The early effects of ouabain on potassium metabolism and rate of proliferation of mouse lymphoblasts

Murine lymphoblasts grown in suspension culture in the presence of ouabain showed a dose dependent and sequential decrease in 86Rb+ (K+ analogue) influx, cellular potassium content, and growth rate. An increase in eosin staining and a decrease in cell number was observed after two hours in the prese...

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Veröffentlicht in:	J. Cell. Physiol.; (United States) 1975-04, Vol.85 (2), p.209-215
Hauptverfasser:	Cuff, Joyce M., Lichtman, Marshall A.
Format:	Artikel
Sprache:	eng
Schlagworte:	550301 - Cytology- Tracer Techniques 550501 - Metabolism- Tracer Techniques ALKALI METAL ISOTOPES ALKALI METALS ANIMAL CELLS ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOLOGICAL EFFECTS BIOLOGICAL MATERIALS BLOOD BLOOD CELLS BODY FLUIDS CARBOHYDRATES CARDIOTONICS Cell Count Cell Division - drug effects CELL PROLIFERATION Cells, Cultured CONNECTIVE TISSUE CELLS DAYS LIVING RADIOISOTOPES Dose-Response Relationship, Drug DRUGS ELEMENTS GLYCOSIDES GROWTH INTERMEDIATE MASS NUCLEI ISOMERIC TRANSITION ISOTOPES ISOTOPES LEUKOCYTES LYMPHOCYTES Lymphoma - metabolism MAMMALS METABOLISM METALS MICE MINUTES LIVING RADIOISOTOPES NUCLEI ODD-ODD NUCLEI ORGANIC COMPOUNDS OUABAIN Ouabain - pharmacology POTASSIUM Potassium - metabolism RADIOISOTOPES RODENTS Rubidium RUBIDIUM 86 RUBIDIUM ISOTOPES SOMATIC CELLS Spectrophotometry, Atomic STEROIDS STROPHANTIN UPTAKE VERTEBRATES
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Zusammenfassung:	Murine lymphoblasts grown in suspension culture in the presence of ouabain showed a dose dependent and sequential decrease in 86Rb+ (K+ analogue) influx, cellular potassium content, and growth rate. An increase in eosin staining and a decrease in cell number was observed after two hours in the presence of 1 mM ouabain; 1 μM ouabain was without effect on any of the parameters measured. Ouabain inhibition was rapidly and completely reversible at concentrations that were not cytotoxic.
ISSN:	0021-9541 1097-4652
DOI:	10.1002/jcp.1040850207