Studies on energy-linked reactions: isolation and properties of mitochondrial venturicidin-resistant mutants of Saccharomyces cerevisiae
Venturicidin is a specific inhibitor of aerobic growth of yeast and has no effect on fermentative growth, a result which is consistent with its known mode of action on mitochondrial oxidative phosphorylation. Venturicidin‐resistant mutants of Saccharomyces cerevisiae have been isolated and form two...
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| Veröffentlicht in: | European journal of biochemistry 1975-01, Vol.51 (2), p.393-402 |
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| container_title | European journal of biochemistry |
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| creator | Griffiths, D.E Houghton, R.L Lanchashire, W.E Meadows, P.A |
| description | Venturicidin is a specific inhibitor of aerobic growth of yeast and has no effect on fermentative growth, a result which is consistent with its known mode of action on mitochondrial oxidative phosphorylation.
Venturicidin‐resistant mutants of Saccharomyces cerevisiae have been isolated and form two general classes: class 1, nuclear mutants which are resistant to a variety of mitochondrial inhibitors and uncouplers, and class 2, mitochondrial mutants of phenotype VENR OLYR and VENR TETRin vivo. VENR OLYR mutants show a high degree of resistance to venturicidin and oligomycin at the whole cell and mitochondrial ATPase level but, in contrast, no resistance at the mitochondrial level is observed with VENR TETR mutants.
Venturicidin resistance/sensitivity can be correlated with two binding sites on mitochondrial ATPase, one of which is common to the oligomycin binding site and the other is common to the triethyl tin binding site. Biochemical genetic studies indicate that two mitochondrial genes specify venturicidin resistance/sensitivity and that the mitochondrial gene products are components of the mitochondrial ATPase complex. |
| doi_str_mv | 10.1111/j.1432-1033.1975.tb03939.x |
| format | Article |
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Venturicidin‐resistant mutants of Saccharomyces cerevisiae have been isolated and form two general classes: class 1, nuclear mutants which are resistant to a variety of mitochondrial inhibitors and uncouplers, and class 2, mitochondrial mutants of phenotype VENR OLYR and VENR TETRin vivo. VENR OLYR mutants show a high degree of resistance to venturicidin and oligomycin at the whole cell and mitochondrial ATPase level but, in contrast, no resistance at the mitochondrial level is observed with VENR TETR mutants.
Venturicidin resistance/sensitivity can be correlated with two binding sites on mitochondrial ATPase, one of which is common to the oligomycin binding site and the other is common to the triethyl tin binding site. Biochemical genetic studies indicate that two mitochondrial genes specify venturicidin resistance/sensitivity and that the mitochondrial gene products are components of the mitochondrial ATPase complex.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1975.tb03939.x</identifier><identifier>PMID: 238835</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenosine Triphosphatases - metabolism ; Aerobiosis ; Antifungal Agents - pharmacology ; Cell Division - drug effects ; Drug Resistance, Microbial ; Ethanol - metabolism ; Genes ; Glucose - metabolism ; Hydrogen-Ion Concentration ; Kinetics ; Lactones - pharmacology ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mutation ; Oligomycins - pharmacology ; Oxidative Phosphorylation - drug effects ; Phenotype ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - metabolism ; Triethyltin Compounds - pharmacology ; yeasts</subject><ispartof>European journal of biochemistry, 1975-01, Vol.51 (2), p.393-402</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4453-e3049b0a9337ff81dca80b8b58dc894def5c4d183e4c1fbdf9514b8d8269469a3</citedby><cites>FETCH-LOGICAL-c4453-e3049b0a9337ff81dca80b8b58dc894def5c4d183e4c1fbdf9514b8d8269469a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/238835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Griffiths, D.E</creatorcontrib><creatorcontrib>Houghton, R.L</creatorcontrib><creatorcontrib>Lanchashire, W.E</creatorcontrib><creatorcontrib>Meadows, P.A</creatorcontrib><title>Studies on energy-linked reactions: isolation and properties of mitochondrial venturicidin-resistant mutants of Saccharomyces cerevisiae</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>Venturicidin is a specific inhibitor of aerobic growth of yeast and has no effect on fermentative growth, a result which is consistent with its known mode of action on mitochondrial oxidative phosphorylation.
Venturicidin‐resistant mutants of Saccharomyces cerevisiae have been isolated and form two general classes: class 1, nuclear mutants which are resistant to a variety of mitochondrial inhibitors and uncouplers, and class 2, mitochondrial mutants of phenotype VENR OLYR and VENR TETRin vivo. VENR OLYR mutants show a high degree of resistance to venturicidin and oligomycin at the whole cell and mitochondrial ATPase level but, in contrast, no resistance at the mitochondrial level is observed with VENR TETR mutants.
