Cell surface charge and regulation of cell division of 3T3 cells and transformed derivatives
The cell surface charge of 3T3, 3T6, SV40-3T3 cells and trypsin-, neuraminidase- and serumtreated preparations of these has been characterized by microcell electrophoresis. At 25 °C, density-inhibited 3T3 cells show a decrease in electrophoretic mobility when treated with various stimuli of cell div...
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| Veröffentlicht in: | Experimental cell research 1975-06, Vol.93 (1), p.71-78 |
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| container_title | Experimental cell research |
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| creator | Adam, Gerburg Adam, Gerold |
| description | The cell surface charge of 3T3, 3T6, SV40-3T3 cells and trypsin-, neuraminidase- and serumtreated preparations of these has been characterized by microcell electrophoresis. At 25 °C, density-inhibited 3T3 cells show a decrease in electrophoretic mobility when treated with various stimuli of cell division. This effect is not observed at 25 °C for transformed derivatives. The surface charge configuration of various cell preparations exhibits a thermal transition which is located within a temperature range characteristic of each preparation. These and other results from cell electrophoresis, taken together with those obtained in agglutination studies by other authors, are considered evidence for the occurrence in the plasma membrane of these cells of a twodimensional phase separation. The temperature range of this phase separation is shifted on treating the cells by growth stimuli. This effect might be an indication of a basic trigger mechanism in the cell cycle. |
| doi_str_mv | 10.1016/0014-4827(75)90424-3 |
| format | Article |
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At 25 °C, density-inhibited 3T3 cells show a decrease in electrophoretic mobility when treated with various stimuli of cell division. This effect is not observed at 25 °C for transformed derivatives. The surface charge configuration of various cell preparations exhibits a thermal transition which is located within a temperature range characteristic of each preparation. These and other results from cell electrophoresis, taken together with those obtained in agglutination studies by other authors, are considered evidence for the occurrence in the plasma membrane of these cells of a twodimensional phase separation. The temperature range of this phase separation is shifted on treating the cells by growth stimuli. This effect might be an indication of a basic trigger mechanism in the cell cycle.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(75)90424-3</identifier><identifier>PMID: 166856</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Calcium - pharmacology ; Cell Division ; Cell Line ; Cell Membrane - drug effects ; Cell Membrane - physiology ; Cell Transformation, Neoplastic ; Electrophoresis ; Electrophysiology ; Fibroblasts ; Lectins - pharmacology ; Mice ; Mice, Inbred BALB C ; Neuraminidase - pharmacology ; Plasma - drug effects ; Receptors, Drug ; Simian virus 40 ; Surface Properties ; Temperature ; Trypsin - pharmacology</subject><ispartof>Experimental cell research, 1975-06, Vol.93 (1), p.71-78</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-3f3a468af4e140e547977cd7e1a2e70329d965872a8acfc85ba34614ecfb81d03</citedby><cites>FETCH-LOGICAL-c356t-3f3a468af4e140e547977cd7e1a2e70329d965872a8acfc85ba34614ecfb81d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(75)90424-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/166856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adam, Gerburg</creatorcontrib><creatorcontrib>Adam, Gerold</creatorcontrib><title>Cell surface charge and regulation of cell division of 3T3 cells and transformed derivatives</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The cell surface charge of 3T3, 3T6, SV40-3T3 cells and trypsin-, neuraminidase- and serumtreated preparations of these has been characterized by microcell electrophoresis. At 25 °C, density-inhibited 3T3 cells show a decrease in electrophoretic mobility when treated with various stimuli of cell division. This effect is not observed at 25 °C for transformed derivatives. The surface charge configuration of various cell preparations exhibits a thermal transition which is located within a temperature range characteristic of each preparation. These and other results from cell electrophoresis, taken together with those obtained in agglutination studies by other authors, are considered evidence for the occurrence in the plasma membrane of these cells of a twodimensional phase separation. The temperature range of this phase separation is shifted on treating the cells by growth stimuli. This effect might be an indication of a basic trigger mechanism in the cell cycle.