Murine mammary gland RNA directed synthesis of casein in a heterologous cell-free protein synthesis system

A cell-free protein synthesis system derived from Ehrlich ascites tumor cell ribosomes ( S 30) plus rabbit reticulocyte tRNA was developed and the activity of the system was dependent on rabbit reticulocyte ribosomal salt (0.5 M KCl) wash factors. The exogenous mRNAs from BALB/c mouse liver and the...

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Veröffentlicht in:	Cell differentiation 1975-05, Vol.4 (2), p.113-122
Hauptverfasser:	Terry, P.M., Ganguly, R., Ball, E.M., Banerjee, M.R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carcinoma, Ehrlich Tumor - metabolism Caseins - biosynthesis Caseins - immunology Cell-Free System Electrophoresis, Polyacrylamide Gel Female In Vitro Techniques Iodine Radioisotopes Lactation Leucine - metabolism Liver - metabolism Mammary Glands, Animal - metabolism Mice Mice, Inbred BALB C Peptide Initiation Factors - metabolism Pregnancy Protein Biosynthesis Rabbits Radioimmunoassay Reticulocytes - metabolism Ribosomes - metabolism RNA, Transfer - metabolism Tritium
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