Distribution of acidic glycosaminoglycans in the intima, media and adventitia of bovine aorta and their anticoagulant properties
1. 1. The constituents of acidic glycosaminoglycans in the tunica intima, media and adventitia were studied by means of an enzymatic assay with chondroitinases and by electrophoretic characterization. 2. 2. The content of the acidic glycosaminoglycans was higher in the intima in the adventitia based...
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| Veröffentlicht in: | Atherosclerosis 1975, Vol.21 (1), p.93-103 |
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| container_title | Atherosclerosis |
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| creator | Murata, Katsumi Nakazawa, Koji Hamai, Akio |
| description | 1.
1. The constituents of acidic glycosaminoglycans in the tunica intima, media and adventitia were studied by means of an enzymatic assay with chondroitinases and by electrophoretic characterization.
2.
2. The content of the acidic glycosaminoglycans was higher in the intima in the adventitia based on defatted dry tissue weight. filtration pattern showed that, with chondroitinase-AC, the proportion of the intact glycosaminoglycans is greater in the adventitia than in the intima.
3.
3. Electrophoresis before and after digestion with chondroitinases indicated that the three layers contain chondroitin-4- and -6-sulfates, dermatan sulfates heparan sulfates and hyaluronic acid. In the case of the adventitia, bands migrating like the heparin standard were also detected.
4.
4. Paper chromatographic separation of the unsaturated disaccharides after digestion with chondroitinases revealed that the proportion of chondroitin-6-sulfate is predominant in the intima, whereas the proportion of heparan sulfates, hyaluronic acid and dermatan sulfate is
2
1
2
−2
times higher in the adventitia than in the intima.
5.
5. Anticoagulant activity, measured by thrombelastography, of the acidic glycosaminoglycans in the three layers clearly indicated that the glycosaminoglycans in the adventitia possess the highest potency among the three layers. It was considered that the higher activity of the glycosaminoglycans in the adventitia is due to the relatively greater proportion of heparan sulfates and dermatan sulfate. |
| doi_str_mv | 10.1016/0021-9150(75)90096-9 |
| format | Article |
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1. The constituents of acidic glycosaminoglycans in the tunica intima, media and adventitia were studied by means of an enzymatic assay with chondroitinases and by electrophoretic characterization.
2.
2. The content of the acidic glycosaminoglycans was higher in the intima in the adventitia based on defatted dry tissue weight. filtration pattern showed that, with chondroitinase-AC, the proportion of the intact glycosaminoglycans is greater in the adventitia than in the intima.
3.
3. Electrophoresis before and after digestion with chondroitinases indicated that the three layers contain chondroitin-4- and -6-sulfates, dermatan sulfates heparan sulfates and hyaluronic acid. In the case of the adventitia, bands migrating like the heparin standard were also detected.
4.
4. Paper chromatographic separation of the unsaturated disaccharides after digestion with chondroitinases revealed that the proportion of chondroitin-6-sulfate is predominant in the intima, whereas the proportion of heparan sulfates, hyaluronic acid and dermatan sulfate is
2
1
2
−2
times higher in the adventitia than in the intima.
5.
5. Anticoagulant activity, measured by thrombelastography, of the acidic glycosaminoglycans in the three layers clearly indicated that the glycosaminoglycans in the adventitia possess the highest potency among the three layers. It was considered that the higher activity of the glycosaminoglycans in the adventitia is due to the relatively greater proportion of heparan sulfates and dermatan sulfate.</description><identifier>ISSN: 0021-9150</identifier><identifier>EISSN: 1879-1484</identifier><identifier>DOI: 10.1016/0021-9150(75)90096-9</identifier><identifier>PMID: 124175</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Acidic glycosaminoglycans ; Animals ; Anticoagulant activity ; Aorta - analysis ; Arterial wall ; Blood Coagulation - drug effects ; Cattle ; Dermatan sulfate ; Dermatan Sulfate - analysis ; Electrophoresis ; Glycosaminoglycans - analysis ; Glycosaminoglycans - pharmacology ; Heparan Sulfate ; Heparin ; Heparin - analysis ; Heparitin Sulfate - analysis ; Hyaluronic acid ; Hyaluronic Acid - analysis ; Thrombelastography</subject><ispartof>Atherosclerosis, 1975, Vol.21 (1), p.93-103</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-dfaf0fe639e80ba16dbfb2a517b4e74bb2fdde4adbdd39bc0036cd6c6eda9db13</citedby><cites>FETCH-LOGICAL-c356t-dfaf0fe639e80ba16dbfb2a517b4e74bb2fdde4adbdd39bc0036cd6c6eda9db13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0021915075900969$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/124175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murata, Katsumi</creatorcontrib><creatorcontrib>Nakazawa, Koji</creatorcontrib><creatorcontrib>Hamai, Akio</creatorcontrib><title>Distribution of acidic glycosaminoglycans in the intima, media and adventitia of bovine aorta and their anticoagulant properties</title><title>Atherosclerosis</title><addtitle>Atherosclerosis</addtitle><description>1.
