Studies on the de novo biosynthesis of NAD in Escherichia coli. The separation of the nadB gene product from the nadA gene product and its purification

Quinolinic acid (pyridine 2,3-dicarboxylic acid) which is an immediate precursor of the pyridine nucleotides, is synthesised from L-asparate and dihydroxyacetone phosphate in Escherichia coli. Extracts from certain nadB mutants complement the extracts prepared from all nadA mutants for the enzymic s...

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Veröffentlicht in:	European journal of biochemistry 1975-05, Vol.54 (1), p.239-245
Hauptverfasser:	Griffith, G R, Chandler, J L, Gholson, R K
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspartic Acid Chromatography Chromatography, Gel Chromatography, Ion Exchange Escherichia coli - enzymology Escherichia coli - metabolism Genetic Complementation Test Genetics, Microbial Hydroxyapatites Macromolecular Substances Multienzyme Complexes - antagonists & inhibitors Multienzyme Complexes - isolation & purification Mutation NAD - biosynthesis NADP Organophosphorus Compounds Protein Biosynthesis Trioses
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