Localization of tropomyosin in mouse embryo fibroblasts
Antiserum to chick skeletal muscle tropomyosin was used to localize tropomyosin in mouse embryo fibroblasts by the indirect fluorescein labeled antibody technique. Specific staining was observed in cytoplasmic fibers, which extended out into the cell processes. The staining pattern in these cells is...
Gespeichert in:
| Veröffentlicht in: | American journal of anatomy 1975-04, Vol.142 (4), p.519-525 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 525 |
|---|---|
| container_issue | 4 |
| container_start_page | 519 |
| container_title | American journal of anatomy |
| container_volume | 142 |
| creator | Jorgensen, A. O. Subrahmanyan, L. Kalnins, V. I. |
| description | Antiserum to chick skeletal muscle tropomyosin was used to localize tropomyosin in mouse embryo fibroblasts by the indirect fluorescein labeled antibody technique. Specific staining was observed in cytoplasmic fibers, which extended out into the cell processes. The staining pattern in these cells is similar to that previously described by others for actin. This observation suggests that in fibroblasts tropomyosin, like actin, is localized in fibers in the cytoplasm. |
| doi_str_mv | 10.1002/aja.1001420409 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82943606</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>82943606</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3389-67463df27b2162eb624a3f3730233e0bc4ad49c830402e73cba9e1047363829d3</originalsourceid><addsrcrecordid>eNqFkM1LAzEQxYNYtFavXrzsydvWSSabNMdSrB8UvOg5JLtZ2LLb1GSLrH-9qS3qTRiYGeY3j8cj5JrClAKwO7M2-4FyBhzUCRnTosAcpCpOyRgSkSsK4pxcxLhO64wpOCOjAiQTYyJXvjRt82n6xm8yX2d98FvfDT42myxV53fRZa6zYfBZ3djgbWtiHy_JqDZtdFfHPiFvy_vXxWO-enl4WsxXeYk4U7mQXGBVM2kZFcxZwbjBGiUCQ3RgS24qrsoZJuvMSSytUY4ClygwGa1wQm4Putvg33cu9rprYuna1mxcsqYTxFGASOD0AJbBxxhcrbeh6UwYNAW9z0mnnPRvTunh5qi8s52rfvDvYNJVHa4fTeuGf7T0_Hn-R_kLfGRyJA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82943606</pqid></control><display><type>article</type><title>Localization of tropomyosin in mouse embryo fibroblasts</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Jorgensen, A. O. ; Subrahmanyan, L. ; Kalnins, V. I.</creator><creatorcontrib>Jorgensen, A. O. ; Subrahmanyan, L. ; Kalnins, V. I.</creatorcontrib><description>Antiserum to chick skeletal muscle tropomyosin was used to localize tropomyosin in mouse embryo fibroblasts by the indirect fluorescein labeled antibody technique. Specific staining was observed in cytoplasmic fibers, which extended out into the cell processes. The staining pattern in these cells is similar to that previously described by others for actin. This observation suggests that in fibroblasts tropomyosin, like actin, is localized in fibers in the cytoplasm.</description><identifier>ISSN: 0002-9106</identifier><identifier>EISSN: 1553-0795</identifier><identifier>DOI: 10.1002/aja.1001420409</identifier><identifier>PMID: 50726</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Actins - metabolism ; Animals ; Chickens ; Cytoplasm - ultrastructure ; Electrophoresis, Polyacrylamide Gel ; Embryo, Mammalian ; Embryo, Nonmammalian ; Fibroblasts - analysis ; Fluorescent Antibody Technique ; Immune Sera ; Mice ; Muscles - physiology ; Rabbits ; Staining and Labeling ; Tropomyosin - analysis</subject><ispartof>American journal of anatomy, 1975-04, Vol.142 (4), p.519-525</ispartof><rights>Copyright © 1975 Wiley‐Liss, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3389-67463df27b2162eb624a3f3730233e0bc4ad49c830402e73cba9e1047363829d3</citedby><cites>FETCH-LOGICAL-c3389-67463df27b2162eb624a3f3730233e0bc4ad49c830402e73cba9e1047363829d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Faja.1001420409$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Faja.1001420409$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/50726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jorgensen, A. O.</creatorcontrib><creatorcontrib>Subrahmanyan, L.</creatorcontrib><creatorcontrib>Kalnins, V. I.</creatorcontrib><title>Localization of tropomyosin in mouse embryo fibroblasts</title><title>American journal of anatomy</title><addtitle>Am J Anat</addtitle><description>Antiserum to chick skeletal muscle tropomyosin was used to localize tropomyosin in mouse embryo fibroblasts by the indirect fluorescein labeled antibody technique. Specific staining was observed in cytoplasmic fibers, which extended out into the cell processes. The staining pattern in these cells is similar to that previously described by others for actin. This observation suggests that in fibroblasts tropomyosin, like actin, is localized in fibers in the cytoplasm.