Lysophospholipids in Pig Pancreatic Zymogen Granules in Relation to Exocytosis
A hypothesis to explain the stimulatory role of cyclic AMP (adenosine 3′:5′‐monophosphate) in pancreatic enzyme secretion has been tested. In this hypothesis cyclic AMP would activate a phospholipase activity, which would lead to a locally increased lysophospholipid formation, resulting in a fusion...
Gespeichert in:
| Veröffentlicht in: | European journal of biochemistry 1975-05, Vol.54 (1), p.259-265 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 265 |
|---|---|
| container_issue | 1 |
| container_start_page | 259 |
| container_title | European journal of biochemistry |
| container_volume | 54 |
| creator | RUTTEN, Willem J. PONT, Jan Joep H. H. M. BONTING, Sjoerd L. DAEMEN, Frans J. M. |
| description | A hypothesis to explain the stimulatory role of cyclic AMP (adenosine 3′:5′‐monophosphate) in pancreatic enzyme secretion has been tested. In this hypothesis cyclic AMP would activate a phospholipase activity, which would lead to a locally increased lysophospholipid formation, resulting in a fusion between the zymogen granule membrane and the apical plasma membrane.
Cyclic AMP added to isolated pig pancreatic zymogen granules leads to an increased lysis of these granules, but the slowness of this effect makes its physiological significance dubious. In pancreatic homogenates or zymogen granules no stimulating effect of cyclic AMP on lipase or phospholipase activity could be demonstrated.
Isolated zymogen granules have a high lysophospholipid content (27% of total phospholipids), consisting of the 1‐acyl and 2‐acyl forms of lysophosphatidylcholine and lysophosphatidylethanolamine. Experiments with radioactive phosphatidylcholine indicate that the lysophospholipids are due to the action of endogenous (phospho)lipases during the isolation procedure.
It is concluded that these experiments do not lend support to the above hypothesis for the mechanism of action of cyclic AMP in pancreatic enzyme secretion. |
| doi_str_mv | 10.1111/j.1432-1033.1975.tb04136.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82925406</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>82925406</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4219-e4b8596d3a4b05e55fe794cd6382c5b3dfca9a29508c4935f586072af4a785f63</originalsourceid><addsrcrecordid>eNqVkMtOwzAQRS3EqxT-gEXEgl2CHT8Ss0FQtQWpgorHho3lOE5xlcQhTkTz9yS0gjUjjWZx750ZHQAuEAxQX1frABEc-ghiHCAe0aBJIEGYBZs9MPqV9sEIQkT8kFN2DE6cW0MIGWfREThELIZRPAKPi87Z6sO6vnNTmdR5pvSWZuUtZalqLRujvPeusCtdevNalm2ufyzPOu81W3qN9aYbq7rGOuNOwUEmc6fPdnMM3mbT18m9v3iaP0xuF74iIeK-JklMOUuxJAmkmtJMR5yolOE4VDTBaaYkl_3fMFaEY5rRmMEolBmRUUwzhsfgcru3qu1nq10jCuOUznNZats6EYc8pAQOxuutUdXWuVpnoqpNIetOICgGlmItBmBiACYGlmLHUmz68PnuSpsUOv2L_sDr5Zut_GVy3f1jsZhN715CyvE3iASEfg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82925406</pqid></control><display><type>article</type><title>Lysophospholipids in Pig Pancreatic Zymogen Granules in Relation to Exocytosis</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>RUTTEN, Willem J. ; PONT, Jan Joep H. H. M. ; BONTING, Sjoerd L. ; DAEMEN, Frans J. M.</creator><creatorcontrib>RUTTEN, Willem J. ; PONT, Jan Joep H. H. M. ; BONTING, Sjoerd L. ; DAEMEN, Frans J. M.</creatorcontrib><description>A hypothesis to explain the stimulatory role of cyclic AMP (adenosine 3′:5′‐monophosphate) in pancreatic enzyme secretion has been tested. In this hypothesis cyclic AMP would activate a phospholipase activity, which would lead to a locally increased lysophospholipid formation, resulting in a fusion between the zymogen granule membrane and the apical plasma membrane.
Cyclic AMP added to isolated pig pancreatic zymogen granules leads to an increased lysis of these granules, but the slowness of this effect makes its physiological significance dubious. In pancreatic homogenates or zymogen granules no stimulating effect of cyclic AMP on lipase or phospholipase activity could be demonstrated.
