Kinetics and specificity of T4 polynucleotide kinase

The kinetics of T4 polynucleotide kinase has been investigated at pH 8.0 and 37 degrees. Double reciprocal plots of initial rates vs. substrate concentrations as well as product inhibition studies have indicated that the enzyme reacts according to the ordered sequential mechanism shown in eq 2 in th...

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Veröffentlicht in:	Biochemistry (Easton) 1975-03, Vol.14 (6), p.1221-1225
Hauptverfasser:	Lillehaug, Johan R, Kleppe, Kjell
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding, Competitive Coliphages - enzymology Deoxyribonucleotides - metabolism DNA - metabolism DNA Viruses - enzymology Enzyme Induction Escherichia coli - enzymology Hydrogen-Ion Concentration Kinetics Oligonucleotides - metabolism Phosphotransferases - metabolism Polynucleotide 5'-Hydroxyl-Kinase - antagonists & inhibitors Polynucleotide 5'-Hydroxyl-Kinase - metabolism Ribonucleotides - metabolism Structure-Activity Relationship
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