Separation and Determination of Carnitine and Its Esters in Human Serum
A colorimetric method for the deter-mination of carnitine and its derivatives is described. Free carnitine, acetylcarni-tine and palmitoylcarnitine were extracted with chloroform-methanol. After evaporation, the residue was dissolved in n-butanol and water; palmitoylcarnitine in the organic phase, a...
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| Veröffentlicht in: | The Tohoku Journal of Experimental Medicine 1975, Vol.116(2), pp.203-204 |
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| container_end_page | 204 |
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| container_issue | 2 |
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| container_title | The Tohoku Journal of Experimental Medicine |
| container_volume | 116 |
| creator | MAEBASHI, MASARU KAWAMURA, NORIMITSU SATO, MITSUO YOSHINAGA, KAORU SUZUKI, MASASHIGE |
| description | A colorimetric method for the deter-mination of carnitine and its derivatives is described. Free carnitine, acetylcarni-tine and palmitoylcarnitine were extracted with chloroform-methanol. After evaporation, the residue was dissolved in n-butanol and water; palmitoylcarnitine in the organic phase, and free carnitine and acetylcarnitine in the aqueous phase. Acetylcarnitine was separated from free carnitine by passing the aqueous phase through a column of Amberlite CG-120 (H+). Separated carnitine derivatives were hydrolyzed to free carnitine which was followed by the Amberlite column chromatography. After eluting with 2N NH4OH solution, the samples were esterified and determined colorimetrically. |
| doi_str_mv | 10.1620/tjem.116.203 |
| format | Article |
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Free carnitine, acetylcarni-tine and palmitoylcarnitine were extracted with chloroform-methanol. After evaporation, the residue was dissolved in n-butanol and water; palmitoylcarnitine in the organic phase, and free carnitine and acetylcarnitine in the aqueous phase. Acetylcarnitine was separated from free carnitine by passing the aqueous phase through a column of Amberlite CG-120 (H+). Separated carnitine derivatives were hydrolyzed to free carnitine which was followed by the Amberlite column chromatography. After eluting with 2N NH4OH solution, the samples were esterified and determined colorimetrically.</description><identifier>ISSN: 0040-8727</identifier><identifier>EISSN: 1349-3329</identifier><identifier>DOI: 10.1620/tjem.116.203</identifier><identifier>PMID: 1154389</identifier><language>eng</language><publisher>Japan: Tohoku University Medical Press</publisher><subject>acetylcarnitine ; carnitine ; Carnitine - blood ; Colorimetry - methods ; Esters - blood ; Female ; Humans ; Male ; Methods ; palmitoylcarnitine</subject><ispartof>The Tohoku Journal of Experimental Medicine, 1975, Vol.116(2), pp.203-204</ispartof><rights>Tohoku University Medical Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c579t-add3bc0766eb985e6b0e72d6f7127c38d74cae732ad34d3c29f0d057f4180eb93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1154389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MAEBASHI, MASARU</creatorcontrib><creatorcontrib>KAWAMURA, NORIMITSU</creatorcontrib><creatorcontrib>SATO, MITSUO</creatorcontrib><creatorcontrib>YOSHINAGA, KAORU</creatorcontrib><creatorcontrib>SUZUKI, MASASHIGE</creatorcontrib><title>Separation and Determination of Carnitine and Its Esters in Human Serum</title><title>The Tohoku Journal of Experimental Medicine</title><addtitle>Tohoku J. Exp. Med.</addtitle><description>A colorimetric method for the deter-mination of carnitine and its derivatives is described. Free carnitine, acetylcarni-tine and palmitoylcarnitine were extracted with chloroform-methanol. After evaporation, the residue was dissolved in n-butanol and water; palmitoylcarnitine in the organic phase, and free carnitine and acetylcarnitine in the aqueous phase. Acetylcarnitine was separated from free carnitine by passing the aqueous phase through a column of Amberlite CG-120 (H+). Separated carnitine derivatives were hydrolyzed to free carnitine which was followed by the Amberlite column chromatography. After eluting with 2N NH4OH solution, the samples were esterified and determined colorimetrically.