Improved micromethod for plasma fibrinogen unaffected by heparin therapy
An improved micromethod for plasma fibrinogen, based on the Ellis-Stransky thrombin-clot density procedure, is presented. Modifications includes: (1) addition of Polybrene eliminates heparin interference; (2) measurement of absorbance at 340 nm provides improved sensitivity at low fibrinogen levels...
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| Veröffentlicht in: | American journal of clinical pathology 1975-03, Vol.63 (3), p.426-433 |
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| container_end_page | 433 |
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| container_issue | 3 |
| container_start_page | 426 |
| container_title | American journal of clinical pathology |
| container_volume | 63 |
| creator | Saleem, A Krieg, A F Fretz, K |
| description | An improved micromethod for plasma fibrinogen, based on the Ellis-Stransky thrombin-clot density procedure, is presented. Modifications includes: (1) addition of Polybrene eliminates heparin interference; (2) measurement of absorbance at 340 nm provides improved sensitivity at low fibrinogen levels as well as with small volumes of plasma (40 mul.); (3) higher concentration of thrombin reduces the incubation time. Plasma fibrinogen by the proposed method compares well with a reference procedure based on total clottable protein (coefficient of correlation 0.99). |
| doi_str_mv | 10.1093/ajcp/63.3.426 |
| format | Article |
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Modifications includes: (1) addition of Polybrene eliminates heparin interference; (2) measurement of absorbance at 340 nm provides improved sensitivity at low fibrinogen levels as well as with small volumes of plasma (40 mul.); (3) higher concentration of thrombin reduces the incubation time. 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Modifications includes: (1) addition of Polybrene eliminates heparin interference; (2) measurement of absorbance at 340 nm provides improved sensitivity at low fibrinogen levels as well as with small volumes of plasma (40 mul.); (3) higher concentration of thrombin reduces the incubation time. Plasma fibrinogen by the proposed method compares well with a reference procedure based on total clottable protein (coefficient of correlation 0.99).</description><subject>Bilirubin - blood</subject><subject>Circadian Rhythm</subject><subject>Disseminated Intravascular Coagulation - blood</subject><subject>Disseminated Intravascular Coagulation - drug therapy</subject><subject>Fibrinogen - analysis</subject><subject>Hemolysis</subject><subject>Heparin - therapeutic use</subject><subject>Heparin Antagonists - administration & dosage</subject><subject>Humans</subject><subject>Hydrocarbons, Brominated - administration & dosage</subject><subject>Lipids - blood</subject><subject>Microchemistry</subject><subject>Nephelometry and Turbidimetry - methods</subject><subject>Photometry - methods</subject><subject>Polyamines - administration & dosage</subject><subject>Polymers - administration & dosage</subject><subject>Thrombin</subject><subject>Time Factors</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LAzEQhoMotVaPHoU9edttkkmT7FGK2kLBi55Dkk3slv0y2RX6701pQRiYwzy8zPsg9EhwQXAJS32ww5JDAQWj_ArNSckgF4LSazTHGNO8JAJu0V2MB4wJlZjN0IwQssKMz9Fm2w6h_3VV1tY29K0b932V-T5kQ6NjqzNfm1B3_bfrsqnT3js7Jtgcs70bdLpk494FPRzv0Y3XTXQPl71AX2-vn-tNvvt4365fdrmlUo4580ZosTLACTNcMiMkBVtZqpmgzhphBS5XlZCcA2gvLPMYSlxalga4gQV6Puemt38mF0fV1tG6ptGd66eoZGoIFMsE5mcw1YoxOK-GULc6HBXB6mROncwpDgpUMpf4p0vwZFpX_dNnVfAHMIlqHQ</recordid><startdate>197503</startdate><enddate>197503</enddate><creator>Saleem, A</creator><creator>Krieg, A F</creator><creator>Fretz, K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197503</creationdate><title>Improved micromethod for plasma fibrinogen unaffected by heparin therapy</title><author>Saleem, A ; Krieg, A F ; Fretz, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-4fb7a75b3614b684b7823cdc2a472ecb7c7095d786633af7c4f03909c49c436b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Bilirubin - blood</topic><topic>Circadian Rhythm</topic><topic>Disseminated Intravascular Coagulation - blood</topic><topic>Disseminated Intravascular Coagulation - drug therapy</topic><topic>Fibrinogen - analysis</topic><topic>Hemolysis</topic><topic>Heparin - therapeutic use</topic><topic>Heparin Antagonists - administration & dosage</topic><topic>Humans</topic><topic>Hydrocarbons, Brominated - administration & dosage</topic><topic>Lipids - blood</topic><topic>Microchemistry</topic><topic>Nephelometry and Turbidimetry - methods</topic><topic>Photometry - methods</topic><topic>Polyamines - administration & dosage</topic><topic>Polymers - administration & dosage</topic><topic>Thrombin</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saleem, A</creatorcontrib><creatorcontrib>Krieg, A F</creatorcontrib><creatorcontrib>Fretz, K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saleem, A</au><au>Krieg, A F</au><au>Fretz, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved micromethod for plasma fibrinogen unaffected by heparin therapy</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>1975-03</date><risdate>1975</risdate><volume>63</volume><issue>3</issue><spage>426</spage><epage>433</epage><pages>426-433</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><abstract>An improved micromethod for plasma fibrinogen, based on the Ellis-Stransky thrombin-clot density procedure, is presented. Modifications includes: (1) addition of Polybrene eliminates heparin interference; (2) measurement of absorbance at 340 nm provides improved sensitivity at low fibrinogen levels as well as with small volumes of plasma (40 mul.); (3) higher concentration of thrombin reduces the incubation time. Plasma fibrinogen by the proposed method compares well with a reference procedure based on total clottable protein (coefficient of correlation 0.99).</abstract><cop>England</cop><pmid>1115046</pmid><doi>10.1093/ajcp/63.3.426</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9173 |
| ispartof | American journal of clinical pathology, 1975-03, Vol.63 (3), p.426-433 |
| issn | 0002-9173 1943-7722 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82803208 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | Bilirubin - blood Circadian Rhythm Disseminated Intravascular Coagulation - blood Disseminated Intravascular Coagulation - drug therapy Fibrinogen - analysis Hemolysis Heparin - therapeutic use Heparin Antagonists - administration & dosage Humans Hydrocarbons, Brominated - administration & dosage Lipids - blood Microchemistry Nephelometry and Turbidimetry - methods Photometry - methods Polyamines - administration & dosage Polymers - administration & dosage Thrombin Time Factors |
| title | Improved micromethod for plasma fibrinogen unaffected by heparin therapy |
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