilution, phosphorylation, destruction of thymidine, or the direct immunosuppressive effects of thymidine upon the lymphocytes themselves. Rather, it appears to be a result of the effects of a protein contained in the crude ultrafiltrate from lymphoid tissues whose properties correspond to those originally described by Bullough & Laurence for a ‘chalone’. 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This activity is found in similar molecular weight fractions from pure lymphocytes obtained in culture and hence appears to be endogenous to the lymphocyte itself. This specific and endogenous mitotic inhibitor does not appear to be a result of competitive lectin-binding, thymidine pool size dilution, phosphorylation, destruction of thymidine, or the direct immunosuppressive effects of thymidine upon the lymphocytes themselves. Rather, it appears to be a result of the effects of a protein contained in the crude ultrafiltrate from lymphoid tissues whose properties correspond to those originally described by Bullough & Laurence for a ‘chalone’. The chalone activity from thymus appears to be specific for T cells rather than B cells.</description><subject>Animals</subject><subject>B-Lymphocytes - metabolism</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow Cells</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>DNA - biosynthesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fibroblasts - metabolism</subject><subject>Growth Inhibitors - isolation & purification</subject><subject>Growth Inhibitors - pharmacology</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Lectins</subject><subject>Lung - analysis</subject><subject>Lymph Nodes - analysis</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Mice</subject><subject>Muscles - analysis</subject><subject>Species Specificity</subject><subject>Spleen - analysis</subject><subject>T-Lymphocytes - metabolism</subject><subject>Thymidine - pharmacology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS3EqxT-oIusULsIjF-Js0FCFQWkSmzK2kqcCTFK4mKnSP170odgx2pGc-9czRxCJhTuKNDkHoCKWCiWTlM5y0BIFqsTMqKQQcwEY6dk9Gu5JFchfAKAUjS5IOeUyUTyEVmsaozCGo2trInyroywK90Hdm4Totb2rh_GtqttMfQ-clXUbNt17cy2xxBNTZ03rsPZNTmr8ibgzbGOyfviaTV_iZdvz6_zx2VsuEz6WAmTiYwy4AnjlaSclsZURWGoKUWR51LIhKsKAA0o5AVAiRVwxUFAIlHwMbk95K69-9pg6HVrg8GmyTscLtaKZTJlMh2M4mA03oXgsdJrb9vcbzUFvaOnd2j0Do1Opd7T02pYmxzzN0WL5d_SHtcgPxxkHH78tuh1MBY7g6X1aHpdOvt__g8J1H2b</recordid><startdate>197507</startdate><enddate>197507</enddate><creator>Attallah, A.M.</creator><creator>Sunshine, G.H.</creator><creator>Hunt, C.V.</creator><creator>Houck, J.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197507</creationdate><title>The specific and endogenous mitotic inhibitor of lymphocytes (chalone)</title><author>Attallah, A.M. ; Sunshine, G.H. ; Hunt, C.V. ; Houck, J.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-84c9491203623f5131dccfbbc1cd4baa545638f00ec08e3b00def038304065e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>B-Lymphocytes - metabolism</topic><topic>Bone Marrow - metabolism</topic><topic>Bone Marrow Cells</topic><topic>Cattle</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>DNA - biosynthesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fibroblasts - metabolism</topic><topic>Growth Inhibitors - isolation & purification</topic><topic>Growth Inhibitors - pharmacology</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Lectins</topic><topic>Lung - analysis</topic><topic>Lymph Nodes - analysis</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Mice</topic><topic>Muscles - analysis</topic><topic>Species Specificity</topic><topic>Spleen - analysis</topic><topic>T-Lymphocytes - metabolism</topic><topic>Thymidine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Attallah, A.M.</creatorcontrib><creatorcontrib>Sunshine, G.H.</creatorcontrib><creatorcontrib>Hunt, C.V.</creatorcontrib><creatorcontrib>Houck, J.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Attallah, A.M.</au><au>Sunshine, G.H.</au><au>Hunt, C.V.</au><au>Houck, J.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The specific and endogenous mitotic inhibitor of lymphocytes (chalone)</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1975-07</date><risdate>1975</risdate><volume>93</volume><issue>2</issue><spage>283</spage><epage>292</epage><pages>283-292</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Aqueous extracts of various lymphoid tissues, but not of non-lymphoid tissues, contain a species-non-specific but cell-specific inhibitor of the transformation and DNA synthesis of PHA-stimulated human lymphocytes which is apparently not cytotoxic and is reversible. This activity is found in similar molecular weight fractions from pure lymphocytes obtained in culture and hence appears to be endogenous to the lymphocyte itself. This specific and endogenous mitotic inhibitor does not appear to be a result of competitive lectin-binding, thymidine pool size dilution, phosphorylation, destruction of thymidine, or the direct immunosuppressive effects of thymidine upon the lymphocytes themselves. Rather, it appears to be a result of the effects of a protein contained in the crude ultrafiltrate from lymphoid tissues whose properties correspond to those originally described by Bullough & Laurence for a ‘chalone’. The chalone activity from thymus appears to be specific for T cells rather than B cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>125653</pmid><doi>10.1016/0014-4827(75)90452-8</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 1975-07, Vol.93 (2), p.283-292 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals B-Lymphocytes - metabolism Bone Marrow - metabolism Bone Marrow Cells Cattle Cell Line Cell Survival - drug effects DNA - biosynthesis Dose-Response Relationship, Drug Fibroblasts - metabolism Growth Inhibitors - isolation & purification Growth Inhibitors - pharmacology HeLa Cells - metabolism Humans Lectins Lung - analysis Lymph Nodes - analysis Lymphocyte Activation - drug effects Mice Muscles - analysis Species Specificity Spleen - analysis T-Lymphocytes - metabolism Thymidine - pharmacology |
| title | The specific and endogenous mitotic inhibitor of lymphocytes (chalone) |
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