</description><subject>Animals</subject><subject>Calcium - pharmacology</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - physiology</subject><subject>Cell Transformation, Neoplastic</subject><subject>Electrophoresis</subject><subject>Electrophysiology</subject><subject>Fibroblasts</subject><subject>Lectins - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neuraminidase - pharmacology</subject><subject>Plasma - drug effects</subject><subject>Receptors, Drug</subject><subject>Simian virus 40</subject><subject>Surface Properties</subject><subject>Temperature</subject><subject>Trypsin - pharmacology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoN4q9U36GJWoovR3CbJbAQp3qDgpu6EkCYnNdLO1GRmwLd3pi26cxU45_t_cj6EJgTfEEzELcaE51xReSWL6xJzynN2gEYElzinnNJDNPpFTtFZSp8YY6WIOEHHRAhViBF6n8JqlaU2emMhsx8mLiEzlcsiLNuVaUJdZbXP7EC50IW0H7A52w7TFm6iqZKv4xpc5iCGrg92kM7RkTerBBf7d4zeHh_m0-d89vr0Mr2f5ZYVosmZZ4YLZTwHwjEUXJZSWieBGAoSM1q6UhRKUqOM9VYVC8O4IBysXyjiMBujy13vJtZfLaRGr0MafmcqqNukFVVSlHgA-Q60sU4pgtebGNYmfmuC9eBUD8L0IEzLQm-datbHJvv-dtGf-BfaSuzXd7s19Dd2AaJONkBlwYUIttGuDv_3_wDBlIWT</recordid><startdate>197506</startdate><enddate>197506</enddate><creator>Adam, Gerburg</creator><creator>Adam, Gerold</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197506</creationdate><title>Cell surface charge and regulation of cell division of 3T3 cells and transformed derivatives</title><author>Adam, Gerburg ; Adam, Gerold</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-3f3a468af4e140e547977cd7e1a2e70329d965872a8acfc85ba34614ecfb81d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Calcium - pharmacology</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - physiology</topic><topic>Cell Transformation, Neoplastic</topic><topic>Electrophoresis</topic><topic>Electrophysiology</topic><topic>Fibroblasts</topic><topic>Lectins - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neuraminidase - pharmacology</topic><topic>Plasma - drug effects</topic><topic>Receptors, Drug</topic><topic>Simian virus 40</topic><topic>Surface Properties</topic><topic>Temperature</topic><topic>Trypsin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adam, Gerburg</creatorcontrib><creatorcontrib>Adam, Gerold</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adam, Gerburg</au><au>Adam, Gerold</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell surface charge and regulation of cell division of 3T3 cells and transformed derivatives</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1975-06</date><risdate>1975</risdate><volume>93</volume><issue>1</issue><spage>71</spage><epage>78</epage><pages>71-78</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>The cell surface charge of 3T3, 3T6, SV40-3T3 cells and trypsin-, neuraminidase- and serumtreated preparations of these has been characterized by microcell electrophoresis. At 25 °C, density-inhibited 3T3 cells show a decrease in electrophoretic mobility when treated with various stimuli of cell division. This effect is not observed at 25 °C for transformed derivatives. The surface charge configuration of various cell preparations exhibits a thermal transition which is located within a temperature range characteristic of each preparation. These and other results from cell electrophoresis, taken together with those obtained in agglutination studies by other authors, are considered evidence for the occurrence in the plasma membrane of these cells of a twodimensional phase separation. The temperature range of this phase separation is shifted on treating the cells by growth stimuli. This effect might be an indication of a basic trigger mechanism in the cell cycle.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>166856</pmid><doi>10.1016/0014-4827(75)90424-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | Experimental cell research, 1975-06, Vol.93 (1), p.71-78 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Calcium - pharmacology Cell Division Cell Line Cell Membrane - drug effects Cell Membrane - physiology Cell Transformation, Neoplastic Electrophoresis Electrophysiology Fibroblasts Lectins - pharmacology Mice Mice, Inbred BALB C Neuraminidase - pharmacology Plasma - drug effects Receptors, Drug Simian virus 40 Surface Properties Temperature Trypsin - pharmacology |
| title | Cell surface charge and regulation of cell division of 3T3 cells and transformed derivatives |
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