Histochemical Studies of Succinic Dehydrogenase in Trypanosoma cruzi and Leishmania leproide
Summary Succinic dehydrogenase has been demonstrated histochemically in T. cruzi and L. leproide - leptomonad form. The protozoa were stained for the enzyme with nitro blue tetrazolium in presence of brilliant cresyl blue. Optimal requirements for the reaction have been described. Enzyme activity is...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1962-07, Vol.110 (3), p.517-521 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 110 |
| creator | Chakravarty, N. Sánchez, Marcolina Ercoli, N. |
| description | Summary
Succinic dehydrogenase has been demonstrated histochemically in T.
cruzi and L. leproide - leptomonad form. The protozoa were stained for the enzyme with nitro blue tetrazolium in presence of brilliant cresyl blue. Optimal requirements for the reaction have been described. Enzyme activity is indicated by blue deposits of diformazan in granular form in the cytoplasm. The reaction is weak in absence of calcium and bicarbonate and is inhibited by malonate. Spirotrypan had some inhibitory effect on the tetrazolium reaction in T. cruzi both in vitro and in vivo. |
| doi_str_mv | 10.3181/00379727-110-27567 |
| format | Article |
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Succinic dehydrogenase has been demonstrated histochemically in T.
cruzi and L. leproide - leptomonad form. The protozoa were stained for the enzyme with nitro blue tetrazolium in presence of brilliant cresyl blue. Optimal requirements for the reaction have been described. Enzyme activity is indicated by blue deposits of diformazan in granular form in the cytoplasm. The reaction is weak in absence of calcium and bicarbonate and is inhibited by malonate. Spirotrypan had some inhibitory effect on the tetrazolium reaction in T. cruzi both in vitro and in vivo.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-110-27567</identifier><identifier>PMID: 13877910</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Electron Transport Complex II ; Histological Techniques ; Leishmania - anatomy & histology ; Old Medline ; Succinate Dehydrogenase - chemistry ; Trypanosoma - chemistry ; Trypanosoma cruzi</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1962-07, Vol.110 (3), p.517-521</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-9a908c0062888d7b581ebb788d4e712713ded7d8aef0c789695e95bd570277683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13877910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chakravarty, N.</creatorcontrib><creatorcontrib>Sánchez, Marcolina</creatorcontrib><creatorcontrib>Ercoli, N.</creatorcontrib><title>Histochemical Studies of Succinic Dehydrogenase in Trypanosoma cruzi and Leishmania leproide</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Succinic dehydrogenase has been demonstrated histochemically in T.
cruzi and L. leproide - leptomonad form. The protozoa were stained for the enzyme with nitro blue tetrazolium in presence of brilliant cresyl blue. Optimal requirements for the reaction have been described. Enzyme activity is indicated by blue deposits of diformazan in granular form in the cytoplasm. The reaction is weak in absence of calcium and bicarbonate and is inhibited by malonate. Spirotrypan had some inhibitory effect on the tetrazolium reaction in T. cruzi both in vitro and in vivo.</description><subject>Electron Transport Complex II</subject><subject>Histological Techniques</subject><subject>Leishmania - anatomy & histology</subject><subject>Old Medline</subject><subject>Succinate Dehydrogenase - chemistry</subject><subject>Trypanosoma - chemistry</subject><subject>Trypanosoma cruzi</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1962</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EoqXwBxiQJ7aAHZOcPSK-ilSJgbIhWY59pa6SuNjJUH49KS1iY7obnvc93UPIOWdXgkt-zZgABTlknLMsh6KEAzLmhSgyUSp1SMZbINsSI3KS0ooxXkBeHpMRFxJAcTYm71OfumCX2Hhravra9c5jomFBX3trfestvcflxsXwga1JSH1L53GzNm1IoTHUxv7LU9M6OkOflo1pvaE1rmPwDk_J0cLUCc_2c0LeHh_md9Ns9vL0fHc7y6wQqsuUUUxaxspcSumgKiTHqoJhv0HgOXDh0IGTBhfMglSlKlAVlSuA5QClFBNyuesdzn72mDrd-GSxrk2LoU9a5krcSCUGMN-BNoaUIi70OvrGxI3mTG-d6l-nenCqf5wOoYt9e1816P4ie4kDcL0DkvlAvQp9bIdv_6v8Bu6Rf9U</recordid><startdate>196207</startdate><enddate>196207</enddate><creator>Chakravarty, N.</creator><creator>Sánchez, Marcolina</creator><creator>Ercoli, N.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196207</creationdate><title>Histochemical Studies of Succinic Dehydrogenase in Trypanosoma cruzi and Leishmania leproide</title><author>Chakravarty, N. ; Sánchez, Marcolina ; Ercoli, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-9a908c0062888d7b581ebb788d4e712713ded7d8aef0c789695e95bd570277683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1962</creationdate><topic>Electron Transport Complex II</topic><topic>Histological Techniques</topic><topic>Leishmania - anatomy & histology</topic><topic>Old Medline</topic><topic>Succinate Dehydrogenase - chemistry</topic><topic>Trypanosoma - chemistry</topic><topic>Trypanosoma cruzi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chakravarty, N.</creatorcontrib><creatorcontrib>Sánchez, Marcolina</creatorcontrib><creatorcontrib>Ercoli, N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chakravarty, N.</au><au>Sánchez, Marcolina</au><au>Ercoli, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histochemical Studies of Succinic Dehydrogenase in Trypanosoma cruzi and Leishmania leproide</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1962-07</date><risdate>1962</risdate><volume>110</volume><issue>3</issue><spage>517</spage><epage>521</epage><pages>517-521</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Succinic dehydrogenase has been demonstrated histochemically in T.
cruzi and L. leproide - leptomonad form. The protozoa were stained for the enzyme with nitro blue tetrazolium in presence of brilliant cresyl blue. Optimal requirements for the reaction have been described. Enzyme activity is indicated by blue deposits of diformazan in granular form in the cytoplasm. The reaction is weak in absence of calcium and bicarbonate and is inhibited by malonate. Spirotrypan had some inhibitory effect on the tetrazolium reaction in T. cruzi both in vitro and in vivo.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>13877910</pmid><doi>10.3181/00379727-110-27567</doi><tpages>5</tpages></addata></record> |
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| ispartof | Experimental biology and medicine (Maywood, N.J.), 1962-07, Vol.110 (3), p.517-521 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Electron Transport Complex II Histological Techniques Leishmania - anatomy & histology Old Medline Succinate Dehydrogenase - chemistry Trypanosoma - chemistry Trypanosoma cruzi |
| title | Histochemical Studies of Succinic Dehydrogenase in Trypanosoma cruzi and Leishmania leproide |
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