Failure to obtain transfection with XC mitochondrial DNA
The transfecting activity of DNA obtained from mitochondria of XC virogenic rat cells transformed with the Prague strain of RSV was tested. Both mitochondrial DNA isolated with EthBr, containing circular molecules, and total mitochondrial DNA were inefficient in transfecting sensitive chicken fibrob...
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| Veröffentlicht in: | European journal of cancer 1975-04, Vol.11 (4), p.247,IN5-250,IN5 |
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| container_end_page | 250,IN5 |
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| container_issue | 4 |
| container_start_page | 247,IN5 |
| container_title | European journal of cancer |
| container_volume | 11 |
| creator | Svoboda, Jan Hložánek, Ivo Korb, Jan Mach, Otakar |
| description | The transfecting activity of DNA obtained from mitochondria of XC virogenic rat cells transformed with the Prague strain of RSV was tested. Both mitochondrial DNA isolated with EthBr, containing circular molecules, and total mitochondrial DNA were inefficient in transfecting sensitive chicken fibroblasts in spite of the fact that doses of mitochondrial DNA up to two orders higher than the minimum efficient dose of total XC DNA were used. These findings support the view that a DNA transcript of the RSV genome is not integrated in mitochondrial DNA. |
| doi_str_mv | 10.1016/0014-2964(75)90005-5 |
| format | Article |
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Both mitochondrial DNA isolated with EthBr, containing circular molecules, and total mitochondrial DNA were inefficient in transfecting sensitive chicken fibroblasts in spite of the fact that doses of mitochondrial DNA up to two orders higher than the minimum efficient dose of total XC DNA were used. These findings support the view that a DNA transcript of the RSV genome is not integrated in mitochondrial DNA.</description><identifier>ISSN: 0014-2964</identifier><identifier>DOI: 10.1016/0014-2964(75)90005-5</identifier><identifier>PMID: 166846</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Avian Sarcoma Viruses ; Cell Line ; Cell Transformation, Neoplastic ; Chick Embryo ; Chickens ; DNA, Circular - isolation & purification ; DNA, Mitochondrial - isolation & purification ; DNA, Neoplasm ; DNA, Viral ; Ethidium ; Rats ; Sarcoma, Experimental - microbiology ; Sarcoma, Experimental - transmission ; Transformation, Genetic</subject><ispartof>European journal of cancer, 1975-04, Vol.11 (4), p.247,IN5-250,IN5</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-f837919c27e41a58290e80932506fdd876dd06a19684410c6290bb1328c2db393</citedby><cites>FETCH-LOGICAL-c356t-f837919c27e41a58290e80932506fdd876dd06a19684410c6290bb1328c2db393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/166846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Svoboda, Jan</creatorcontrib><creatorcontrib>Hložánek, Ivo</creatorcontrib><creatorcontrib>Korb, Jan</creatorcontrib><creatorcontrib>Mach, Otakar</creatorcontrib><title>Failure to obtain transfection with XC mitochondrial DNA</title><title>European journal of cancer</title><addtitle>Eur J Cancer</addtitle><description>The transfecting activity of DNA obtained from mitochondria of XC virogenic rat cells transformed with the Prague strain of RSV was tested. Both mitochondrial DNA isolated with EthBr, containing circular molecules, and total mitochondrial DNA were inefficient in transfecting sensitive chicken fibroblasts in spite of the fact that doses of mitochondrial DNA up to two orders higher than the minimum efficient dose of total XC DNA were used. These findings support the view that a DNA transcript of the RSV genome is not integrated in mitochondrial DNA.