A proposed mechanism of thermophily in facultative thermophiles
Glyceraldehyde-3-phosphate dehydrogenase in crude extracts of Bacillus coagulans KU, a facultative thermophile, showed marked thermolability whether the cells were grown at mesophilic or thermophilic temperature. When extracts were brought to a given ionic strength by the addition of salt and then h...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1975-02, Vol.62 (3), p.627-633 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Crabb, J.W. Murdock, A.L. Amelunxen, R.E. |
| description | Glyceraldehyde-3-phosphate dehydrogenase in crude extracts of
Bacillus coagulans
KU, a facultative thermophile, showed marked thermolability whether the cells were grown at mesophilic or thermophilic temperature. When extracts were brought to a given ionic strength by the addition of salt and then heat treated, it was possible to confer heat resistance well in excess of the thermophilic growth temperature. Disc electrophoresis is indicative that portions of the profiles are quite different between extracts of cells grown at 37° and 55°. Based on the data, a mechanism of thermophily is postulated that is different from any thus far proposed for thermophilic microorganisms. |
| doi_str_mv | 10.1016/0006-291X(75)90445-3 |
| format | Article |
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Bacillus coagulans
KU, a facultative thermophile, showed marked thermolability whether the cells were grown at mesophilic or thermophilic temperature. When extracts were brought to a given ionic strength by the addition of salt and then heat treated, it was possible to confer heat resistance well in excess of the thermophilic growth temperature. Disc electrophoresis is indicative that portions of the profiles are quite different between extracts of cells grown at 37° and 55°. Based on the data, a mechanism of thermophily is postulated that is different from any thus far proposed for thermophilic microorganisms.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(75)90445-3</identifier><identifier>PMID: 1120069</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacillus - enzymology ; Drug Stability ; Electrophoresis, Disc ; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism ; Hot Temperature ; Osmolar Concentration ; Protein Denaturation</subject><ispartof>Biochemical and biophysical research communications, 1975-02, Vol.62 (3), p.627-633</ispartof><rights>1975</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-368286a554b5d53d26db58442dcc58f884a5f8f9a04ec56dd3469922155c79453</citedby><cites>FETCH-LOGICAL-c357t-368286a554b5d53d26db58442dcc58f884a5f8f9a04ec56dd3469922155c79453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X75904453$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1120069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Crabb, J.W.</creatorcontrib><creatorcontrib>Murdock, A.L.</creatorcontrib><creatorcontrib>Amelunxen, R.E.</creatorcontrib><title>A proposed mechanism of thermophily in facultative thermophiles</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Glyceraldehyde-3-phosphate dehydrogenase in crude extracts of
Bacillus coagulans
KU, a facultative thermophile, showed marked thermolability whether the cells were grown at mesophilic or thermophilic temperature. When extracts were brought to a given ionic strength by the addition of salt and then heat treated, it was possible to confer heat resistance well in excess of the thermophilic growth temperature. Disc electrophoresis is indicative that portions of the profiles are quite different between extracts of cells grown at 37° and 55°. Based on the data, a mechanism of thermophily is postulated that is different from any thus far proposed for thermophilic microorganisms.</description><subject>Bacillus - enzymology</subject><subject>Drug Stability</subject><subject>Electrophoresis, Disc</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</subject><subject>Hot Temperature</subject><subject>Osmolar Concentration</subject><subject>Protein Denaturation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQx4Mo67r6DRR6Ej1UkzTTphdlWXzBghcFbyGbTNlIXybtwn57u1bUk6c5_B8z8yPklNErRll6TSlNY56zt4sMLnMqBMTJHpkymtOYMyr2yfTHckiOQninlDGR5hMyYYwPUj4lt_Oo9U3bBLRRhWataxeqqCmibo2-atq1K7eRq6NCm77sdOc2-EfCcEwOCl0GPPmeM_J6f_eyeIyXzw9Pi_kyNglkXZykkstUA4gVWEgsT-0KpBDcGgOykFJoKGSRayrQQGptMtyZc84ATJYLSGbkfOwdrv3oMXSqcsFgWeoamz4oyTMJVPDBKEaj8U0IHgvVeldpv1WMqh03tYOidlBUBuqLm0qG2Nl3f7-q0P6GRlCDfjPqODy5cehVMA5rg9Z5NJ2yjft_wSdeUHvf</recordid><startdate>19750203</startdate><enddate>19750203</enddate><creator>Crabb, J.W.</creator><creator>Murdock, A.L.</creator><creator>Amelunxen, R.E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750203</creationdate><title>A proposed mechanism of thermophily in facultative thermophiles</title><author>Crabb, J.W. ; Murdock, A.L. ; Amelunxen, R.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-368286a554b5d53d26db58442dcc58f884a5f8f9a04ec56dd3469922155c79453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Bacillus - enzymology</topic><topic>Drug Stability</topic><topic>Electrophoresis, Disc</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</topic><topic>Hot Temperature</topic><topic>Osmolar Concentration</topic><topic>Protein Denaturation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crabb, J.W.</creatorcontrib><creatorcontrib>Murdock, A.L.</creatorcontrib><creatorcontrib>Amelunxen, R.E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crabb, J.W.</au><au>Murdock, A.L.</au><au>Amelunxen, R.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A proposed mechanism of thermophily in facultative thermophiles</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1975-02-03</date><risdate>1975</risdate><volume>62</volume><issue>3</issue><spage>627</spage><epage>633</epage><pages>627-633</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Glyceraldehyde-3-phosphate dehydrogenase in crude extracts of
Bacillus coagulans
KU, a facultative thermophile, showed marked thermolability whether the cells were grown at mesophilic or thermophilic temperature. When extracts were brought to a given ionic strength by the addition of salt and then heat treated, it was possible to confer heat resistance well in excess of the thermophilic growth temperature. Disc electrophoresis is indicative that portions of the profiles are quite different between extracts of cells grown at 37° and 55°. Based on the data, a mechanism of thermophily is postulated that is different from any thus far proposed for thermophilic microorganisms.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>1120069</pmid><doi>10.1016/0006-291X(75)90445-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1975-02, Vol.62 (3), p.627-633 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82785042 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacillus - enzymology Drug Stability Electrophoresis, Disc Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Hot Temperature Osmolar Concentration Protein Denaturation |
| title | A proposed mechanism of thermophily in facultative thermophiles |
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