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Chickens</subject><subject>Cytoplasm - ultrastructure</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Embryo, Mammalian</subject><subject>Embryo, Nonmammalian</subject><subject>Fibroblasts - analysis</subject><subject>Fluorescent Antibody Technique</subject><subject>Immune Sera</subject><subject>Mice</subject><subject>Muscles - physiology</subject><subject>Rabbits</subject><subject>Staining and Labeling</subject><subject>Tropomyosin - analysis</subject><issn>0002-9106</issn><issn>1553-0795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LAzEQxYNYtFavXrzsydvWSSabNMdSrB8UvOg5JLtZ2LLb1GSLrH-9qS3qTRiYGeY3j8cj5JrClAKwO7M2-4FyBhzUCRnTosAcpCpOyRgSkSsK4pxcxLhO64wpOCOjAiQTYyJXvjRt82n6xm8yX2d98FvfDT42myxV53fRZa6zYfBZ3djgbWtiHy_JqDZtdFfHPiFvy_vXxWO-enl4WsxXeYk4U7mQXGBVM2kZFcxZwbjBGiUCQ3RgS24qrsoZJuvMSSytUY4ClygwGa1wQm4Putvg33cu9rprYuna1mxcsqYTxFGASOD0AJbBxxhcrbeh6UwYNAW9z0mnnPRvTunh5qi8s52rfvDvYNJVHa4fTeuGf7T0_Hn-R_kLfGRyJA</recordid><startdate>197504</startdate><enddate>197504</enddate><creator>Jorgensen, A. O.</creator><creator>Subrahmanyan, L.</creator><creator>Kalnins, V. I.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197504</creationdate><title>Localization of tropomyosin in mouse embryo fibroblasts</title><author>Jorgensen, A. O. ; Subrahmanyan, L. ; Kalnins, V. I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3389-67463df27b2162eb624a3f3730233e0bc4ad49c830402e73cba9e1047363829d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Chickens</topic><topic>Cytoplasm - ultrastructure</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Embryo, Mammalian</topic><topic>Embryo, Nonmammalian</topic><topic>Fibroblasts - analysis</topic><topic>Fluorescent Antibody Technique</topic><topic>Immune Sera</topic><topic>Mice</topic><topic>Muscles - physiology</topic><topic>Rabbits</topic><topic>Staining and Labeling</topic><topic>Tropomyosin - analysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Jorgensen, A. O.</creatorcontrib><creatorcontrib>Subrahmanyan, L.</creatorcontrib><creatorcontrib>Kalnins, V. I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of anatomy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jorgensen, A. O.</au><au>Subrahmanyan, L.</au><au>Kalnins, V. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of tropomyosin in mouse embryo fibroblasts</atitle><jtitle>American journal of anatomy</jtitle><addtitle>Am J Anat</addtitle><date>1975-04</date><risdate>1975</risdate><volume>142</volume><issue>4</issue><spage>519</spage><epage>525</epage><pages>519-525</pages><issn>0002-9106</issn><eissn>1553-0795</eissn><abstract>Antiserum to chick skeletal muscle tropomyosin was used to localize tropomyosin in mouse embryo fibroblasts by the indirect fluorescein labeled antibody technique. Specific staining was observed in cytoplasmic fibers, which extended out into the cell processes. The staining pattern in these cells is similar to that previously described by others for actin. This observation suggests that in fibroblasts tropomyosin, like actin, is localized in fibers in the cytoplasm.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>50726</pmid><doi>10.1002/aja.1001420409</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9106 |
| ispartof | American journal of anatomy, 1975-04, Vol.142 (4), p.519-525 |
| issn | 0002-9106 1553-0795 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82943606 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Actins - metabolism Animals Chickens Cytoplasm - ultrastructure Electrophoresis, Polyacrylamide Gel Embryo, Mammalian Embryo, Nonmammalian Fibroblasts - analysis Fluorescent Antibody Technique Immune Sera Mice Muscles - physiology Rabbits Staining and Labeling Tropomyosin - analysis |
| title | Localization of tropomyosin in mouse embryo fibroblasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T15%3A28%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Localization%20of%20tropomyosin%20in%20mouse%20embryo%20fibroblasts&rft.jtitle=American%20journal%20of%20anatomy&rft.au=Jorgensen,%20A.%20O.&rft.date=1975-04&rft.volume=142&rft.issue=4&rft.spage=519&rft.epage=525&rft.pages=519-525&rft.issn=0002-9106&rft.eissn=1553-0795&rft_id=info:doi/10.1002/aja.1001420409&rft_dat=%3Cproquest_cross%3E82943606%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82943606&rft_id=info:pmid/50726&rfr_iscdi=true |