</description><subject>Animals</subject><subject>Avian Sarcoma Viruses</subject><subject>Cell Line</subject><subject>Cell Transformation, Neoplastic</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>DNA, Circular - isolation & purification</subject><subject>DNA, Mitochondrial - isolation & purification</subject><subject>DNA, Neoplasm</subject><subject>DNA, Viral</subject><subject>Ethidium</subject><subject>Rats</subject><subject>Sarcoma, Experimental - microbiology</subject><subject>Sarcoma, Experimental - transmission</subject><subject>Transformation, Genetic</subject><issn>0014-2964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhj3wVT7-QYdMCIaA7diOvSBVhQJSBQtIbJZjO6pREhfbAfHvSdoKNqaT7t73vbsHgCmCVwgidg0hIjkWjFyU9FJACGlO98Dkt30EjmN8hxBzQvEhOECMccImgC-Ua_pgs-QzXyXluiwF1cXa6uR8l325tMre5lnrktcr35ngVJPdPs1OwX6tmmjPdvUEvC7uXuYP-fL5_nE-W-a6oCzlNS9KgYTGpSVIUY4FtByKAlPIamN4yYyBTCExnEMQ1GwQVBUqMNfYVIUoTsD5Nncd_EdvY5Kti9o2jeqs76PkmI8LRiHZCnXwMQZby3VwrQrfEkE5MpIjDDnCkCWVG0aSDrbpLr-vWmv-TBtAw_hmO7bDj5_OBhm1s522xoUBkTTe_Z__A4QVdSk</recordid><startdate>197504</startdate><enddate>197504</enddate><creator>Svoboda, Jan</creator><creator>Hložánek, Ivo</creator><creator>Korb, Jan</creator><creator>Mach, Otakar</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197504</creationdate><title>Failure to obtain transfection with XC mitochondrial DNA</title><author>Svoboda, Jan ; Hložánek, Ivo ; Korb, Jan ; Mach, Otakar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-f837919c27e41a58290e80932506fdd876dd06a19684410c6290bb1328c2db393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Avian Sarcoma Viruses</topic><topic>Cell Line</topic><topic>Cell Transformation, Neoplastic</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>DNA, Circular - isolation & purification</topic><topic>DNA, Mitochondrial - isolation & purification</topic><topic>DNA, Neoplasm</topic><topic>DNA, Viral</topic><topic>Ethidium</topic><topic>Rats</topic><topic>Sarcoma, Experimental - microbiology</topic><topic>Sarcoma, Experimental - transmission</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Svoboda, Jan</creatorcontrib><creatorcontrib>Hložánek, Ivo</creatorcontrib><creatorcontrib>Korb, Jan</creatorcontrib><creatorcontrib>Mach, Otakar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Svoboda, Jan</au><au>Hložánek, Ivo</au><au>Korb, Jan</au><au>Mach, Otakar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Failure to obtain transfection with XC mitochondrial DNA</atitle><jtitle>European journal of cancer</jtitle><addtitle>Eur J Cancer</addtitle><date>1975-04</date><risdate>1975</risdate><volume>11</volume><issue>4</issue><spage>247,IN5</spage><epage>250,IN5</epage><pages>247,IN5-250,IN5</pages><issn>0014-2964</issn><abstract>The transfecting activity of DNA obtained from mitochondria of XC virogenic rat cells transformed with the Prague strain of RSV was tested. Both mitochondrial DNA isolated with EthBr, containing circular molecules, and total mitochondrial DNA were inefficient in transfecting sensitive chicken fibroblasts in spite of the fact that doses of mitochondrial DNA up to two orders higher than the minimum efficient dose of total XC DNA were used. These findings support the view that a DNA transcript of the RSV genome is not integrated in mitochondrial DNA.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>166846</pmid><doi>10.1016/0014-2964(75)90005-5</doi><tpages>4</tpages></addata></record> |
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| ispartof | European journal of cancer, 1975-04, Vol.11 (4), p.247,IN5-250,IN5 |
| issn | 0014-2964 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82879199 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Avian Sarcoma Viruses Cell Line Cell Transformation, Neoplastic Chick Embryo Chickens DNA, Circular - isolation & purification DNA, Mitochondrial - isolation & purification DNA, Neoplasm DNA, Viral Ethidium Rats Sarcoma, Experimental - microbiology Sarcoma, Experimental - transmission Transformation, Genetic |
| title | Failure to obtain transfection with XC mitochondrial DNA |
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