Isolated zymogen granules have a high lysophospholipid content (27% of total phospholipids), consisting of the 1‐acyl and 2‐acyl forms of lysophosphatidylcholine and lysophosphatidylethanolamine. Experiments with radioactive phosphatidylcholine indicate that the lysophospholipids are due to the action of endogenous (phospho)lipases during the isolation procedure.
It is concluded that these experiments do not lend support to the above hypothesis for the mechanism of action of cyclic AMP in pancreatic enzyme secretion.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1975.tb04136.x</identifier><identifier>PMID: 168078</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Cell Fractionation ; Cells ; Cyclic AMP - pharmacology ; Cytoplasmic Granules - analysis ; Cytoplasmic Granules - drug effects ; Cytoplasmic Granules - metabolism ; Exocytosis ; Lipase ; Pancreas - drug effects ; Pancreas - enzymology ; Pancreas - metabolism ; Pancreas - ultrastructure ; Phosphatidylcholines ; Phospholipases ; Phospholipids - analysis ; Swine</subject><ispartof>European journal of biochemistry, 1975-05, Vol.54 (1), p.259-265</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4219-e4b8596d3a4b05e55fe794cd6382c5b3dfca9a29508c4935f586072af4a785f63</citedby><cites>FETCH-LOGICAL-c4219-e4b8596d3a4b05e55fe794cd6382c5b3dfca9a29508c4935f586072af4a785f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/168078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RUTTEN, Willem J.</creatorcontrib><creatorcontrib>PONT, Jan Joep H. H. M.</creatorcontrib><creatorcontrib>BONTING, Sjoerd L.</creatorcontrib><creatorcontrib>DAEMEN, Frans J. M.</creatorcontrib><title>Lysophospholipids in Pig Pancreatic Zymogen Granules in Relation to Exocytosis</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>A hypothesis to explain the stimulatory role of cyclic AMP (adenosine 3′:5′‐monophosphate) in pancreatic enzyme secretion has been tested. In this hypothesis cyclic AMP would activate a phospholipase activity, which would lead to a locally increased lysophospholipid formation, resulting in a fusion between the zymogen granule membrane and the apical plasma membrane.
Cyclic AMP added to isolated pig pancreatic zymogen granules leads to an increased lysis of these granules, but the slowness of this effect makes its physiological significance dubious. In pancreatic homogenates or zymogen granules no stimulating effect of cyclic AMP on lipase or phospholipase activity could be demonstrated.
Isolated zymogen granules have a high lysophospholipid content (27% of total phospholipids), consisting of the 1‐acyl and 2‐acyl forms of lysophosphatidylcholine and lysophosphatidylethanolamine. Experiments with radioactive phosphatidylcholine indicate that the lysophospholipids are due to the action of endogenous (phospho)lipases during the isolation procedure.
It is concluded that these experiments do not lend support to the above hypothesis for the mechanism of action of cyclic AMP in pancreatic enzyme secretion.</description><subject>Animals</subject><subject>Cell Fractionation</subject><subject>Cells</subject><subject>Cyclic AMP - pharmacology</subject><subject>Cytoplasmic Granules - analysis</subject><subject>Cytoplasmic Granules - drug effects</subject><subject>Cytoplasmic Granules - metabolism</subject><subject>Exocytosis</subject><subject>Lipase</subject><subject>Pancreas - drug effects</subject><subject>Pancreas - enzymology</subject><subject>Pancreas - metabolism</subject><subject>Pancreas - ultrastructure</subject><subject>Phosphatidylcholines</subject><subject>Phospholipases</subject><subject>Phospholipids - analysis</subject><subject>Swine</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMtOwzAQRS3EqxT-gEXEgl2CHT8Ss0FQtQWpgorHho3lOE5xlcQhTkTz9yS0gjUjjWZx750ZHQAuEAxQX1frABEc-ghiHCAe0aBJIEGYBZs9MPqV9sEIQkT8kFN2DE6cW0MIGWfREThELIZRPAKPi87Z6sO6vnNTmdR5pvSWZuUtZalqLRujvPeusCtdevNalm2ufyzPOu81W3qN9aYbq7rGOuNOwUEmc6fPdnMM3mbT18m9v3iaP0xuF74iIeK-JklMOUuxJAmkmtJMR5yolOE4VDTBaaYkl_3fMFaEY5rRmMEolBmRUUwzhsfgcru3qu1nq10jCuOUznNZats6EYc8pAQOxuutUdXWuVpnoqpNIetOICgGlmItBmBiACYGlmLHUmz68PnuSpsUOv2L_sDr5Zut_GVy3f1jsZhN715CyvE3iASEfg</recordid><startdate>197505</startdate><enddate>197505</enddate><creator>RUTTEN, Willem J.</creator><creator>PONT, Jan Joep H. H. M.</creator><creator>BONTING, Sjoerd L.</creator><creator>DAEMEN, Frans J. M.