1. The constituents of acidic glycosaminoglycans in the tunica intima, media and adventitia were studied by means of an enzymatic assay with chondroitinases and by electrophoretic characterization.
2.
2. The content of the acidic glycosaminoglycans was higher in the intima in the adventitia based on defatted dry tissue weight. filtration pattern showed that, with chondroitinase-AC, the proportion of the intact glycosaminoglycans is greater in the adventitia than in the intima.
3.
3. Electrophoresis before and after digestion with chondroitinases indicated that the three layers contain chondroitin-4- and -6-sulfates, dermatan sulfates heparan sulfates and hyaluronic acid. In the case of the adventitia, bands migrating like the heparin standard were also detected.
4.
4. Paper chromatographic separation of the unsaturated disaccharides after digestion with chondroitinases revealed that the proportion of chondroitin-6-sulfate is predominant in the intima, whereas the proportion of heparan sulfates, hyaluronic acid and dermatan sulfate is
2
1
2
−2
times higher in the adventitia than in the intima.
5.
5. Anticoagulant activity, measured by thrombelastography, of the acidic glycosaminoglycans in the three layers clearly indicated that the glycosaminoglycans in the adventitia possess the highest potency among the three layers. It was considered that the higher activity of the glycosaminoglycans in the adventitia is due to the relatively greater proportion of heparan sulfates and dermatan sulfate.</description><subject>Acidic glycosaminoglycans</subject><subject>Animals</subject><subject>Anticoagulant activity</subject><subject>Aorta - analysis</subject><subject>Arterial wall</subject><subject>Blood Coagulation - drug effects</subject><subject>Cattle</subject><subject>Dermatan sulfate</subject><subject>Dermatan Sulfate - analysis</subject><subject>Electrophoresis</subject><subject>Glycosaminoglycans - analysis</subject><subject>Glycosaminoglycans - pharmacology</subject><subject>Heparan Sulfate</subject><subject>Heparin</subject><subject>Heparin - analysis</subject><subject>Heparitin Sulfate - analysis</subject><subject>Hyaluronic acid</subject><subject>Hyaluronic Acid - analysis</subject><subject>Thrombelastography</subject><issn>0021-9150</issn><issn>1879-1484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9PGzEQxS1EW9KUb5CDTxWVutTeXe-uL0gohbYSEhd6tvxnNgxK1qntjcSNj443icqN04w97z17foQsOLvkjDc_GCt5IblgF634JhmTTSFPyIx3rSx43dWnZPZfckY-x_jEGKtb3n0iH3lZ81bMyMtPjCmgGRP6gfqeaosOLV2tn62PeoODn1o9RIoDTY-QS8KN_k434FBTPTiq3Q7yZcrHHGD8Dgeg2od0GGcThtwltF6vxnXu6Db4LYSEEL-QD71eRzg_1jn5e3vzsPxd3N3_-rO8vitsJZpUuF73rIemktAxo3njTG9KLXhramhrY8reOai1M85V0ljGqsa6xjbgtHSGV3Py9ZCbn_43Qkxqg9HCOn8H_BhVV3ZcCCGzsD4IbfAxBujVNuSFw7PiTE3c1QRVTVBVK9Seu5psi2P-aDKaN9MedB5fHcaQd9whBBUtwmAzxAA2Kefx_fxX3sSWiw</recordid><startdate>1975</startdate><enddate>1975</enddate><creator>Murata, Katsumi</creator><creator>Nakazawa, Koji</creator><creator>Hamai, Akio</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1975</creationdate><title>Distribution of acidic glycosaminoglycans in the intima, media and adventitia of bovine aorta and their anticoagulant properties</title><author>Murata, Katsumi ; Nakazawa, Koji ; Hamai, Akio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-dfaf0fe639e80ba16dbfb2a517b4e74bb2fdde4adbdd39bc0036cd6c6eda9db13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Acidic glycosaminoglycans</topic><topic>Animals</topic><topic>Anticoagulant activity</topic><topic>Aorta - analysis</topic><topic>Arterial wall</topic><topic>Blood Coagulation - drug effects</topic><topic>Cattle</topic><topic>Dermatan