Purification of yeast alpha-isopropylmalate isomerase. High ionic strength hydrophobic chromatography

alpha-Isopropylmalate isomerase, the second enzyme specific for leucine biosynthesis, can be purified from extracts of yeast utilizing a chromatographic procedure that allows separation of proteins in the presence of high concentrations of (NH4)2SO4. The purification procedure utilizes the stabilizi...

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Veröffentlicht in:	The Journal of biological chemistry 1975-06, Vol.250 (11), p.4315-4321
Hauptverfasser:	Bigelis, R, Umbarger, H E
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Affinity Electrophoresis, Disc Hydro-Lyases Keto Acids Malates Osmolar Concentration Saccharomyces - enzymology Time Factors Ultrafiltration
Online-Zugang:	Volltext
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