Isolation and identification of 1,25-dihydroxyvitamin D2
The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from...
Gespeichert in:
| Veröffentlicht in: | Biochemistry (Easton) 1975-03, Vol.14 (6), p.1250-1256 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1256 |
|---|---|
| container_issue | 6 |
| container_start_page | 1250 |
| container_title | Biochemistry (Easton) |
| container_volume | 14 |
| creator | Jones, Glenville Schnoes, Heinrich K DeLuca, Hector F |
| description | The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from an in vitro chick kidney mitochondrial system and identified as 1,25-dihydroxyvitamin D2 by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific derivative synthesis. Its antirachitic activity equals that of 1,25-dihydroxyvitamin D3 in the rat. |
| doi_str_mv | 10.1021/bi00677a025 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_82795043</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>82795043</sourcerecordid><originalsourceid>FETCH-LOGICAL-a342t-7e4a9f56b5d99d484ff2d3ca5e45d49a4cc2ba3698e61d2d1d05d0680f4c463a3</originalsourceid><addsrcrecordid>eNpNkEtLw0AURgdRan2sXAtZ6cbovCezlNZqoaDS6na4yUxwah41k0j7742kiKvLvefwwf0QuiD4lmBK7lKPsVQKMBUHaEwExTHXWhyiMe5BTLXEx-gkhHW_cqz4CI0IVolWyRgl81AX0Pq6iqCykbeuan3us-FU5xG5oSK2_mNnm3q7-_YtlL6KpvQMHeVQBHe-n6fobfawmjzFi-fH-eR-EQPjtI2V46BzIVNhtbY84XlOLctAOC4s18CzjKbApE6cJJZaYrGwWCY45xmXDNgpuhpyN0391bnQmtKHzBUFVK7ugkmo0gJz1ouXe7FLS2fNpvElNDuzf7Xn8cB9aN32D0PzaaRiSpjVy9LM9PvrCrOlmfb-9eBDFsy67pqqf7NPM7-dm3-dsx84tm84</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82795043</pqid></control><display><type>article</type><title>Isolation and identification of 1,25-dihydroxyvitamin D2</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Jones, Glenville ; Schnoes, Heinrich K ; DeLuca, Hector F</creator><creatorcontrib>Jones, Glenville ; Schnoes, Heinrich K ; DeLuca, Hector F</creatorcontrib><description>The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from an in vitro chick kidney mitochondrial system and identified as 1,25-dihydroxyvitamin D2 by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific derivative synthesis. Its antirachitic activity equals that of 1,25-dihydroxyvitamin D3 in the rat.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00677a025</identifier><identifier>PMID: 1078978</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Chickens ; Dihydroxycholecalciferols - therapeutic use ; Ergocalciferols - analogs & derivatives ; Ergocalciferols - isolation & purification ; Ergocalciferols - physiology ; Hydroxysteroids - physiology ; Kidney - metabolism ; Male ; Mitochondria - metabolism ; Rats ; Rickets - drug therapy ; Rickets - metabolism</subject><ispartof>Biochemistry (Easton), 1975-03, Vol.14 (6), p.1250-1256</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00677a025$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00677a025$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1078978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, Glenville</creatorcontrib><creatorcontrib>Schnoes, Heinrich K</creatorcontrib><creatorcontrib>DeLuca, Hector F</creatorcontrib><title>Isolation and identification of 1,25-dihydroxyvitamin D2</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from an in vitro chick kidney mitochondrial system and identified as 1,25-dihydroxyvitamin D2 by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific derivative synthesis. Its antirachitic activity equals that of 1,25-dihydroxyvitamin D3 in the rat.