[10] Autoradiographic techniques for localizing protein hormones in target tissue
Autoradiography is an extremely useful method for determining the distribution of radioactive compounds including steroid, protein, and peptide hormones at the level of the organ, tissue, or cell. The method involves coating thin tissue sections containing radioactive hormones with photographic emul...
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| Veröffentlicht in: | Methods in Enzymology 1975, Vol.37, p.145-167 |
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| container_title | Methods in Enzymology |
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| creator | Rajaniemi, Hannu J. Rees Midgley, A. |
| description | Autoradiography is an extremely useful method for determining the distribution of radioactive compounds including steroid, protein, and peptide hormones at the level of the organ, tissue, or cell. The method involves coating thin tissue sections containing radioactive hormones with photographic emulsion, exposing the emulsion for variable times, and then converting the invisible latent images into visible silver grains. The distribution of revealed radioactivity can be correlated with histological and histochemieal results obtained with adjacent serial sections. Although autoradiographic methods have improved steadily over the past several years, advances in protein hormone autoradiography have been slow. With the recent development of gentle methods for preparing biologically active, labeled protein hormones, this situation has changed and numerous autoradiographic studies concerning the localization and mechanism of action of labeled protein hormones have appeared. |
| doi_str_mv | 10.1016/S0076-6879(75)37012-2 |
| format | Article |
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With the recent development of gentle methods for preparing biologically active, labeled protein hormones, this situation has changed and numerous autoradiographic studies concerning the localization and mechanism of action of labeled protein hormones have appeared.</description><subject>Animals</subject><subject>Autoradiography - methods</subject><subject>Female</subject><subject>Fetus</subject><subject>Hormones - analysis</subject><subject>Iodine Radioisotopes</subject><subject>Isotope Labeling</subject><subject>Kidney Cortex - analysis</subject><subject>Liver - analysis</subject><subject>Lung - analysis</subject><subject>Mice</subject><subject>Organ Specificity</subject><subject>Peptides - analysis</subject><subject>Placenta - analysis</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Tritium</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>012181937X</isbn><isbn>9780121819378</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UdtKAzEQDV6otfYTCvsk-rCa2dtkn6QUbyCIqCCIhDQ7bSPbTU2ygn692wvOywznHIbDOYyNgF8Ah-LymXMs4kJgeYb5eYockjjZY33Ic4yxFGKfHXcYCChTfDtg_X_9ERt6_8m7yRFTznusB5CIJEv77Okd-Ec0boN1qjJ27tRqYXQUSC8a89WSj2bWRbXVqja_pplHK2cDmSZaWLe0Tcd3d1BuTiEKxvuWTtjhTNWehrs9YK831y-Tu_jh8fZ-Mn6ICRBDjFkBpDlQJTIBJApV0ZS4Qq51immmVSJ0mec0RaGTAgpdFqXKBOcVzGYk0gE73f7tHK2NBrk0XlNdq4Zs66VIRFZimXXC0U7YTpdUyZUzS-V-5C6Djr_a8tS5_TbkpNeGGk2VcaSDrKyRwOW6BLkpQa5DlZjLTQkySf8A4nd26g</recordid><startdate>1975</startdate><enddate>1975</enddate><creator>Rajaniemi, Hannu J.</creator><creator>Rees Midgley, A.</creator><general>Elsevier Science & Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1975</creationdate><title>[10] Autoradiographic techniques for localizing protein hormones in target tissue</title><author>Rajaniemi, Hannu J. ; Rees Midgley, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e177t-7461ec01ed8481e86adebe0a70cc3734ca28c955eb78c2616c969a4800d1ffe83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Autoradiography - methods</topic><topic>Female</topic><topic>Fetus</topic><topic>Hormones - analysis</topic><topic>Iodine Radioisotopes</topic><topic>Isotope Labeling</topic><topic>Kidney Cortex - analysis</topic><topic>Liver - analysis</topic><topic>Lung - analysis</topic><topic>Mice</topic><topic>Organ Specificity</topic><topic>Peptides - analysis</topic><topic>Placenta - analysis</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajaniemi, Hannu J.</creatorcontrib><creatorcontrib>Rees Midgley, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajaniemi, Hannu J.</au><au>Rees Midgley, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[10] Autoradiographic techniques for localizing protein hormones in target tissue</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>1975</date><risdate>1975</risdate><volume>37</volume><spage>145</spage><epage>167</epage><pages>145-167</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>012181937X</isbn><isbn>9780121819378</isbn><abstract>Autoradiography is an extremely useful method for determining the distribution of radioactive compounds including steroid, protein, and peptide hormones at the level of the organ, tissue, or cell. The method involves coating thin tissue sections containing radioactive hormones with photographic emulsion, exposing the emulsion for variable times, and then converting the invisible latent images into visible silver grains. The distribution of revealed radioactivity can be correlated with histological and histochemieal results obtained with adjacent serial sections. Although autoradiographic methods have improved steadily over the past several years, advances in protein hormone autoradiography have been slow. With the recent development of gentle methods for preparing biologically active, labeled protein hormones, this situation has changed and numerous autoradiographic studies concerning the localization and mechanism of action of labeled protein hormones have appeared.</abstract><cop>United States</cop><pub>Elsevier Science & Technology</pub><pmid>1128243</pmid><doi>10.1016/S0076-6879(75)37012-2</doi><tpages>23</tpages></addata></record> |
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| ispartof | Methods in Enzymology, 1975, Vol.37, p.145-167 |
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| language | eng |
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| source | MEDLINE; ScienceDirect eBooks; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Autoradiography - methods Female Fetus Hormones - analysis Iodine Radioisotopes Isotope Labeling Kidney Cortex - analysis Liver - analysis Lung - analysis Mice Organ Specificity Peptides - analysis Placenta - analysis Pregnancy Rats Tritium |
| title | [10] Autoradiographic techniques for localizing protein hormones in target tissue |
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