</description><subject>acetylcarnitine</subject><subject>carnitine</subject><subject>Carnitine - blood</subject><subject>Colorimetry - methods</subject><subject>Esters - blood</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Methods</subject><subject>palmitoylcarnitine</subject><issn>0040-8727</issn><issn>1349-3329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtPwzAURi0EglLYWJEyMZHiRxInIyp9SZUYCrPl2DfgqnGK7Qz8e9ymKouv5O_co6sPoQeCJ6Sg-CVsoZ0QUkwoZhdoRFhWpYzR6hKNMM5wWnLKb9Ct91uMWYZ5cY2uCckzVlYjtNjAXjoZTGcTaXXyBgFca-zw0zXJVDprgrFwjFfBJzMfEZ8Ymyz7VtpkA65v79BVI3ce7k9zjD7ns4_pMl2_L1bT13Wqcl6FVGrNahVvKKCuyhyKGgOnumg4oVyxUvNMSeCMSs0yzRStGqxxzpuMlDiusDF6Grx71_304INojVew20kLXe9FScs841URwecBVK7z3kEj9s600v0KgsWhN3HoTcTeROwt4o8nb1-3oP_hoaiYz4d864P8gnMuXTBqB0cZqaL1KDy9UXwG1Ld0Aiz7A9HlgXY</recordid><startdate>19750101</startdate><enddate>19750101</enddate><creator>MAEBASHI, MASARU</creator><creator>KAWAMURA, NORIMITSU</creator><creator>SATO, MITSUO</creator><creator>YOSHINAGA, KAORU</creator><creator>SUZUKI, MASASHIGE</creator><general>Tohoku University Medical Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750101</creationdate><title>Separation and Determination of Carnitine and Its Esters in Human Serum</title><author>MAEBASHI, MASARU ; KAWAMURA, NORIMITSU ; SATO, MITSUO ; YOSHINAGA, KAORU ; SUZUKI, MASASHIGE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c579t-add3bc0766eb985e6b0e72d6f7127c38d74cae732ad34d3c29f0d057f4180eb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>acetylcarnitine</topic><topic>carnitine</topic><topic>Carnitine - blood</topic><topic>Colorimetry - methods</topic><topic>Esters - blood</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Methods</topic><topic>palmitoylcarnitine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAEBASHI, MASARU</creatorcontrib><creatorcontrib>KAWAMURA, NORIMITSU</creatorcontrib><creatorcontrib>SATO, MITSUO</creatorcontrib><creatorcontrib>YOSHINAGA, KAORU</creatorcontrib><creatorcontrib>SUZUKI, MASASHIGE</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Tohoku Journal of Experimental Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MAEBASHI, MASARU</au><au>KAWAMURA, NORIMITSU</au><au>SATO, MITSUO</au><au>YOSHINAGA, KAORU</au><au>SUZUKI, MASASHIGE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and Determination of Carnitine and Its Esters in Human Serum</atitle><jtitle>The Tohoku Journal of Experimental Medicine</jtitle><addtitle>Tohoku J. Exp. Med.</addtitle><date>1975-01-01</date><risdate>1975</risdate><volume>116</volume><issue>2</issue><spage>203</spage><epage>204</epage><pages>203-204</pages><issn>0040-8727</issn><eissn>1349-3329</eissn><abstract>A colorimetric method for the deter-mination of carnitine and its derivatives is described. Free carnitine, acetylcarni-tine and palmitoylcarnitine were extracted with chloroform-methanol. After evaporation, the residue was dissolved in n-butanol and water; palmitoylcarnitine in the organic phase, and free carnitine and acetylcarnitine in the aqueous phase. Acetylcarnitine was separated from free carnitine by passing the aqueous phase through a column of Amberlite CG-120 (H+). Separated carnitine derivatives were hydrolyzed to free carnitine which was followed by the Amberlite column chromatography. After eluting with 2N NH4OH solution, the samples were esterified and determined colorimetrically.</abstract><cop>Japan</cop><pub>Tohoku University Medical Press</pub><pmid>1154389</pmid><doi>10.1620/tjem.116.203</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Tohoku Journal of Experimental Medicine, 1975, Vol.116(2), pp.203-204 |
| issn | 0040-8727 1349-3329 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | acetylcarnitine carnitine Carnitine - blood Colorimetry - methods Esters - blood Female Humans Male Methods palmitoylcarnitine |
| title | Separation and Determination of Carnitine and Its Esters in Human Serum |
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