sulfate</topic><topic>Dermatan Sulfate - analysis</topic><topic>Electrophoresis</topic><topic>Glycosaminoglycans - analysis</topic><topic>Glycosaminoglycans - pharmacology</topic><topic>Heparan Sulfate</topic><topic>Heparin</topic><topic>Heparin - analysis</topic><topic>Heparitin Sulfate - analysis</topic><topic>Hyaluronic acid</topic><topic>Hyaluronic Acid - analysis</topic><topic>Thrombelastography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murata, Katsumi</creatorcontrib><creatorcontrib>Nakazawa, Koji</creatorcontrib><creatorcontrib>Hamai, Akio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Atherosclerosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murata, Katsumi</au><au>Nakazawa, Koji</au><au>Hamai, Akio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of acidic glycosaminoglycans in the intima, media and adventitia of bovine aorta and their anticoagulant properties</atitle><jtitle>Atherosclerosis</jtitle><addtitle>Atherosclerosis</addtitle><date>1975</date><risdate>1975</risdate><volume>21</volume><issue>1</issue><spage>93</spage><epage>103</epage><pages>93-103</pages><issn>0021-9150</issn><eissn>1879-1484</eissn><abstract>1.
1. The constituents of acidic glycosaminoglycans in the tunica intima, media and adventitia were studied by means of an enzymatic assay with chondroitinases and by electrophoretic characterization.
2.
2. The content of the acidic glycosaminoglycans was higher in the intima in the adventitia based on defatted dry tissue weight. filtration pattern showed that, with chondroitinase-AC, the proportion of the intact glycosaminoglycans is greater in the adventitia than in the intima.
3.
3. Electrophoresis before and after digestion with chondroitinases indicated that the three layers contain chondroitin-4- and -6-sulfates, dermatan sulfates heparan sulfates and hyaluronic acid. In the case of the adventitia, bands migrating like the heparin standard were also detected.
4.
4. Paper chromatographic separation of the unsaturated disaccharides after digestion with chondroitinases revealed that the proportion of chondroitin-6-sulfate is predominant in the intima, whereas the proportion of heparan sulfates, hyaluronic acid and dermatan sulfate is
2
1
2
−2
times higher in the adventitia than in the intima.
5.
5. Anticoagulant activity, measured by thrombelastography, of the acidic glycosaminoglycans in the three layers clearly indicated that the glycosaminoglycans in the adventitia possess the highest potency among the three layers. It was considered that the higher activity of the glycosaminoglycans in the adventitia is due to the relatively greater proportion of heparan sulfates and dermatan sulfate.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>124175</pmid><doi>10.1016/0021-9150(75)90096-9</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Atherosclerosis, 1975, Vol.21 (1), p.93-103 |
| issn | 0021-9150 1879-1484 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82815559 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acidic glycosaminoglycans Animals Anticoagulant activity Aorta - analysis Arterial wall Blood Coagulation - drug effects Cattle Dermatan sulfate Dermatan Sulfate - analysis Electrophoresis Glycosaminoglycans - analysis Glycosaminoglycans - pharmacology Heparan Sulfate Heparin Heparin - analysis Heparitin Sulfate - analysis Hyaluronic acid Hyaluronic Acid - analysis Thrombelastography |
| title | Distribution of acidic glycosaminoglycans in the intima, media and adventitia of bovine aorta and their anticoagulant properties |
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