</description><subject>Animals</subject><subject>Chickens</subject><subject>Dihydroxycholecalciferols - therapeutic use</subject><subject>Ergocalciferols - analogs & derivatives</subject><subject>Ergocalciferols - isolation & purification</subject><subject>Ergocalciferols - physiology</subject><subject>Hydroxysteroids - physiology</subject><subject>Kidney - metabolism</subject><subject>Male</subject><subject>Mitochondria - metabolism</subject><subject>Rats</subject><subject>Rickets - drug therapy</subject><subject>Rickets - metabolism</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLw0AURgdRan2sXAtZ6cbovCezlNZqoaDS6na4yUxwah41k0j7742kiKvLvefwwf0QuiD4lmBK7lKPsVQKMBUHaEwExTHXWhyiMe5BTLXEx-gkhHW_cqz4CI0IVolWyRgl81AX0Pq6iqCykbeuan3us-FU5xG5oSK2_mNnm3q7-_YtlL6KpvQMHeVQBHe-n6fobfawmjzFi-fH-eR-EQPjtI2V46BzIVNhtbY84XlOLctAOC4s18CzjKbApE6cJJZaYrGwWCY45xmXDNgpuhpyN0391bnQmtKHzBUFVK7ugkmo0gJz1ouXe7FLS2fNpvElNDuzf7Xn8cB9aN32D0PzaaRiSpjVy9LM9PvrCrOlmfb-9eBDFsy67pqqf7NPM7-dm3-dsx84tm84</recordid><startdate>19750325</startdate><enddate>19750325</enddate><creator>Jones, Glenville</creator><creator>Schnoes, Heinrich K</creator><creator>DeLuca, Hector F</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19750325</creationdate><title>Isolation and identification of 1,25-dihydroxyvitamin D2</title><author>Jones, Glenville ; Schnoes, Heinrich K ; DeLuca, Hector F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a342t-7e4a9f56b5d99d484ff2d3ca5e45d49a4cc2ba3698e61d2d1d05d0680f4c463a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Chickens</topic><topic>Dihydroxycholecalciferols - therapeutic use</topic><topic>Ergocalciferols - analogs & derivatives</topic><topic>Ergocalciferols - isolation & purification</topic><topic>Ergocalciferols - physiology</topic><topic>Hydroxysteroids - physiology</topic><topic>Kidney - metabolism</topic><topic>Male</topic><topic>Mitochondria - metabolism</topic><topic>Rats</topic><topic>Rickets - drug therapy</topic><topic>Rickets - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, Glenville</creatorcontrib><creatorcontrib>Schnoes, Heinrich K</creatorcontrib><creatorcontrib>DeLuca, Hector F</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, Glenville</au><au>Schnoes, Heinrich K</au><au>DeLuca, Hector F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and identification of 1,25-dihydroxyvitamin D2</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1975-03-25</date><risdate>1975</risdate><volume>14</volume><issue>6</issue><spage>1250</spage><epage>1256</epage><pages>1250-1256</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from an in vitro chick kidney mitochondrial system and identified as 1,25-dihydroxyvitamin D2 by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific derivative synthesis. Its antirachitic activity equals that of 1,25-dihydroxyvitamin D3 in the rat.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>1078978</pmid><doi>10.1021/bi00677a025</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2960 |
| ispartof | Biochemistry (Easton), 1975-03, Vol.14 (6), p.1250-1256 |
| issn | 0006-2960 1520-4995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82795043 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Chickens Dihydroxycholecalciferols - therapeutic use Ergocalciferols - analogs & derivatives Ergocalciferols - isolation & purification Ergocalciferols - physiology Hydroxysteroids - physiology Kidney - metabolism Male Mitochondria - metabolism Rats Rickets - drug therapy Rickets - metabolism |
| title | Isolation and identification of 1,25-dihydroxyvitamin D2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T07%3A33%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20identification%20of%201,25-dihydroxyvitamin%20D2&rft.jtitle=Biochemistry%20(Easton)&rft.au=Jones,%20Glenville&rft.date=1975-03-25&rft.volume=14&rft.issue=6&rft.spage=1250&rft.epage=1256&rft.pages=1250-1256&rft.issn=0006-2960&rft.eissn=1520-4995&rft_id=info:doi/10.1021/bi00677a025&rft_dat=%3Cproquest_pubme%3E82795043%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82795043&rft_id=info:pmid/1078978&rfr_iscdi=true |