Venturicidin resistance/sensitivity can be correlated with two binding sites on mitochondrial ATPase, one of which is common to the oligomycin binding site and the other is common to the triethyl tin binding site. Biochemical genetic studies indicate that two mitochondrial genes specify venturicidin resistance/sensitivity and that the mitochondrial gene products are components of the mitochondrial ATPase complex.</description><subject>Adenosine Triphosphatases - metabolism</subject><subject>Aerobiosis</subject><subject>Antifungal Agents - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Drug Resistance, Microbial</subject><subject>Ethanol - metabolism</subject><subject>Genes</subject><subject>Glucose - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Lactones - pharmacology</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Mutation</subject><subject>Oligomycins - pharmacology</subject><subject>Oxidative Phosphorylation - drug effects</subject><subject>Phenotype</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Triethyltin Compounds - pharmacology</subject><subject>yeasts</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUctu1DAUtRCvofAHSEQs2CXYsZ3Y3SCoWkCqxGLo2nLsm9ZDYg92Ujp_0M-u04y6x5t7pfO48jkIfSS4Ivl93lWE0bokmNKKyJZXU4eppLK6e4Y2T9BztMGYsLKWvHmN3qS0wxg3smlfoZc1FYLyDbrfTrN1kIrgC_AQrw_l4PwfsEUEbSYXfDotXAqDXvZCe1vsY9hDnB5FfTG6KZib4G10eihuwU9zdMZZ58sIyaVJ-6kY52U88rfamBsdw3gw2cBAhFuXnIa36EWvhwTvjvMEXV2c_z77UV7--v7z7OtlaRjjtASKmeywlpS2fS-INVrgTnRcWCMks9BzwywRFJghfWd7yQnrhBV1I1kjNT1Bn1bf_I2_M6RJjS4ZGAbtIcxJiVpS3sg2E09XookhpQi92kc36nhQBKulBbVTS9RqiVotLahjC-oui98fr8zdCPZJusae4S8r_M8NcPgPY3Vx_m2b1-zwYXXodVD6OrqkrrY1JhTXLeGZSx8A9ualZw</recordid><startdate>19750101</startdate><enddate>19750101</enddate><creator>Griffiths, D.E</creator><creator>Houghton, R.L</creator><creator>Lanchashire, W.E</creator><creator>Meadows, P.A</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750101</creationdate><title>Studies on energy-linked reactions: isolation and properties of mitochondrial venturicidin-resistant mutants of Saccharomyces cerevisiae</title><author>Griffiths, D.E ; Houghton, R.L ; Lanchashire, W.E ; Meadows, P.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4453-e3049b0a9337ff81dca80b8b58dc894def5c4d183e4c1fbdf9514b8d8269469a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Adenosine Triphosphatases - metabolism</topic><topic>Aerobiosis</topic><topic>Antifungal Agents - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Drug Resistance, Microbial</topic><topic>Ethanol - metabolism</topic><topic>Genes</topic><topic>Glucose - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Lactones - pharmacology</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Mutation</topic><topic>Oligomycins - pharmacology</topic><topic>Oxidative Phosphorylation - drug effects</topic><topic>Phenotype</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Triethyltin Compounds - pharmacology</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Griffiths, D.E</creatorcontrib><creatorcontrib>Houghton, R.L</creatorcontrib><creatorcontrib>Lanchashire, W.E</creatorcontrib><creatorcontrib>Meadows, P.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Griffiths, D.E</au><au>Houghton, R.L</au><au>Lanchashire, W.E</au><au>Meadows, P.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on energy-linked reactions: isolation and properties of mitochondrial venturicidin-resistant mutants of Saccharomyces cerevisiae</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1975-01-01</date><risdate>1975</risdate><volume>51</volume><issue>2</issue><spage>393</spage><epage>402</epage><pages>393-402</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><abstract>Venturicidin is a specific inhibitor of aerobic growth of yeast and has no effect on fermentative growth, a result which is consistent with its known mode of action on mitochondrial oxidative phosphorylation.
Venturicidin‐resistant mutants of Saccharomyces cerevisiae have been isolated and form two general classes: class 1, nuclear mutants which are resistant to a variety of mitochondrial inhibitors and uncouplers, and class 2, mitochondrial mutants of phenotype VENR OLYR and VENR TETRin vivo. VENR OLYR mutants show a high degree of resistance to venturicidin and oligomycin at the whole cell and mitochondrial ATPase level but, in contrast, no resistance at the mitochondrial level is observed with VENR TETR mutants.
Venturicidin resistance/sensitivity can be correlated with two binding sites on mitochondrial ATPase, one of which is common to the oligomycin binding site and the other is common to the triethyl tin binding site. Biochemical genetic studies indicate that two mitochondrial genes specify venturicidin resistance/sensitivity and that the mitochondrial gene products are components of the mitochondrial ATPase complex.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>238835</pmid><doi>10.1111/j.1432-1033.1975.tb03939.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | European journal of biochemistry, 1975-01, Vol.51 (2), p.393-402 |
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| language | eng |
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| subjects | Adenosine Triphosphatases - metabolism Aerobiosis Antifungal Agents - pharmacology Cell Division - drug effects Drug Resistance, Microbial Ethanol - metabolism Genes Glucose - metabolism Hydrogen-Ion Concentration Kinetics Lactones - pharmacology Mitochondria - drug effects Mitochondria - metabolism Mutation Oligomycins - pharmacology Oxidative Phosphorylation - drug effects Phenotype Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - metabolism Triethyltin Compounds - pharmacology yeasts |
| title | Studies on energy-linked reactions: isolation and properties of mitochondrial venturicidin-resistant mutants of Saccharomyces cerevisiae |
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