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197505</creationdate><title>Lysophospholipids in Pig Pancreatic Zymogen Granules in Relation to Exocytosis</title><author>RUTTEN, Willem J. ; PONT, Jan Joep H. H. M. ; BONTING, Sjoerd L. ; DAEMEN, Frans J. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4219-e4b8596d3a4b05e55fe794cd6382c5b3dfca9a29508c4935f586072af4a785f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Cell Fractionation</topic><topic>Cells</topic><topic>Cyclic AMP - pharmacology</topic><topic>Cytoplasmic Granules - analysis</topic><topic>Cytoplasmic Granules - drug effects</topic><topic>Cytoplasmic Granules - metabolism</topic><topic>Exocytosis</topic><topic>Lipase</topic><topic>Pancreas - drug effects</topic><topic>Pancreas - enzymology</topic><topic>Pancreas - metabolism</topic><topic>Pancreas - ultrastructure</topic><topic>Phosphatidylcholines</topic><topic>Phospholipases</topic><topic>Phospholipids - analysis</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RUTTEN, Willem J.</creatorcontrib><creatorcontrib>PONT, Jan Joep H. H. M.</creatorcontrib><creatorcontrib>BONTING, Sjoerd L.</creatorcontrib><creatorcontrib>DAEMEN, Frans J. M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RUTTEN, Willem J.</au><au>PONT, Jan Joep H. H. M.</au><au>BONTING, Sjoerd L.</au><au>DAEMEN, Frans J. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lysophospholipids in Pig Pancreatic Zymogen Granules in Relation to Exocytosis</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1975-05</date><risdate>1975</risdate><volume>54</volume><issue>1</issue><spage>259</spage><epage>265</epage><pages>259-265</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><abstract>A hypothesis to explain the stimulatory role of cyclic AMP (adenosine 3′:5′‐monophosphate) in pancreatic enzyme secretion has been tested. In this hypothesis cyclic AMP would activate a phospholipase activity, which would lead to a locally increased lysophospholipid formation, resulting in a fusion between the zymogen granule membrane and the apical plasma membrane.
Cyclic AMP added to isolated pig pancreatic zymogen granules leads to an increased lysis of these granules, but the slowness of this effect makes its physiological significance dubious. In pancreatic homogenates or zymogen granules no stimulating effect of cyclic AMP on lipase or phospholipase activity could be demonstrated.
Isolated zymogen granules have a high lysophospholipid content (27% of total phospholipids), consisting of the 1‐acyl and 2‐acyl forms of lysophosphatidylcholine and lysophosphatidylethanolamine. Experiments with radioactive phosphatidylcholine indicate that the lysophospholipids are due to the action of endogenous (phospho)lipases during the isolation procedure.
It is concluded that these experiments do not lend support to the above hypothesis for the mechanism of action of cyclic AMP in pancreatic enzyme secretion.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>168078</pmid><doi>10.1111/j.1432-1033.1975.tb04136.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2956 |
| ispartof | European journal of biochemistry, 1975-05, Vol.54 (1), p.259-265 |
| issn | 0014-2956 1432-1033 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82925406 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Fractionation Cells Cyclic AMP - pharmacology Cytoplasmic Granules - analysis Cytoplasmic Granules - drug effects Cytoplasmic Granules - metabolism Exocytosis Lipase Pancreas - drug effects Pancreas - enzymology Pancreas - metabolism Pancreas - ultrastructure Phosphatidylcholines Phospholipases Phospholipids - analysis Swine |
| title | Lysophospholipids in Pig Pancreatic Zymogen Granules in Relation to Exocytosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T04%3A52%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lysophospholipids%20in%20Pig%20Pancreatic%20Zymogen%20Granules%20in%20Relation%20to%20Exocytosis&rft.jtitle=European%20journal%20of%20biochemistry&rft.au=RUTTEN,%20Willem%20J.&rft.date=1975-05&rft.volume=54&rft.issue=1&rft.spage=259&rft.epage=265&rft.pages=259-265&rft.issn=0014-2956&rft.eissn=1432-1033&rft_id=info:doi/10.1111/j.1432-1033.1975.tb04136.x&rft_dat=%3Cproquest_cross%3E82925406%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82925406&rft_id=info:pmid/168078&rfr_iscdi=true |