A liver factor that interconverts multiple forms of tyrosine aminotransferase
Three activity peaks of rat liver soluble tyrosine aminotransferase have been resolved using hydroxyl-apatite chromatography. These peaks interconvert during storage of the soluble enzyme preparation in ice for 20 h. A component of a particulate fraction of liver which will interconvert the forms of...
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| Veröffentlicht in: | Life sciences (1973) 1975-02, Vol.16 (3), p.437-449 |
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| container_end_page | 449 |
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| container_title | Life sciences (1973) |
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| creator | Smith, Garry J. Pearce, P.Helen Oliver, Ivan T. |
| description | Three activity peaks of rat liver soluble tyrosine aminotransferase have been resolved using hydroxyl-apatite chromatography. These peaks interconvert during storage of the soluble enzyme preparation in ice for 20 h. A component of a particulate fraction of liver which will interconvert the forms of tyrosine aminotransferase
in vitro with no alteration of total enzyme activity has been detected. This factor is present in a 31, 000 gh pellet of liver and is solubilized by sonication. When the factor is subjected to dialysis or incubation at 25°C for 30 min. its effect on tyrosine aminotransferase is greatly diminished. |
| doi_str_mv | 10.1016/0024-3205(75)90265-9 |
| format | Article |
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in vitro with no alteration of total enzyme activity has been detected. This factor is present in a 31, 000 gh pellet of liver and is solubilized by sonication. When the factor is subjected to dialysis or incubation at 25°C for 30 min. its effect on tyrosine aminotransferase is greatly diminished.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(75)90265-9</identifier><identifier>PMID: 236421</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Aspartate Aminotransferases - metabolism ; Cell Fractionation ; Chromatography ; Cytosol - enzymology ; Enzyme Induction - drug effects ; Hydroxyapatites ; Liver - enzymology ; Mitochondria, Liver - enzymology ; Rats ; Sonication ; Tissue Extracts - pharmacology ; Tyrosine Transaminase - isolation & purification ; Tyrosine Transaminase - metabolism ; Ultracentrifugation</subject><ispartof>Life sciences (1973), 1975-02, Vol.16 (3), p.437-449</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-c15d9ab1bde3539d002616c1143f8ac23355f6458f8bcc4fe08f6245e5b01ab3</citedby><cites>FETCH-LOGICAL-c356t-c15d9ab1bde3539d002616c1143f8ac23355f6458f8bcc4fe08f6245e5b01ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0024-3205(75)90265-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/236421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Garry J.</creatorcontrib><creatorcontrib>Pearce, P.Helen</creatorcontrib><creatorcontrib>Oliver, Ivan T.</creatorcontrib><title>A liver factor that interconverts multiple forms of tyrosine aminotransferase</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Three activity peaks of rat liver soluble tyrosine aminotransferase have been resolved using hydroxyl-apatite chromatography. These peaks interconvert during storage of the soluble enzyme preparation in ice for 20 h. A component of a particulate fraction of liver which will interconvert the forms of tyrosine aminotransferase
in vitro with no alteration of total enzyme activity has been detected. This factor is present in a 31, 000 gh pellet of liver and is solubilized by sonication. When the factor is subjected to dialysis or incubation at 25°C for 30 min. its effect on tyrosine aminotransferase is greatly diminished.</description><subject>Animals</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>Cell Fractionation</subject><subject>Chromatography</subject><subject>Cytosol - enzymology</subject><subject>Enzyme Induction - drug effects</subject><subject>Hydroxyapatites</subject><subject>Liver - enzymology</subject><subject>Mitochondria, Liver - enzymology</subject><subject>Rats</subject><subject>Sonication</subject><subject>Tissue Extracts - pharmacology</subject><subject>Tyrosine Transaminase - isolation & purification</subject><subject>Tyrosine Transaminase - metabolism</subject><subject>Ultracentrifugation</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC3EqxT-QQ8-ITgE_Iid5IJUVbykIi69W46zFkZJXGynUv89Ka165LTS7sxo9kNoRskDJVQ-EsLyjDMi7gpxXxEmRVadoAktiyojktNTNDlKLtFVjN-EECEKfoHOGZc5oxP0Mcet20DAVpvkA05fOmHXJwjG9-M-RdwNbXLrFrD1oYvYW5y2wUfXA9ad630Kuo8Wgo5wjc6sbiPcHOYUrV6eV4u3bPn5-r6YLzPDhUyZoaKpdE3rBrjgVTO2lFQaSnNuS20Y50JYmYvSlrUxuQVSWslyAaImVNd8im73sevgfwaISXUuGmhb3YMfoipZyYuKlqMw3wvNWDgGsGodXKfDVlGidgzVDpDaAVKFUH8MVTXaZof8oe6gOZr20Mbz0_4M44sbB0FF46A30LgAJqnGu__zfwEThIEg</recordid><startdate>19750201</startdate><enddate>19750201</enddate><creator>Smith, Garry J.</creator><creator>Pearce, P.Helen</creator><creator>Oliver, Ivan T.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750201</creationdate><title>A liver factor that interconverts multiple forms of tyrosine aminotransferase</title><author>Smith, Garry J. ; Pearce, P.Helen ; Oliver, Ivan T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-c15d9ab1bde3539d002616c1143f8ac23355f6458f8bcc4fe08f6245e5b01ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Aspartate Aminotransferases - metabolism</topic><topic>Cell Fractionation</topic><topic>Chromatography</topic><topic>Cytosol - enzymology</topic><topic>Enzyme Induction - drug effects</topic><topic>Hydroxyapatites</topic><topic>Liver - enzymology</topic><topic>Mitochondria, Liver - enzymology</topic><topic>Rats</topic><topic>Sonication</topic><topic>Tissue Extracts - pharmacology</topic><topic>Tyrosine Transaminase - isolation & purification</topic><topic>Tyrosine Transaminase - metabolism</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Garry J.</creatorcontrib><creatorcontrib>Pearce, P.Helen</creatorcontrib><creatorcontrib>Oliver, Ivan T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Garry J.</au><au>Pearce, P.Helen</au><au>Oliver, Ivan T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A liver factor that interconverts multiple forms of tyrosine aminotransferase</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1975-02-01</date><risdate>1975</risdate><volume>16</volume><issue>3</issue><spage>437</spage><epage>449</epage><pages>437-449</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Three activity peaks of rat liver soluble tyrosine aminotransferase have been resolved using hydroxyl-apatite chromatography. These peaks interconvert during storage of the soluble enzyme preparation in ice for 20 h. A component of a particulate fraction of liver which will interconvert the forms of tyrosine aminotransferase
in vitro with no alteration of total enzyme activity has been detected. This factor is present in a 31, 000 gh pellet of liver and is solubilized by sonication. When the factor is subjected to dialysis or incubation at 25°C for 30 min. its effect on tyrosine aminotransferase is greatly diminished.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>236421</pmid><doi>10.1016/0024-3205(75)90265-9</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 1975-02, Vol.16 (3), p.437-449 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82837918 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Aspartate Aminotransferases - metabolism Cell Fractionation Chromatography Cytosol - enzymology Enzyme Induction - drug effects Hydroxyapatites Liver - enzymology Mitochondria, Liver - enzymology Rats Sonication Tissue Extracts - pharmacology Tyrosine Transaminase - isolation & purification Tyrosine Transaminase - metabolism Ultracentrifugation |
| title | A liver factor that interconverts multiple forms of tyrosine aminotransferase |
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